Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060366.seq
(688 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03340.1 68418.m00286 cell division cycle protein 48, putativ... 125 2e-29
At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)... 125 3e-29
At3g53230.1 68416.m05865 cell division cycle protein 48, putativ... 120 9e-28
At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak... 31 0.95
At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containi... 30 1.3
At5g10520.1 68418.m01218 protein kinase family protein contains ... 27 8.8
At1g49400.1 68414.m05537 ribosomal protein S17 family protein si... 27 8.8
>At5g03340.1 68418.m00286 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain; supporting cDNA
gi|26449351|dbj|AK117125.1|
Length = 810
Score = 125 bits (302), Expect = 2e-29
Identities = 68/148 (45%), Positives = 89/148 (60%), Gaps = 3/148 (2%)
Frame = +1
Query: 241 LAQGQRRKETVCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 420
L +G++RK+TVCI L+D+ C + KIRM DV+S+ CP VKYGKRVHIL
Sbjct: 62 LIKGKKRKDTVCIALADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHIL 121
Query: 421 PIDDSVEGLTGNLFEVYLKPYFMEAYRPILVTTPSWSAGACAPSSSKWSKQIHHXFASWL 600
P+DD+VEG+TGNLF+ YLKPYF+EAYRP+ G S K I A +
Sbjct: 122 PVDDTVEGVTGNLFDAYLKPYFLEAYRPVRKGDLFLVRGG---MRSVEFKVIETDPAEYC 178
Query: 601 LIP*YT---VNGXPIKREEEEEALNAVG 675
++ T G P+KRE+EE L+ VG
Sbjct: 179 VVAPDTEIFCEGEPVKREDEER-LDEVG 205
Score = 82.6 bits (195), Expect = 2e-16
Identities = 37/58 (63%), Positives = 48/58 (82%)
Frame = +2
Query: 80 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKG 253
+D+K+ D STAIL RK PNRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KG
Sbjct: 8 SDSKTKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPTTMEKLQLFRGDTILIKG 65
Score = 65.3 bits (152), Expect = 4e-11
Identities = 26/38 (68%), Positives = 33/38 (86%)
Frame = +3
Query: 510 RDDTFMVRGGMRAVEFKVVETDPSPFCIVAPDTVIHCE 623
+ D F+VRGGMR+VEFKV+ETDP+ +C+VAPDT I CE
Sbjct: 152 KGDLFLVRGGMRSVEFKVIETDPAEYCVVAPDTEIFCE 189
>At3g09840.1 68416.m01174 cell division cycle protein 48 (CDC48A)
(CDC48) identical to SP|P54609 Cell division cycle
protein 48 homolog {Arabidopsis thaliana}
Length = 809
Score = 125 bits (301), Expect = 3e-29
Identities = 68/148 (45%), Positives = 89/148 (60%), Gaps = 3/148 (2%)
Frame = +1
Query: 241 LAQGQRRKETVCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 420
L +G++RK+TVCI L+D+ C + KIRM DV+S+ CP VKYGKRVHIL
Sbjct: 62 LIKGKKRKDTVCIALADETCEEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGKRVHIL 121
Query: 421 PIDDSVEGLTGNLFEVYLKPYFMEAYRPILVTTPSWSAGACAPSSSKWSKQIHHXFASWL 600
P+DD+VEG+TGNLF+ YLKPYF+EAYRP+ G S K I A +
Sbjct: 122 PVDDTVEGVTGNLFDAYLKPYFLEAYRPVRKGDLFLVRGG---MRSVEFKVIETDPAEYC 178
Query: 601 LIP*YT---VNGXPIKREEEEEALNAVG 675
++ T G P+KRE+EE L+ VG
Sbjct: 179 VVAPDTEIFCEGEPVKREDEER-LDDVG 205
Score = 85.4 bits (202), Expect = 3e-17
Identities = 39/58 (67%), Positives = 49/58 (84%)
Frame = +2
Query: 80 ADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKG 253
+D+KS D STAIL RK PNRL+V+EA++DDNSVV+L A ME+LQLFRGDT+L+KG
Sbjct: 8 SDSKSKKDFSTAILERKKSPNRLVVDEAINDDNSVVSLHPATMEKLQLFRGDTILIKG 65
Score = 65.3 bits (152), Expect = 4e-11
Identities = 26/38 (68%), Positives = 33/38 (86%)
Frame = +3
Query: 510 RDDTFMVRGGMRAVEFKVVETDPSPFCIVAPDTVIHCE 623
+ D F+VRGGMR+VEFKV+ETDP+ +C+VAPDT I CE
Sbjct: 152 KGDLFLVRGGMRSVEFKVIETDPAEYCVVAPDTEIFCE 189
