Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060349.seq
         (695 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac...    99   5e-22
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr...    30   0.28 
SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a...    29   0.84 
SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S...    29   0.84 
SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi...    28   1.1  
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac...    28   1.5  
SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   4.5  
SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein ...    26   5.9  
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ...    26   5.9  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   5.9  
SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom...    25   7.8  
SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pomb...    25   7.8  
SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyce...    25   7.8  
SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex ...    25   7.8  

>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
           Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score = 99.1 bits (236), Expect = 5e-22
 Identities = 43/91 (47%), Positives = 62/91 (68%)
 Frame = +1

Query: 235 LAQGQTPKETVCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKRVHIL 414
           + +G+  K+TV IVL+D+   D   R+             D+V+I PCP +KY +R+ +L
Sbjct: 78  VVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAERISVL 137

Query: 415 PIDDSVEGLTGNLFEVYLKPYFMEAYRPIHR 507
           P+ D+VEGLTG+LF+VYLKPYF+EAYRPI +
Sbjct: 138 PLADTVEGLTGSLFDVYLKPYFVEAYRPIRK 168



 Score = 80.2 bits (189), Expect = 3e-16
 Identities = 37/63 (58%), Positives = 48/63 (76%)
 Frame = +2

Query: 65  NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFRGDTVLLK 244
           N   D  S +D +TAILR+K +PN L+V++A +DDNSV+ LS   ME LQLFRGDTV++K
Sbjct: 21  NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLFRGDTVVVK 80

Query: 245 GKR 253
           GKR
Sbjct: 81  GKR 83



 Score = 64.5 bits (150), Expect = 1e-11
 Identities = 29/49 (59%), Positives = 36/49 (73%)
 Frame = +3

Query: 510 DTFMVRGGMRAVEFKVVETDPSPFCIVAPDTVIHCDGEPIKREEEXEAL 656
           D F+VRG MR VEFKVV+  P  F IV+ DT+IH +GEPI RE+E  +L
Sbjct: 170 DLFVVRGSMRQVEFKVVDVAPDEFGIVSQDTIIHWEGEPINREDEESSL 218


>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 382

 Score = 30.3 bits (65), Expect = 0.28
 Identities = 22/77 (28%), Positives = 37/77 (48%), Gaps = 2/77 (2%)
 Frame = +3

Query: 39  FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 212
           F+++N K   KW++  A+    P  S + + PT+S   + + SA TT     +RP  S  
Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342

Query: 213 NSSVVTQSCSRANAQGN 263
            S  +    +  +A GN
Sbjct: 343 RSPPINFDGTSYDAAGN 359


>SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein
           S15a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 130

 Score = 28.7 bits (61), Expect = 0.84
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -2

Query: 433 QLNHQLAVCGLV---FHISLKDTERWI 362
           QLN ++  CG++   F++ LKD E+W+
Sbjct: 64  QLNGRINKCGVISPRFNVKLKDIEKWV 90


>SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein
           S15a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 130

 Score = 28.7 bits (61), Expect = 0.84
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -2

Query: 433 QLNHQLAVCGLV---FHISLKDTERWI 362
           QLN ++  CG++   F++ LKD E+W+
Sbjct: 64  QLNGRINKCGVISPRFNVKLKDIEKWV 90


>SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 319

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
 Frame = +3

Query: 90  LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 203
           L + R    +   DP ++ +  Q  + T+SWH FH  +W
Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297


>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
           E3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -2

Query: 403 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 293
           LV  + LKD   WI    + + E  F   G ++FS+QG
Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472


>SPBC685.03 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 452

 Score = 26.2 bits (55), Expect = 4.5
 Identities = 14/48 (29%), Positives = 26/48 (54%)
 Frame = +3

Query: 111 SSVARTDPTVSLSKKQSAMTTQSWHFHRPKWSNFNSSVVTQSCSRANA 254
           SSV       S S   ++M++ + +F+    S+ +SSV T + S +N+
Sbjct: 329 SSVVIVQSETSCSINSASMSSNTTYFYWNSTSSLSSSVFTNTTSSSNS 376


>SPAPJ698.02c |rps002|rpsa-2, rps0-2, rps0|40S ribosomal protein
           S0B|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 287

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 13/28 (46%), Positives = 16/28 (57%)
 Frame = +3

Query: 588 VAPDTVIHCDGEPIKREEEXEALNAGRA 671
           V PD   + D E I+REEE +A  A  A
Sbjct: 200 VMPDLYFYRDPEEIEREEEQKAAAAAAA 227


>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 409

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
 Frame = +2

Query: 89  PD-DLSTAILRRKDRPNRLIVEEAVSD 166
           PD D + + + RK+ PNRL++  A+S+
Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 14/53 (26%), Positives = 30/53 (56%)
 Frame = +2

Query: 62  LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 220
           L  +A N   + L+T   +RK + N  ++ +A ++ N+  +++ A  E+ QL+
Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLY 819


>SPAC1556.02c |sdh1||succinate dehydrogenase
           Sdh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 641

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
 Frame = -1

Query: 560 DHFELDGAHAPADH-EGVARWIGR*ASMKYGFKYTSNRLPVRPSTESSIGSMWTRF 396
           DH  L  +H PA+  +     I   A++  G   T   +PV P+   ++G + TRF
Sbjct: 355 DHCYLQLSHLPAEILKERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTRF 410


>SPAC17A5.14 |exo2||exonuclease II Exo2 |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1328

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 10/28 (35%), Positives = 17/28 (60%)
 Frame = +1

Query: 493  RPIHRATPSWSAGACAPSSSKWSKQIHH 576
            R + + T ++ +    PSSS W+K+ HH
Sbjct: 1231 RLVTKPTSTFPSPPSPPSSSVWNKREHH 1258


>SPCC23B6.04c |||sec14 cytosolic factor family|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1008

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 11/37 (29%), Positives = 21/37 (56%)
 Frame = +2

Query: 149 EEAVSDDNSVVALSQAKMEQLQLFRGDTVLLKGKRPR 259
           E+AV D+N+ ++L+  +  +    RGD  ++ G   R
Sbjct: 247 EKAVCDENTKISLTNTEHYKFHSLRGDVEVVVGDLER 283


>SPAC17C9.01c |nuc2|apc3, SPAC1851.01|anaphase-promoting complex
           subunit Apc3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 665

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = +2

Query: 458 KYT*SRTSWRLTVRSIVRHLHGPRGHARRRVQSGRN 565
           +Y  S+TS+R  +R  VRH +   G     +++GRN
Sbjct: 480 EYEKSKTSFRKAIRVNVRHYNAWYGLGMVYLKTGRN 515


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,847,124
Number of Sequences: 5004
Number of extensions: 57321
Number of successful extensions: 182
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 182
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 321151040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)