Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060346.seq
(660 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 104 5e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 102 2e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 101 3e-22
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.7
At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 3.6
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.6
At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,... 29 3.6
At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 6.3
At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 28 6.3
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 6.3
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 8.4
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 8.4
At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 8.4
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 104 bits (250), Expect = 5e-23
Identities = 47/86 (54%), Positives = 65/86 (75%), Gaps = 1/86 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLR 436
KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q++
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIK 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIAXK 514
+ FD GK+L+ +V+ + GEE + A K
Sbjct: 128 SGFDDGKDLVVSVMSAMGEEQINALK 153
Score = 104 bits (249), Expect = 6e-23
Identities = 43/72 (59%), Positives = 56/72 (77%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 102 bits (244), Expect = 2e-22
Identities = 46/87 (52%), Positives = 65/87 (74%), Gaps = 1/87 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 436
KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L TQL+
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLK 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIAXKQ 517
F+ GK+++ +V+ + GEE + A K+
Sbjct: 128 NGFEEGKDIVVSVMSAMGEEQMCALKE 154
Score = 100 bits (240), Expect = 7e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 101 bits (243), Expect = 3e-22
Identities = 45/87 (51%), Positives = 64/87 (73%), Gaps = 1/87 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 436
KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMR 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIAXKQ 517
FD GK+++ +V+ S GEE + A K+
Sbjct: 128 LGFDEGKDIVVSVMSSMGEEQICAVKE 154
Score = 96.3 bits (229), Expect = 2e-20
Identities = 40/72 (55%), Positives = 52/72 (72%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +2
Query: 398 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 499
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g41460.1 68418.m05035 fringe-related protein strong similarity
to unknown protein (pir||T13026) similarity to predicted
proteins + similar to hypothetical protein GB:AAC23643
[Arabidopsis thaliana] + weak similarity to Fringe
[Schistocerca gregaria](GI:6573138);Fringe encodes an
extracellular protein that regulates Notch signalling.
Length = 524
Score = 28.7 bits (61), Expect = 3.6
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +1
Query: 34 VVRFKTQQWVTSKTHTSRPETPGPQPPSP 120
++ F T Q K++ S P +P P PP P
Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -3
Query: 169 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 62
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,
putative strong similarity to SP|P54151 Peptide
methionine sulfoxide reductase (EC 1.8.4.6) {Brassica
napus}; contains Pfam profile PF01625: Peptide
methionine sulfoxide reductase
Length = 258
Score = 28.7 bits (61), Expect = 3.6
Identities = 21/65 (32%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
Frame = +2
Query: 251 LHGKKYEDICPST--HNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLG 424
+H YED+C T HN V R Y + S + L + + D L GD+G
Sbjct: 126 VHNPSYEDVCTGTTGHN----EVVRVQYDPKECSFESLLDVFWNRHD-PTTLNRQGGDVG 180
Query: 425 TQLRT 439
TQ R+
Sbjct: 181 TQYRS 185
>At3g53330.1 68416.m05884 plastocyanin-like domain-containing
protein similar to mavicyanin SP:P80728 from [Cucurbita
pepo]
Length = 310
Score = 27.9 bits (59), Expect = 6.3
Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%)
Frame = +1
Query: 67 SKTHT-SRPETPGPQPPS 117
SKTH SRP TP P PPS
Sbjct: 127 SKTHERSRPITPSPPPPS 144
>At2g27680.1 68415.m03354 aldo/keto reductase family protein
contains Pfam profile PF00248: oxidoreductase, aldo/keto
reductase family
Length = 384
Score = 27.9 bits (59), Expect = 6.3
Identities = 26/95 (27%), Positives = 44/95 (46%)
Frame = +2
Query: 269 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFD 448
++I S MDV + + D ++DGYL + DL+E +G + T T+FD
Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKE-----EGKIKTVALTNFD 201
Query: 449 SGKELLCTVLKSCGEECVIAXKQTQLXTNKPTQHL 553
+ E L +L++ G V Q + +P Q +
Sbjct: 202 T--ERLQKILEN-GIPVVSNQVQHSIVDMRPQQRM 233
>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
contains INTERPRO domain, IPR001878: Zn-finger CCHC type
Length = 353
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%)
Frame = +1
Query: 70 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 195
KT T P PP PCN PC S + + R CP
Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 27.5 bits (58), Expect = 8.4
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +2
Query: 311 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 460
V+R+ +++ Y L ADN + + G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.5 bits (58), Expect = 8.4
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
Frame = +2
Query: 341 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 475
+SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At1g20860.1 68414.m02613 phosphate transporter family protein
similar to phosphate transporter [Catharanthus roseus]
GI:2208908, inorganic phosphate transporter 1 [Solanum
tuberosum] GI:1420871; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 534
Score = 27.5 bits (58), Expect = 8.4
Identities = 17/45 (37%), Positives = 20/45 (44%)
Frame = +1
Query: 46 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 180
K Q V S++H S T P PP P P K S C +H R
Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,661,804
Number of Sequences: 28952
Number of extensions: 301998
Number of successful extensions: 962
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 947
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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