Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060366.seq
(688 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 106 3e-24
SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a... 29 0.83
SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S... 29 0.83
SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 28 1.1
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 28 1.5
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr... 27 1.9
SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyce... 27 2.5
SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom... 27 3.4
SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce... 27 3.4
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ... 26 5.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 5.9
SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 26 5.9
SPBC725.16 |res1|sct1|MBF transcription factor complex subunit R... 25 7.8
>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
Length = 815
Score = 106 bits (254), Expect = 3e-24
Identities = 58/145 (40%), Positives = 79/145 (54%)
Frame = +1
Query: 241 LAQGQRRKETVCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 420
+ +G+RRK+TV IVL+D+ D R+ D+V+I PCP +KY +R+ +L
Sbjct: 78 VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137
Query: 421 PIDDSVEGLTGNLFEVYLKPYFMEAYRPILVTTPSWSAGACAPSSSKWSKQIHHXFASWL 600
P+ D+VEGLTG+LF+VYLKPYF+EAYRPI G+ K F
Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRKGDLFVVRGSMRQVEFKVVDVAPDEFGIVS 197
Query: 601 LIP*YTVNGXPIKREEEEEALNAVG 675
G PI RE+EE +L VG
Sbjct: 198 QDTIIHWEGEPINREDEESSLAEVG 222
Score = 76.2 bits (179), Expect = 4e-15
Identities = 35/61 (57%), Positives = 46/61 (75%)
Frame = +2
Query: 71 NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 250
N D S +D +TAILR+K +PN L+V++A +DDNSV+ LS ME LQLFRGDTV++K
Sbjct: 21 NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80
Query: 251 G 253
G
Sbjct: 81 G 81
Score = 48.8 bits (111), Expect = 7e-07
Identities = 22/38 (57%), Positives = 27/38 (71%)
Frame = +3
Query: 510 RDDTFMVRGGMRAVEFKVVETDPSPFCIVAPDTVIHCE 623
+ D F+VRG MR VEFKVV+ P F IV+ DT+IH E
Sbjct: 168 KGDLFVVRGSMRQVEFKVVDVAPDEFGIVSQDTIIHWE 205
>SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein
S15a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 130
Score = 28.7 bits (61), Expect = 0.83
Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
Frame = -1
Query: 439 QLNHQLAVCGLV---FHISLKDTERWI 368
QLN ++ CG++ F++ LKD E+W+
Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90
>SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein
S15a|Schizosaccharomyces pombe|chr 1|||Manual
Length = 130
Score = 28.7 bits (61), Expect = 0.83
Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
Frame = -1
Query: 439 QLNHQLAVCGLV---FHISLKDTERWI 368
QLN ++ CG++ F++ LKD E+W+
Sbjct: 64 QLNGRINKCGVISPRFNVKLKDIEKWV 90
>SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 319
Score = 28.3 bits (60), Expect = 1.1
Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = +3
Query: 96 LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 209
L + R + DP ++ + Q + T+SWH FH +W
Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297
>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 27.9 bits (59), Expect = 1.5
Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -1
Query: 409 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 299
LV + LKD WI + + E F G ++FS+QG
Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472
>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 382
Score = 27.5 bits (58), Expect = 1.9
Identities = 21/77 (27%), Positives = 35/77 (45%), Gaps = 2/77 (2%)
Frame = +3
Query: 45 FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 218
F+++N K KW++ A+ P S + + PT+S + + SA TT +RP S
Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342
Query: 219 NSSVVTQSCSRATPQGN 269
S + + GN
Sbjct: 343 RSPPINFDGTSYDAAGN 359
>SPAC19G12.07c |rsd1||RNA-binding protein Rsd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 604
Score = 27.1 bits (57), Expect = 2.5
Identities = 17/44 (38%), Positives = 23/44 (52%)
Frame = +3
Query: 132 TDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRATPQ 263
T P VS+ + + + QS F R S +N VVT S S TP+
Sbjct: 74 TPPPVSMGYRYARSSKQS--FQRED-SGYNDDVVTNSSSHRTPR 114
>SPAC1556.02c |sdh1||succinate dehydrogenase
Sdh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 641
Score = 26.6 bits (56), Expect = 3.4
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = -3
Query: 566 DHFELDGAHAPADH-EGVVTRIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 402
DH L +H PA+ + + I A++ G T +PV P+ ++G + TRF
Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410
>SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1008
Score = 26.6 bits (56), Expect = 3.4
Identities = 11/37 (29%), Positives = 22/37 (59%)
Frame = +2
Query: 155 EEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGNAAR 265
E+AV D+N+ ++L+ + + RGD ++ G+ R
Sbjct: 247 EKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLER 283
>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 409
Score = 25.8 bits (54), Expect = 5.9
Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
Frame = +2
Query: 95 PD-DLSTAILRRKDRPNRLIVEEAVSD 172
PD D + + + RK+ PNRL++ A+S+
Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 5.9
Identities = 14/53 (26%), Positives = 30/53 (56%)
Frame = +2
Query: 68 LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 226
L +A N + L+T +RK + N ++ +A ++ N+ +++ A E+ QL+
Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819
>SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit
Bgs4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1955
Score = 25.8 bits (54), Expect = 5.9
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +2
Query: 626 VNRLNVRKKRKH*MLSGYDDI 688
+N VR++R H L GYDDI
Sbjct: 437 INGKYVRRERPHDQLIGYDDI 457
>SPBC725.16 |res1|sct1|MBF transcription factor complex subunit
Res1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 637
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +1
Query: 562 WSKQIHHXFASWLLIP*YTVNGXPIKREEEEEALNA 669
++ QIH S + + YT+NG P+ + + LNA
Sbjct: 2 YNDQIHKITYSGVEVFEYTINGFPLMKRCHDNWLNA 37
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,830,829
Number of Sequences: 5004
Number of extensions: 56894
Number of successful extensions: 184
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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