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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060360.seq
         (690 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD...    91   6e-19
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy...    62   3e-10
At2g29770.1 68415.m03617 kelch repeat-containing F-box family pr...    31   0.72 
At5g63910.1 68418.m08025 expressed protein                             31   0.95 
At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transfera...    29   2.2  
At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transfera...    27   8.9  
At5g07800.1 68418.m00894 flavin-containing monooxygenase family ...    27   8.9  

>At5g40610.1 68418.m04930 glycerol-3-phosphate dehydrogenase [NAD+]
           / GPDH strong similarity to  SP|P52425
           Glycerol-3-phosphate dehydrogenase [NAD+] (EC 1.1.1.8)
           {Cuphea lanceolata}; contains Pfam profile PF01210:
           NAD-dependent glycerol-3-phosphate dehydrogenase
          Length = 400

 Score = 91.1 bits (216), Expect = 6e-19
 Identities = 41/77 (53%), Positives = 57/77 (74%)
 Frame = +2

Query: 248 KEVTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLG 427
           +++ ++IN+T+ENVKYLPG KL  NVVA PD+  A KDA++L+FV PHQF+  IC  L G
Sbjct: 100 EKLNDVINKTNENVKYLPGIKLGRNVVADPDLENAVKDANMLVFVTPHQFMDGICKKLDG 159

Query: 428 KIKPTAAALSLIKGFDI 478
           KI     A+SL+KG ++
Sbjct: 160 KITGDVEAISLVKGMEV 176



 Score = 61.3 bits (142), Expect = 6e-10
 Identities = 24/43 (55%), Positives = 32/43 (74%)
 Frame = +3

Query: 114 KNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEII 242
           K+KV +VGSGNWGS  AK++  NA  L +F D V MWV+EE++
Sbjct: 54  KSKVTVVGSGNWGSVAAKLIASNALKLPSFHDEVRMWVFEEVL 96



 Score = 40.7 bits (91), Expect = 9e-04
 Identities = 21/45 (46%), Positives = 28/45 (62%)
 Frame = +1

Query: 481 RRAGIDLISLLLQDA*KFPVAVLMGANIASEVAEEKFCETTIGCR 615
           ++ G  +IS L+         VLMGANIA+E+A EKF E T+G R
Sbjct: 177 KKEGPCMISSLISKQLGINCCVLMGANIANEIAVEKFSEATVGYR 221


>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
           dehydrogenase family protein low similarity to SP|Q26756
           Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
           (EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
           Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
           dehydrogenase
          Length = 420

 Score = 62.5 bits (145), Expect = 3e-10
 Identities = 30/75 (40%), Positives = 39/75 (52%)
 Frame = +2

Query: 254 VTEIINETHENVKYLPGHKLPSNVVAVPDVVEAAKDADLLIFVVPHQFVRTICSTLLGKI 433
           V + INE H N KY P HKLP NV+A  D   A  DAD  +  VP QF  +    +   +
Sbjct: 124 VCQSINENHHNCKYFPEHKLPENVIATTDAKAALLDADYCLHAVPVQFSSSFLEGIADYV 183

Query: 434 KPTAAALSLIKGFDI 478
            P    +SL KG ++
Sbjct: 184 DPGLPFISLSKGLEL 198


>At2g29770.1 68415.m03617 kelch repeat-containing F-box family
           protein contains Pfam PF00646: F-box domain; contains
           Pfam PF01344 : Kelch motif; similar to SKP1 interacting
           partner 6 (GI:10716957) [Arabidopsis thaliana]
          Length = 387

 Score = 31.1 bits (67), Expect = 0.72
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = +3

Query: 540 GCINGSQYCIGGC*GKILRNDHWL 611
           G I+G  Y IGGC   + RNDHW+
Sbjct: 179 GVIDGRIYVIGGC---VKRNDHWI 199


>At5g63910.1 68418.m08025 expressed protein
          Length = 500

 Score = 30.7 bits (66), Expect = 0.95
 Identities = 15/42 (35%), Positives = 24/42 (57%)
 Frame = +3

Query: 123 VCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEG 248
           VCIVGSG  GS++A  +   + S    + ++ M+   EI+ G
Sbjct: 38  VCIVGSGIGGSSVAHFLRNYSVSTGLNQAKILMFERHEIVGG 79


>At3g50740.1 68416.m05552 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 487

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 17/54 (31%), Positives = 26/54 (48%)
 Frame = +3

Query: 87  ILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEG 248
           +LD  +KQP   V  +  G+ GS  AK +   A  L   + R  +WV    ++G
Sbjct: 258 VLDWLNKQPDESVLYISFGSGGSLSAKQLTELAWGLEMSQQRF-VWVVRPPVDG 310


>At5g66690.1 68418.m08407 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 481

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 16/57 (28%), Positives = 26/57 (45%)
 Frame = +3

Query: 78  DCNILDMADKQPKNKVCIVGSGNWGSAIAKIVGRNAASLSNFEDRVTMWVYEEIIEG 248
           D  +LD  ++QP   V  +  G+ G   AK +   A  L   + R  +WV    ++G
Sbjct: 250 DHPVLDWLNEQPNESVLYISFGSGGCLSAKQLTELAWGLEQSQQRF-VWVVRPPVDG 305


>At5g07800.1 68418.m00894 flavin-containing monooxygenase family
           protein / FMO family protein similar to
           flavin-containing monooxygenase 2 (FMO2) from Homo
           sapiens [GI:1834493]; contains Pfam profile: PF00743
           Flavin-binding monooxygenase-like
          Length = 460

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 15/52 (28%), Positives = 23/52 (44%), Gaps = 3/52 (5%)
 Frame = +3

Query: 99  ADKQPKNKVCIVGSGNWGSAIAKIV---GRNAASLSNFEDRVTMWVYEEIIE 245
           A +    KVC++G+G  G   A+ +   G     L   ED    W Y+  +E
Sbjct: 8   ASRSRSKKVCVIGAGPAGLVSARELRKEGHKVVVLEQNEDVGGQWFYQPNVE 59


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,465,769
Number of Sequences: 28952
Number of extensions: 258460
Number of successful extensions: 699
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 679
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 699
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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