BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060359.seq (677 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 117 2e-27 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 117 2e-27 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 4.4 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 26 5.8 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 117 bits (281), Expect = 2e-27 Identities = 49/55 (89%), Positives = 51/55 (92%) Frame = +2 Query: 254 AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 418 AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 58 AGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWH 112 Score = 69.7 bits (163), Expect = 4e-13 Identities = 32/58 (55%), Positives = 42/58 (72%) Frame = +3 Query: 81 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 254 ++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++ Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 Score = 39.9 bits (89), Expect = 3e-04 Identities = 25/56 (44%), Positives = 32/56 (57%), Gaps = 1/56 (1%) Frame = +3 Query: 510 IEKIPELXLVVPDKVQEINKTKQAASX*APQGMX*YPLRXQVXR-LXAGKGKMRNR 674 IE+IPE+ LVV D VQ KTK+A + ++ R L AGKGK+RNR Sbjct: 144 IEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNR 199 Score = 27.9 bits (59), Expect = 1.4 Identities = 15/50 (30%), Positives = 29/50 (58%), Gaps = 2/50 (4%) Frame = +1 Query: 478 VPALVQARGHILKRFPSXPWLY--PTKSKRSTRPNRLHLXERLKAWXDIL 621 VP+L+ ARGH ++ P P + +S + T+ + L + +KA+ D++ Sbjct: 133 VPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTK-EAVALLKEIKAYRDVI 181 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 117 bits (281), Expect = 2e-27 Identities = 49/55 (89%), Positives = 51/55 (92%) Frame = +2 Query: 254 AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 418 AGHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH Sbjct: 58 AGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWH 112 Score = 70.5 bits (165), Expect = 2e-13 Identities = 33/58 (56%), Positives = 42/58 (72%) Frame = +3 Query: 81 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 254 ++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++ Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 Score = 39.5 bits (88), Expect = 4e-04 Identities = 25/56 (44%), Positives = 32/56 (57%), Gaps = 1/56 (1%) Frame = +3 Query: 510 IEKIPELXLVVPDKVQEINKTKQAASX*APQGMX*YPLRXQVXR-LXAGKGKMRNR 674 IE+IPE+ LVV D VQ KTK+A + ++ R L AGKGK+RNR Sbjct: 144 IEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNR 199 Score = 27.5 bits (58), Expect = 1.9 Identities = 15/50 (30%), Positives = 29/50 (58%), Gaps = 2/50 (4%) Frame = +1 Query: 478 VPALVQARGHILKRFPSXPWLY--PTKSKRSTRPNRLHLXERLKAWXDIL 621 VP+L+ ARGH ++ P P + +S + T+ + L + +KA+ D++ Sbjct: 133 VPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTK-EAVALLKEIKAYRDVV 181 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.2 bits (55), Expect = 4.4 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -2 Query: 142 APCTVSLFSEYTDTKGRATDRLISLL 65 A C V ++SEYT G ++ L++L+ Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 25.8 bits (54), Expect = 5.8 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%) Frame = -3 Query: 99 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 22 K+G LP+ SF Y + +PL Y TL+AV+ Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,446,668 Number of Sequences: 5004 Number of extensions: 45739 Number of successful extensions: 123 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 112 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 119 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 311890690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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