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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060358.seq
         (603 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g55940.1 68418.m06977 expressed protein contains Pfam PF03665...    45   3e-05
At3g03230.1 68416.m00319 esterase/lipase/thioesterase family pro...    31   0.78 
At3g03240.1 68416.m00320 esterase/lipase/thioesterase family pro...    30   1.4  
At1g74280.1 68414.m08602 hydrolase, alpha/beta fold family prote...    27   7.2  

>At5g55940.1 68418.m06977 expressed protein contains Pfam PF03665:
           Uncharacterised protein family (UPF0172)
          Length = 208

 Score = 45.2 bits (102), Expect = 3e-05
 Identities = 27/71 (38%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
 Frame = +2

Query: 299 LDIVDAIPLFHHSHYVSPMAEVALTQIETIAQSENRVIAGYYAACENFRDNIVERCPGLK 478
           ++I D++PLFH +  + P  E++L  IE    ++   I GY+ A E F D  VE C   K
Sbjct: 49  VEISDSVPLFHSNLALLPPLEISLIMIEEHYVAQGLSIVGYFHANERFDD--VELCGVAK 106

Query: 479 -IAEKIVEYFP 508
            I + I  YFP
Sbjct: 107 NIGDHISRYFP 117



 Score = 30.3 bits (65), Expect = 1.0
 Identities = 12/26 (46%), Positives = 18/26 (69%)
 Frame = +3

Query: 180 IETGAYAKIILHAAKYPHCAVNGVLL 257
           I   AY K++LH+ ++   AVNGVL+
Sbjct: 13  ISQNAYIKLVLHSLRHKTAAVNGVLV 38


>At3g03230.1 68416.m00319 esterase/lipase/thioesterase family
           protein contains Interpro entry IPR000379
          Length = 333

 Score = 30.7 bits (66), Expect = 0.78
 Identities = 12/39 (30%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
 Frame = +2

Query: 434 ENFRDNIVERCP-GLKIAEKIVEYFPLLCXLWWVTKKWF 547
           +N  +  +++ P G ++  ++  Y P L   WW+T+KWF
Sbjct: 171 QNLLNAAMKKLPIGFQLTLRVAHYSPWLLY-WWMTQKWF 208


>At3g03240.1 68416.m00320 esterase/lipase/thioesterase family
           protein contains Interpro entry IPR000379
          Length = 333

 Score = 29.9 bits (64), Expect = 1.4
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = +2

Query: 491 IVEYFPLLCXLWWVTKKWFN 550
           +  YFP L   WW+T+KWF+
Sbjct: 191 VAHYFPWLLY-WWMTQKWFS 209


>At1g74280.1 68414.m08602 hydrolase, alpha/beta fold family protein
           contains Pfam profile PF00561: hydrolase, alpha/beta
           fold family
          Length = 372

 Score = 27.5 bits (58), Expect = 7.2
 Identities = 10/24 (41%), Positives = 15/24 (62%)
 Frame = +2

Query: 476 KIAEKIVEYFPLLCXLWWVTKKWF 547
           ++A ++  Y P L   WW T+KWF
Sbjct: 213 QLAVRVAHYTPWLIY-WWNTQKWF 235


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,946,414
Number of Sequences: 28952
Number of extensions: 199194
Number of successful extensions: 441
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 433
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 441
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1197101088
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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