BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060346.seq
(660 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15) 99 4e-21
SB_14043| Best HMM Match : Extensin_1 (HMM E-Value=0.75) 31 1.1
SB_53582| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 1.1
SB_49513| Best HMM Match : Thioredoxin (HMM E-Value=1.8e-19) 30 1.5
SB_45157| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.5
SB_50787| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.4
SB_6893| Best HMM Match : PPV_E2_C (HMM E-Value=0.94) 29 3.4
SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35) 24 4.0
SB_7383| Best HMM Match : SapA (HMM E-Value=1.2e-13) 28 5.9
SB_4237| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.9
SB_26835| Best HMM Match : TGF_beta (HMM E-Value=7.3e-33) 28 5.9
SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 5.9
SB_37008| Best HMM Match : MAM (HMM E-Value=1.3999e-42) 28 7.7
SB_6613| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.7
>SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15)
Length = 710
Score = 98.7 bits (235), Expect = 4e-21
Identities = 43/60 (71%), Positives = 51/60 (85%)
Frame = +3
Query: 51 TTMGDIEDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKV 230
T ++ DT F +G+SGAS T+P QCS+LRKNG V++KGRPCKIVEMSTSKTGKHGHAKV
Sbjct: 585 TMAEELADTEFHSGESGASDTYPAQCSSLRKNGHVVIKGRPCKIVEMSTSKTGKHGHAKV 644
Score = 68.5 bits (160), Expect = 4e-12
Identities = 28/62 (45%), Positives = 43/62 (69%)
Frame = +2
Query: 329 QLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEECVIA 508
++T+I +DGYL LM DNGD R D+K+ D D+ ++R F++ + + TVLK+ GEE V+
Sbjct: 643 KVTNIEEDGYLELMDDNGDTRADIKLQDNDIAKEIRAKFEASENFMVTVLKAMGEETVVG 702
Query: 509 XK 514
K
Sbjct: 703 VK 704
>SB_14043| Best HMM Match : Extensin_1 (HMM E-Value=0.75)
Length = 568
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/46 (30%), Positives = 24/46 (52%), Gaps = 1/46 (2%)
Frame = +1
Query: 64 TSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVH-ARLLKCPH 198
T+K HT++P T P P N+ P + + +L ++H + PH
Sbjct: 168 TTKPHTTKPHTTKPHTTKPHNIDPTLPSPTLLNALLHFLYFYQAPH 213
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +1
Query: 4 TSVGHISFLTVVRFKTQQWVTSKTHTSRPETPGPQPPSPCNVRPC 138
T+ H L + KT + T+K +T++P T P+ P +PC
Sbjct: 88 TTKPHTIKLYTTKPKTTKPHTNKPYTTKPRTTKPRTTKPHTTKPC 132
>SB_53582| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 393
Score = 30.7 bits (66), Expect = 1.1
Identities = 19/52 (36%), Positives = 25/52 (48%)
Frame = +1
Query: 52 QQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHARLLKCPHPKP 207
+Q V S P P P PP+PC + PC +T +VVH+ L P P
Sbjct: 126 EQHVVSHVMHPAPPPPPPPPPAPC-MPPCHQT-----QVVHSVQLHASPPGP 171
>SB_49513| Best HMM Match : Thioredoxin (HMM E-Value=1.8e-19)
Length = 975
Score = 30.3 bits (65), Expect = 1.5
Identities = 19/54 (35%), Positives = 24/54 (44%), Gaps = 2/54 (3%)
Frame = -2
Query: 449 CRS-QYATVCRGHHPEF*GPHAGHRCQPSGSGSHHPRYQSV-GSLRASRGVRPC 294
CRS Q TV HH HRC+ SG+H R+ ++ L RPC
Sbjct: 241 CRSLQVQTVINAHHTVRNVCDICHRCRKGCSGNHDNRFGAIQARLLGGLAPRPC 294
>SB_45157| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 2870
Score = 30.3 bits (65), Expect = 1.5
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = -1
Query: 519 VCFXAXTHSSPQDFSTVHNNSLPLSKSVRNCVPRSPS 409
+CF + S P + H+N+ P S NC P PS
Sbjct: 1290 ICFTSCKPSCPVHCCSEHSNACPQECSTDNCKPSCPS 1326
>SB_50787| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 599
Score = 29.1 bits (62), Expect = 3.4
Identities = 16/58 (27%), Positives = 26/58 (44%)
Frame = -2
