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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060336.seq
         (685 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g02820.1 68418.m00224 DNA topoisomerase VIA (SPO11) identical...    30   1.6  
At1g66650.1 68414.m07573 seven in absentia (SINA) protein, putat...    29   2.2  
At4g23885.1 68417.m03435 expressed protein                             28   6.6  
At1g14950.1 68414.m01786 major latex protein-related / MLP-relat...    28   6.6  
At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative          27   8.8  

>At5g02820.1 68418.m00224 DNA topoisomerase VIA (SPO11) identical to
           topoisomerase 6 subunit A (spo11) [Arabidopsis thaliana]
           GI:12331186
          Length = 427

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 24/84 (28%), Positives = 41/84 (48%)
 Frame = +3

Query: 3   ARGGRTAAPRIFLFQLSRVKRKIQYYSAYNASSWGLLWLE*GVCGDACFLLDSTRFTSMD 182
           A+G    A R+FL ++ +++ K+   +  ++  +GL  L    CG      DS   T+ D
Sbjct: 280 AKGQPDVATRLFLRKM-KMELKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPD 338

Query: 183 DKRIRIARSELKRLVKGPLQRPIP 254
            K + I  S+L +  K P Q  +P
Sbjct: 339 IKWLGIRPSDLDK-YKIPEQCRLP 361


>At1g66650.1 68414.m07573 seven in absentia (SINA) protein, putative
           similar to SIAH2 protein [Brassica napus var. napus]
           GI:7657878; contains Pfam profile PF03145: Seven in
           absentia protein family
          Length = 329

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 1/70 (1%)
 Frame = +3

Query: 180 DDKRIR-IARSELKRLVKGPLQRPIPYQNRLLCNSIDLPIHNPTWLSFNGRSKNFNALLR 356
           DDK +  + R+  KR V  P    +P  N L C +   P+  P +   NG    F   ++
Sbjct: 54  DDKPLGDVLRTCRKRRVSSPKSVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIK 113

Query: 357 RHRLYSYCFL 386
             +  S+C L
Sbjct: 114 LKKRCSFCKL 123


>At4g23885.1 68417.m03435 expressed protein
          Length = 77

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 16/47 (34%), Positives = 22/47 (46%)
 Frame = -1

Query: 655 PAIKAQNLDYNPSDKQGKSMFAHFAKGWFCSQLSCLSSGSAVKVTGQ 515
           P IK      NPS  +GKS  A+ A   F S L+ +   +  K + Q
Sbjct: 11  PRIKFPQRHINPSASEGKSQVANEADTLFFSNLNNIQKTTGGKASSQ 57


>At1g14950.1 68414.m01786 major latex protein-related / MLP-related
           low similarity to major latex protein {Papaver
           somniferum}[GI:294060] ; contains Pfam profile PF00407:
           Pathogenesis-related protein Bet v I family
          Length = 155

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 15/36 (41%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
 Frame = +1

Query: 364 GCIATV-FFVMRAYGRSYSP-KYLKFIDTLDSPMDD 465
           GC+  V  F  + Y  S  P KY+KF+ +L + MDD
Sbjct: 111 GCVCKVTMFWEKRYEDSPEPIKYMKFVTSLAADMDD 146


>At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative 
          Length = 953

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 12/30 (40%), Positives = 17/30 (56%)
 Frame = -1

Query: 667 KVMGPAIKAQNLDYNPSDKQGKSMFAHFAK 578
           +V G     ++L +N  DK GKS+F H  K
Sbjct: 298 EVSGRDRTPEDLSHNAEDKAGKSIFLHLKK 327


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,844,408
Number of Sequences: 28952
Number of extensions: 335187
Number of successful extensions: 851
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 830
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 851
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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