BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060362.seq (497 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 154 8e-39 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 81 1e-16 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 69 4e-13 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 54 9e-09 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 49 4e-07 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 43 2e-05 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 40 2e-04 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.22 SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.39 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 27 1.2 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 2.1 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 27 2.1 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 26 2.7 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 26 3.6 SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 25 8.4 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 154 bits (373), Expect = 8e-39 Identities = 77/130 (59%), Positives = 86/130 (66%) Frame = +1 Query: 88 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEHRAK 267 +EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDE ++K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 Query: 268 XXXXXXXXXXXXXXXLTGMTWRRFGIIPSTMSCVSPPRNTPVLLTEAPLNPKANRXKXTQ 447 + P P LLTEAPLNPK+NR K TQ Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121 Query: 448 IMFETFNTPA 477 I+FETFN PA Sbjct: 122 IIFETFNAPA 131 Score = 101 bits (242), Expect = 6e-23 Identities = 43/44 (97%), Positives = 43/44 (97%) Frame = +3 Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 386 AQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEH Sbjct: 58 AQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEH 101 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 80.6 bits (190), Expect = 1e-16 Identities = 45/126 (35%), Positives = 61/126 (48%), Gaps = 1/126 (0%) Frame = +1 Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEHRAKXXXXXX 285 + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG E + Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71 Query: 286 XXXXXXXXXLTGMTWRR-FGIIPSTMSCVSPPRNTPVLLTEAPLNPKANRXKXTQIMFET 462 + I S + P P+LLTE PL N+ K + +ET Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131 Query: 463 FNTPAM 480 N PA+ Sbjct: 132 LNVPAL 137 Score = 53.6 bits (123), Expect = 2e-08 Identities = 22/45 (48%), Positives = 32/45 (71%), Gaps = 1/45 (2%) Frame = +3 Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEH 386 AQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEH Sbjct: 62 AQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 68.9 bits (161), Expect = 4e-13 Identities = 45/126 (35%), Positives = 57/126 (45%), Gaps = 2/126 (1%) Frame = +1 Query: 94 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEHRAK 267 E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDE A Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61 Query: 268 XXXXXXXXXXXXXXXLTGMTWRRFGIIPSTMSCVSPPRNTPVLLTEAPLNPKANRXKXTQ 447 + PR +LLTE P+NP ANR K + Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121 Query: 448 IMFETF 465 MFE + Sbjct: 122 TMFERY 127 Score = 48.0 bits (109), Expect = 8e-07 Identities = 15/41 (36%), Positives = 33/41 (80%) Frame = +3 Query: 255 AQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAP 377 A++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P Sbjct: 58 AEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDP 98 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 54.4 bits (125), Expect = 9e-09 Identities = 37/129 (28%), Positives = 57/129 (44%) Frame = +1 Query: 91 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEHRAKX 270 +EV+A+V+D GS + GF+G+D P+ V PS G + G + Y+ + Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYIYKSNPGME 64 Query: 271 XXXXXXXXXXXXXXLTGMTWRRFGIIPSTMSCVSPPRNTPVLLTEAPLNPKANRXKXTQI 450 +T W R+G+ + P P+L+TE NP NR K + Sbjct: 65 IKNAIRNGWVENWDVTVDLW-RYGL---EQQLKTNPLEHPILITEPFDNPPENRVKTLET 120 Query: 451 MFETFNTPA 477 MFE+ PA Sbjct: 121 MFESLRCPA 129 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 48.8 bits (111), Expect = 4e-07 Identities = 17/36 (47%), Positives = 23/36 (63%) Frame = +3 Query: 279 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 386 +L YPI HG + NWD ME+ W + + LR PE+H Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDH 124 Score = 42.7 bits (96), Expect = 3e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +1 Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGDE 255 +++DNG+G K G+AG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 Score = 38.3 bits (85), Expect = 6e-04 Identities = 17/31 (54%), Positives = 20/31 (64%) Frame = +1 Query: 376 PRNTPVLLTEAPLNPKANRXKXTQIMFETFN 468 P + LLTE PLNP NR +IMFE+FN Sbjct: 121 PEDHYFLLTEPPLNPPENRENTAEIMFESFN 151 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 43.2 bits (97), Expect = 2e-05 Identities = 28/125 (22%), Positives = 55/125 (44%) Frame = +1 Query: 106 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEHRAKXXXXXX 285 LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++ + Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR----KLSRTSTLVGNDTLIEVGSRSI 82 Query: 286 XXXXXXXXXLTGMTWRRFGIIPSTMSCVSPPRNTPVLLTEAPLNPKANRXKXTQIMFETF 465 ++ + + + P+ +TE NP R T+++FE + Sbjct: 83 ARSPFERNVISNWDLMEQVLDYTFLKLGIDRMEHPICMTEPLANPTYVRSTMTELLFELY 142 Query: 466 NTPAM 480 N P++ Sbjct: 143 NAPSV 147 Score = 29.5 bits (63), Expect = 0.29 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = +3 Query: 291 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 386 P E +++NWD ME++ +TF +L + EH Sbjct: 86 PFERNVISNWDLMEQVLDYTFL-KLGIDRMEH 116 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 40.3 bits (90), Expect = 2e-04 Identities = 35/135 (25%), Positives = 60/135 (44%), Gaps = 6/135 (4%) Frame = +1 Query: 91 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEHRAKX 270 EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE + Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61 Query: 271 XXXXXXXXXXXXXXL------TGMTWRRFGIIPSTMSCVSPPRNTPVLLTEAPLNPKANR 432 + T TW R G+ V+P +++TE NP++ R Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWER-GLKEKLQ--VNPTEYA-MMITEPSWNPQSVR 117 Query: 433 XKXTQIMFETFNTPA 477 + + FE + PA Sbjct: 118 QQIMEAAFEQLHVPA 132 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.22 Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Frame = -1 Query: 248 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 87 PT S P+ PT+ P P N S P PL+TTN TS+S Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455 >SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 277 Score = 29.1 bits (62), Expect = 0.39 Identities = 18/66 (27%), Positives = 30/66 (45%) Frame = -1 Query: 323 IPVSDDSVFDGVFEGQDTSFALCSSPT*ESFCPIPTITP*WRGLPTIEGNTARGASSPAK 144 +P + +V +TSFAL ++ S PT + P +E + +S+PA Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122 Query: 143 PALHIP 126 P L+ P Sbjct: 123 PQLNSP 128 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 27.5 bits (58), Expect = 1.2 Identities = 13/27 (48%), Positives = 15/27 (55%) Frame = -2 Query: 250 LLHKSPSVPYRPSRPDGGAFPRSRGTR 170 L+ K+PS PY SRP A S TR Sbjct: 309 LISKAPSYPYSSSRPSASASLASSPTR 335 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.6 bits (56), Expect = 2.1 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%) Frame = -1 Query: 251 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 135 S T +SF P+PT P LPT NT + P+ PAL Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 26.6 bits (56), Expect = 2.1 Identities = 11/33 (33%), Positives = 18/33 (54%) Frame = +3 Query: 291 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHT 389 PI+ G V +W+ ++ W H Y+ L P + T Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTT 144 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 26.2 bits (55), Expect = 2.7 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = +1 Query: 151 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 255 G+ PRA F ++ P H G+++ M KD G+E Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEE 534 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.8 bits (54), Expect = 3.6 Identities = 12/23 (52%), Positives = 14/23 (60%) Frame = -2 Query: 133 TYRSHCLLPTRQLLRRTS*ISEI 65 TYR H +PT LL T +SEI Sbjct: 168 TYRDHDAIPTATLLNPTLDVSEI 190 >SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces pombe|chr 3|||Manual Length = 401 Score = 24.6 bits (51), Expect = 8.4 Identities = 10/16 (62%), Positives = 13/16 (81%) Frame = +1 Query: 106 LVVDNGSGMCKAGFAG 153 +V+DNG+ KAGFAG Sbjct: 8 IVLDNGAYHIKAGFAG 23 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,115,964 Number of Sequences: 5004 Number of extensions: 44731 Number of successful extensions: 153 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 150 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 196153982 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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