>At3g53230.1 68416.m05865 cell division cycle protein 48, putative /
CDC48, putative very strong similarity to SP|P54609 Cell
division cycle protein 48 homolog {Arabidopsis
thaliana}; contains Pfam profiles PF00004: ATPase AAA
family, PF02359: Cell division protein 48 (CDC48)
N-terminal domain
Length = 815
Score = 120 bits (289), Expect = 9e-28
Identities = 65/148 (43%), Positives = 88/148 (59%), Gaps = 3/148 (2%)
Frame = +1
Query: 241 LAQGQRRKETVCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 420
L +G++RK+TVCI L+D+ C + KIRM DV+S+ CP VKYG RVHIL
Sbjct: 63 LIKGKKRKDTVCIALADETCDEPKIRMNKVVRSNLRVRLGDVISVHQCPDVKYGNRVHIL 122
Query: 421 PIDDSVEGLTGNLFEVYLKPYFMEAYRPILVTTPSWSAGACAPSSSKWSKQIHHXFASWL 600
P+DD++EG++GN+F+ YLKPYF+EAYRP+ G S K I A +
Sbjct: 123 PLDDTIEGVSGNIFDAYLKPYFLEAYRPVRKGDLFLVRGG---MRSIEFKVIETDPAEYC 179
Query: 601 LIP*YT---VNGXPIKREEEEEALNAVG 675
++ T G PIKRE+EE L+ VG
Sbjct: 180 VVAPDTEIFCEGEPIKREDEER-LDEVG 206
Score = 72.9 bits (171), Expect = 2e-13
Identities = 35/59 (59%), Positives = 47/59 (79%), Gaps = 1/59 (1%)
Frame = +2
Query: 80 ADNK-SPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKG 253
+D+K + D STAIL +K NRL+V+EA++DDNSVV+L ME+LQLFRGDT+L+KG
Sbjct: 8 SDSKGTKKDFSTAILEKKKAANRLVVDEAINDDNSVVSLHPDTMEKLQLFRGDTILIKG 66
Score = 64.9 bits (151), Expect = 5e-11
Identities = 25/38 (65%), Positives = 33/38 (86%)
Frame = +3
Query: 510 RDDTFMVRGGMRAVEFKVVETDPSPFCIVAPDTVIHCE 623
+ D F+VRGGMR++EFKV+ETDP+ +C+VAPDT I CE
Sbjct: 153 KGDLFLVRGGMRSIEFKVIETDPAEYCVVAPDTEIFCE 190
>At4g13885.1 68417.m02151 3'-5' exonuclease-related contains weak
similarity to Pfam domain PF01612: 3'-5' exonuclease
Length = 263
Score = 30.7 bits (66), Expect = 0.95
Identities = 13/23 (56%), Positives = 18/23 (78%)
Frame = -1
Query: 232 TTEELKLLHFGL*KCHD*VVIAD 164
TTEELK+ H+ L KC D +V+A+
Sbjct: 3 TTEELKISHYKLYKCFDFLVVAN 25
>At3g26630.1 68416.m03328 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile: PF01535 PPR repeat
Length = 455
Score = 30.3 bits (65), Expect = 1.3
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -2
Query: 387 RTRSDGYHIRKTHTKVVSHNTVHPNFLIRAII 292
RT S+ +++ HTK++ HN + L+R +I
Sbjct: 28 RTCSNFSQLKQIHTKIIKHNLTNDQLLVRQLI 59
>At5g10520.1 68418.m01218 protein kinase family protein contains
protein kinase domain, INTERPRO:IPR000719
Length = 467
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
Frame = +3
Query: 75 KWQIIKALMIYR-PRSSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNF 218
++ +I L Y R ++ R P L+ +SA T +++ +P W NF
Sbjct: 95 RFSVIPLLASYELTRKNLRRKQP--KLTPSESAFTCEAFFMAKPSWRNF 141
>At1g49400.1 68414.m05537 ribosomal protein S17 family protein
similar to 40S ribosomal protein S17 GI:1620985 from
[Nicotiana plumbaginifolia]
Length = 116
Score = 27.5 bits (58), Expect = 8.8
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = +3
Query: 54 SNNKF*IKWQIIKALMIYRPRSSVARTDPTVSLSKKQSAMTTQS 185
S NK I +IIK IY P+++ A + S S SA TT S
Sbjct: 64 SKNKHWIVAEIIKKARIYSPKAAAAAVSASASAS---SASTTDS 104
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,026,997
Number of Sequences: 28952
Number of extensions: 310632
Number of successful extensions: 789
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 763
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 789
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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