BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060372.seq (680 letters) Database: tribolium 336 sequences; 122,585 total letters Searching.......................................................done Score E Sequences producing significant alignments: (bits) Value AY887136-1|AAW78361.1| 580|Tribolium castaneum vasa RNA helicas... 46 2e-07 AJ005083-1|CAB65469.1| 585|Tribolium castaneum signal receptor ... 22 5.3 EF468474-1|ABR25244.1| 516|Tribolium castaneum methoprene-toler... 21 7.1 >AY887136-1|AAW78361.1| 580|Tribolium castaneum vasa RNA helicase protein. Length = 580 Score = 46.4 bits (105), Expect = 2e-07 Identities = 27/75 (36%), Positives = 40/75 (53%) Frame = +1 Query: 355 EVTVSGVEXHNXIQYFEEANFPDYVQQGVKTMGYKEPTPIQAQXLADSYVWKXLVGRTQT 534 EV V+G + I FE + ++ + VK GY +PT IQ + + L+ QT Sbjct: 145 EVKVTGNDAPPPITSFETSGLRPHLLENVKKSGYTKPTAIQKYAIPVILSGRDLMSCAQT 204 Query: 535 GSGKTLAYILAAIVH 579 GSGKT A++L I+H Sbjct: 205 GSGKTAAFML-PIIH 218 Score = 21.0 bits (42), Expect = 9.3 Identities = 8/12 (66%), Positives = 11/12 (91%) Frame = +2 Query: 632 VLAPTRELAQQI 667 +++PTRELA QI Sbjct: 240 IMSPTRELAIQI 251 >AJ005083-1|CAB65469.1| 585|Tribolium castaneum signal receptor protein protein. Length = 585 Score = 21.8 bits (44), Expect = 5.3 Identities = 9/31 (29%), Positives = 16/31 (51%) Frame = +3 Query: 231 RTCVAQIGFCFTPTFQQKLLXSTSYSSQKIT 323 R C +++ FC T Q + +T ++ K T Sbjct: 333 RHCESKVNFCATSPCQNGGVCTTIHAGHKCT 363 >EF468474-1|ABR25244.1| 516|Tribolium castaneum methoprene-tolerant protein. Length = 516 Score = 21.4 bits (43), Expect = 7.1 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = -2 Query: 439 HLVAHNQENLLLQSIE*XYXPQRHLQLPRXYFCT 338 HL+ H Q +L+ QSI P H +L R Y T Sbjct: 134 HLLGHLQTDLMGQSIFNITSPDDHDRL-RMYINT 166 Database: tribolium Posted date: Oct 23, 2007 1:18 PM Number of letters in database: 122,585 Number of sequences in database: 336 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 145,369 Number of Sequences: 336 Number of extensions: 2988 Number of successful extensions: 5 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5 length of database: 122,585 effective HSP length: 55 effective length of database: 104,105 effective search space used: 17801955 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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