Query: 308 GVRPCCVWRDRYLHTFYHEDINPNQVNFSVAVLSGFGCGHFNNLAWTTLQHNETVFTQ 135
G+R C D L F + + + + S A ++ +G GH N + L H + TQ
Sbjct: 243 GLRSCITHGDDLLSVFDNMESSSKVFDMSNAPVTRYGLGHVNQILEEELSHLRFLKTQ 300
>SB_6893| Best HMM Match : PPV_E2_C (HMM E-Value=0.94)
Length = 1058
Score = 29.1 bits (62), Expect = 3.4
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +1
Query: 31 TVVRFKTQQWVTSKTHTSRPETPGPQPPSPCNVRP 135
+VV + VT K T +P TP P P P RP
Sbjct: 758 SVVAMPAARPVTPKPVTPKPVTPKPVTPKPVTTRP 792
>SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35)
Length = 458
Score = 23.8 bits (49), Expect(2) = 4.0
Identities = 8/13 (61%), Positives = 9/13 (69%)
Frame = +1
Query: 97 PGPQPPSPCNVRP 135
PGPQ P P N+ P
Sbjct: 362 PGPQDPGPGNILP 374
Score = 23.4 bits (48), Expect(2) = 4.0
Identities = 11/43 (25%), Positives = 21/43 (48%)
Frame = +1
Query: 115 SPCNVRPCVKTVSLC*RVVHARLLKCPHPKPESTATLKFTWLG 243
+PC ++ C + +S+ V ++K P P TL ++G
Sbjct: 398 NPCRIQYCTQEISMTPIHVLLLIVKAPILDPSLVVTLCSRFIG 440
>SB_7383| Best HMM Match : SapA (HMM E-Value=1.2e-13)
Length = 492
Score = 28.3 bits (60), Expect = 5.9
Identities = 17/44 (38%), Positives = 22/44 (50%)
Frame = -1
Query: 489 PQDFSTVHNNSLPLSKSVRNCVPRSPSGILRSSRRSPLSAIRVR 358
P+ S V + +LP+ V C+P SPS I RS P VR
Sbjct: 253 PRQISNVRSLTLPVRYQVIGCLP-SPSDIKRSVPYPPRQISNVR 295
>SB_4237| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1438
Score = 28.3 bits (60), Expect = 5.9
Identities = 19/82 (23%), Positives = 33/82 (40%)
Frame = +2
Query: 281 PSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGKE 460
P + + V + +++ D + ADN + RE+ + +L ++ T G
Sbjct: 284 PESDGIRVDETENGEHEAVDELPEDKPDTEADNYEQREETPTKEDELKSECTTSDSEGTP 343
Query: 461 LLCTVLKSCGEECVIAXKQTQL 526
T KS GEE V + L
Sbjct: 344 SAATYGKSDGEENVAQESEESL 365
>SB_26835| Best HMM Match : TGF_beta (HMM E-Value=7.3e-33)
Length = 657
Score = 28.3 bits (60), Expect = 5.9
Identities = 19/56 (33%), Positives = 24/56 (42%), Gaps = 3/56 (5%)
Frame = -2
Query: 353 HHPRY-QSVGSLRASRGVRPCCVWRDRY--LHTFYHEDINPNQVNFSVAVLSGFGC 195
HH Q V LR R + PCC + +Y +D N Q N S V+ GC
Sbjct: 601 HHTEVIQKVALLRKQRELSPCCAPTKMFDLSLLYYDKDENLFQENVSNMVVEECGC 656
>SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 154
Score = 28.3 bits (60), Expect = 5.9
Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +3
Query: 141 KNGFVMLKGRPCKIV-EMSTSKTGKHGHAKVHLVG 242
+ G +M +G+PCKI + K G HG +H+ G
Sbjct: 17 RRGVMMAEGKPCKITGTIEGLKAGNHGF-HIHVYG 50
>SB_37008| Best HMM Match : MAM (HMM E-Value=1.3999e-42)
Length = 382
Score = 27.9 bits (59), Expect = 7.7
Identities = 9/21 (42%), Positives = 16/21 (76%)
Frame = +1
Query: 67 SKTHTSRPETPGPQPPSPCNV 129
++T T++PET P+PP+P +
Sbjct: 165 TETTTTKPETKPPKPPAPSTI 185
>SB_6613| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 523
Score = 27.9 bits (59), Expect = 7.7
Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 4/72 (5%)
Frame = +2
Query: 296 MDVPHVKREDYQLTDISDDGYLT----LMADNGDLREDLKIPDGDLGTQLRTDFDSGKEL 463
+D+P+ K + + L ++DD L+ + D L E L PD + R D G E
Sbjct: 37 LDIPYEKYDKFDLESLTDDECLSEFRFIKNDLYRLNEALNFPD-QITCPNRLTVD-GMEA 94
Query: 464 LCTVLKSCGEEC 499
LC L+ C
Sbjct: 95 LCMTLRRFAYPC 106
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,279,999
Number of Sequences: 59808
Number of extensions: 451916
Number of successful extensions: 1397
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1231
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1387
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1693527500
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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