Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060359.seq
(677 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 118 3e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 118 3e-27
At1g76010.1 68414.m08825 expressed protein 30 1.2
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 2.1
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 29 2.1
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 2.8
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 2.8
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 29 2.8
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 29 2.8
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 29 3.7
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 29 3.7
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 28 4.9
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 4.9
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 6.5
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 6.5
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 8.6
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 118 bits (285), Expect = 3e-27
Identities = 50/55 (90%), Positives = 52/55 (94%)
Frame = +2
Query: 254 AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 418
AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 64 AGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 118
Score = 58.4 bits (135), Expect = 4e-09
Identities = 30/59 (50%), Positives = 37/59 (62%), Gaps = 2/59 (3%)
Frame = +3
Query: 84 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 254
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKK 63
Score = 32.3 bits (70), Expect = 0.30
Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +3
Query: 510 IEKIPELXLVVPDKVQEINKTKQAASX*APQGMX*YPLRXQ-VXRLXAGKGKMRNR 674
IE +PE+ LVV D + + KT A G + + + GKGKMRNR
Sbjct: 150 IENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNR 205
Score = 27.5 bits (58), Expect = 8.6
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +1
Query: 478 VPALVQARGHILKRFPSXP 534
VPALV ARGH ++ P P
Sbjct: 139 VPALVMARGHKIENVPEMP 157
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 118 bits (285), Expect = 3e-27
Identities = 50/55 (90%), Positives = 52/55 (94%)
Frame = +2
Query: 254 AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWH 418
AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 63 AGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWH 117
Score = 60.5 bits (140), Expect = 1e-09
Identities = 30/62 (48%), Positives = 41/62 (66%), Gaps = 2/62 (3%)
Frame = +3
Query: 75 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 248
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 249 KE 254
K+
Sbjct: 61 KK 62
Score = 32.3 bits (70), Expect = 0.30
Identities = 20/56 (35%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = +3
Query: 510 IEKIPELXLVVPDKVQEINKTKQAASX*APQGMX*YPLRXQ-VXRLXAGKGKMRNR 674
IE +PE+ LVV D + + KT A G + + + GKGKMRNR
Sbjct: 149 IENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNR 204
Score = 27.5 bits (58), Expect = 8.6
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +1
Query: 478 VPALVQARGHILKRFPSXP 534
VPALV ARGH ++ P P
Sbjct: 138 VPALVMARGHKIENVPEMP 156
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 30.3 bits (65), Expect = 1.2
Identities = 18/43 (41%), Positives = 19/43 (44%)
Frame = +2
Query: 248 QGAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 376
QG G + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 2.1
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +3
Query: 99 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 245
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 29.5 bits (63), Expect = 2.1
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = -2
Query: 577 CLVLLISWTLSGTTKXSSGIFSICV 503
CLVLL+S + TK SGIF+ C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 389 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 270
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 2.8
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 389 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 270
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +2
Query: 248 QGAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 382
+G+G + + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 29.1 bits (62), Expect = 2.8
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +2
Query: 248 QGAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 382
+G+G + + G GR R +GGG H+ G+G +GGR
Sbjct: 15 EGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 28.7 bits (61), Expect = 3.7
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 373 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCC 471
WW +V LA+ APS P + L SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 28.7 bits (61), Expect = 3.7
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +1
Query: 373 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCC 471
WW +V LA+ APS P + L SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 28.3 bits (60), Expect = 4.9
Identities = 18/64 (28%), Positives = 29/64 (45%), Gaps = 3/64 (4%)
Frame = +2
Query: 224 LEAALLREQGAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 394
+ AL+R + G + +AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRR-MGAKPTAEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 395 TKPW 406
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 4.9
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 150 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 245
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 8.6
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +3
Query: 150 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 245
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 6.5
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -1
Query: 377 HHDTCYRRHPDRTYEYHHHGHAEF 306
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 6.5
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = -3
Query: 153 GAWLHPAPSHSSLNTPTLKVGLPID--SFRYFSEAIPLKY 40
G W+H SH+ ++ L VG+ + Y+S + P++Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 8.6
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -1
Query: 377 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 294
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,499,027
Number of Sequences: 28952
Number of extensions: 274261
Number of successful extensions: 887
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 821
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 874
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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