BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060360.seq (690 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_50623| Best HMM Match : NAD_Gly3P_dh_C (HMM E-Value=0) 71 1e-12 SB_49139| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 2e-06 SB_22212| Best HMM Match : AmoA (HMM E-Value=0.6) 29 2.7 SB_10754| Best HMM Match : C2 (HMM E-Value=5.1e-40) 28 8.2 >SB_50623| Best HMM Match : NAD_Gly3P_dh_C (HMM E-Value=0) Length = 358 Score = 70.5 bits (165), Expect = 1e-12 Identities = 31/48 (64%), Positives = 37/48 (77%) Frame = +2 Query: 251 EVTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQ 394 ++TEIIN H NVKYLP +P N+ A PDVVE KDAD+L+FVVPHQ Sbjct: 53 KLTEIINTKHMNVKYLPDFLIPENIHANPDVVECVKDADILVFVVPHQ 100 Score = 52.4 bits (120), Expect = 3e-07 Identities = 22/49 (44%), Positives = 36/49 (73%), Gaps = 1/49 (2%) Frame = +3 Query: 105 KQPKNKVCIVGSGNWGSAIAKIVGRNAASLSN-FEDRVTMWVYEEIIEG 248 ++ + +V I+GSGNWGS IA+IVG+N ++ F + V M+ YEE+++G Sbjct: 3 EEGRKRVAIIGSGNWGSVIARIVGQNVKEHNDVFFEGVEMYTYEELVDG 51 Score = 43.2 bits (97), Expect = 2e-04 Identities = 22/43 (51%), Positives = 31/43 (72%), Gaps = 1/43 (2%) Frame = +1 Query: 484 RAGIDLISLLLQDA*KFP-VAVLMGANIASEVAEEKFCETTIG 609 + G+ L+S ++++A P V+VLMGAN+A EVA E F E TIG Sbjct: 106 KTGLTLVSNVIKEALDLPEVSVLMGANLAKEVARELFGEATIG 148 >SB_49139| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 772 Score = 49.6 bits (113), Expect = 2e-06 Identities = 21/44 (47%), Positives = 32/44 (72%), Gaps = 1/44 (2%) Frame = +3 Query: 120 KVCIVGSGNWGSAIAKIVGRNAASLSN-FEDRVTMWVYEEIIEG 248 KV ++GSGNWG+AIA+I+G N + F ++V M+VY+ +I G Sbjct: 229 KVTVLGSGNWGTAIARIIGDNVRKKPHLFHNKVQMYVYDSLING 272 Score = 43.2 bits (97), Expect = 2e-04 Identities = 28/76 (36%), Positives = 38/76 (50%) Frame = +2 Query: 248 KEVTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLG 427 ++++EIIN HENVK LPG K+P NV F+ ++C + Sbjct: 273 RKLSEIINTEHENVKDLPGFKIPPNV-----------------------FLDSVCQKIKS 309 Query: 428 KIKPTAAALSLIKGFD 475 IKP A+SLIKG D Sbjct: 310 SIKPDVLAISLIKGLD 325 Score = 39.5 bits (88), Expect = 0.003 Identities = 21/46 (45%), Positives = 32/46 (69%), Gaps = 1/46 (2%) Frame = +1 Query: 481 RRAGIDLISLLLQDA*KFP-VAVLMGANIASEVAEEKFCETTIGCR 615 R+ G+ L+S ++++ V+V+MGAN+A EVA+ F ETTIG R Sbjct: 327 RKKGLHLVSNQIKESLGLQHVSVMMGANLADEVAKGFFSETTIGSR 372 >SB_22212| Best HMM Match : AmoA (HMM E-Value=0.6) Length = 772 Score = 29.5 bits (63), Expect = 2.7 Identities = 23/76 (30%), Positives = 36/76 (47%), Gaps = 1/76 (1%) Frame = +2 Query: 290 KYLPGHKLPSNVVAVPDVVEAAKDAD-LLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 466 + L G+ P + P+VV+ + A L+I VVP T L G P AA + + Sbjct: 200 RQLNGNDAPMAALVGPEVVDVSVGAAALMIRVVPKFSTATFTRQLNGNDAPIAALVGP-E 258 Query: 467 GFDIAEGLASILYHYY 514 D++ G AS++ Y Sbjct: 259 VVDVSVGAASLMIRPY 274 Score = 29.1 bits (62), Expect = 3.6 Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 1/72 (1%) Frame = +2 Query: 290 KYLPGHKLPSNVVAVPDVVEAAKDA-DLLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 466 + L G+ P + P+VV+ + A L+I VVP T L G P AA + + Sbjct: 523 RQLNGNDAPMAALVGPEVVDVSVGAASLMIRVVPKFSTATFTRQLNGNDAPIAALVGP-E 581 Query: 467 GFDIAEGLASIL 502 D++ G AS++ Sbjct: 582 VVDVSVGAASLM 593 Score = 28.3 bits (60), Expect = 6.2 Identities = 22/72 (30%), Positives = 35/72 (48%), Gaps = 1/72 (1%) Frame = +2 Query: 290 KYLPGHKLPSNVVAVPDVVEAAKDAD-LLIFVVPHQFVRTICSTLLGKIKPTAAALSLIK 466 + L G+ P + P+VV+ + A L+I VVP T L G P AA + + Sbjct: 481 RQLNGNDAPMAALVGPEVVDVSVGAAALMIRVVPKFSTATFTRQLNGNDAPMAALVGP-E 539 Query: 467 GFDIAEGLASIL 502 D++ G AS++ Sbjct: 540 VVDVSVGAASLM 551 >SB_10754| Best HMM Match : C2 (HMM E-Value=5.1e-40) Length = 2057 Score = 27.9 bits (59), Expect = 8.2 Identities = 9/15 (60%), Positives = 12/15 (80%) Frame = +3 Query: 486 GWHRSYITIITRCLK 530 GWH+ ++TI RCLK Sbjct: 792 GWHKRWMTITDRCLK 806 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 19,096,993 Number of Sequences: 59808 Number of extensions: 362093 Number of successful extensions: 854 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 800 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 854 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1793485733 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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