BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060351.seq (621 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||... 29 0.72 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.3 SPAC3C7.06c |pit1||serine/threonine protein kinase Pit1|Schizosa... 27 2.2 SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2... 27 2.9 SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyce... 26 5.1 >SPAC10F6.03c |||CTP synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 600 Score = 28.7 bits (61), Expect = 0.72 Identities = 17/48 (35%), Positives = 24/48 (50%) Frame = +3 Query: 285 SVGGGAWPFLVGGAICLVNSGNERDSSLLNRRRYLGVRGLVSRNSLTT 428 ++ G L G + ++N G E D L N RYL V L N++TT Sbjct: 44 NIDAGTMSPLEHGEVFVLNDGGEVDLDLGNYERYLNVT-LTHDNNITT 90 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 27.9 bits (59), Expect = 1.3 Identities = 22/75 (29%), Positives = 31/75 (41%) Frame = -3 Query: 319 PTKNGHAPPPTESRKSC*SVNPPVSGPGEISRVESN*AAGSTPGGALPSIPLSFSFATIL 140 P+ + APPP + +PP S P I+ V N A ST LP S A + Sbjct: 246 PSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALA 305 Query: 139 PPESKIFGFPEAARR 95 + + P +RR Sbjct: 306 ANKKRPPPPPPPSRR 320 Score = 26.6 bits (56), Expect = 2.9 Identities = 18/66 (27%), Positives = 29/66 (43%), Gaps = 1/66 (1%) Frame = -3 Query: 328 IAPPTKNGHAPPPTESRKSC*SVNPPVSGPGEISRVESN*AAGSTPGGALPSI-PLSFSF 152 + P ++ PPP S S PP+S +S + + PG + P++ PL + Sbjct: 353 LPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPA--PPPAIPGRSAPALPPLGNAS 410 Query: 151 ATILPP 134 T PP Sbjct: 411 RTSTPP 416 Score = 25.4 bits (53), Expect = 6.7 Identities = 21/68 (30%), Positives = 29/68 (42%) Frame = -3 Query: 325 APPTKNGHAPPPTESRKSC*SVNPPVSGPGEISRVESN*AAGSTPGGALPSIPLSFSFAT 146 A P ++ A PP + + PPV P + A S P A PS+P+ A Sbjct: 394 AIPGRSAPALPPLGNASR--TSTPPVPTPPSLPPS----APPSLPPSAPPSLPMGAPAAP 447 Query: 145 ILPPESKI 122 LPP + I Sbjct: 448 PLPPSAPI 455 >SPAC3C7.06c |pit1||serine/threonine protein kinase Pit1|Schizosaccharomyces pombe|chr 1|||Manual Length = 650 Score = 27.1 bits (57), Expect = 2.2 Identities = 17/45 (37%), Positives = 25/45 (55%) Frame = -1 Query: 231 FPVLSQIKPQAPLLVVPFRQFL*VSALQPYSPRSPKSLVSRKLPA 97 FPVL QI+P P L + R F+ S+ SP++ + R LP+ Sbjct: 441 FPVLPQIRPSTP-LNLKLRNFIISSSEDSTSPKAKE--FDRPLPS 482 >SPBP19A11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 26.6 bits (56), Expect = 2.9 Identities = 15/59 (25%), Positives = 28/59 (47%) Frame = -3 Query: 298 PPPTESRKSC*SVNPPVSGPGEISRVESN*AAGSTPGGALPSIPLSFSFATILPPESKI 122 PPP+ S S + PP + + V + +T A P+ ++ S T++PP + + Sbjct: 71 PPPSTSHNSTTTTVPPTTSMNTTTTVPPTTSLNTTTTTAPPTTHVN-STTTVVPPTTHV 128 >SPAC22H10.07 |scd2|ral3|scaffold protein Scd2|Schizosaccharomyces pombe|chr 1|||Manual Length = 536 Score = 25.8 bits (54), Expect = 5.1 Identities = 14/33 (42%), Positives = 17/33 (51%) Frame = -3 Query: 199 STPGGALPSIPLSFSFATILPPESKIFGFPEAA 101 ST ALP PLSFS P ++ PE+A Sbjct: 420 STMPEALPREPLSFSLPEKAPEKATNISIPESA 452 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,473,425 Number of Sequences: 5004 Number of extensions: 49357 Number of successful extensions: 135 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 129 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 135 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 273658928 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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