BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060336.seq (685 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g02820.1 68418.m00224 DNA topoisomerase VIA (SPO11) identical... 30 1.6 At1g66650.1 68414.m07573 seven in absentia (SINA) protein, putat... 29 2.2 At4g23885.1 68417.m03435 expressed protein 28 6.6 At1g14950.1 68414.m01786 major latex protein-related / MLP-relat... 28 6.6 At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative 27 8.8 >At5g02820.1 68418.m00224 DNA topoisomerase VIA (SPO11) identical to topoisomerase 6 subunit A (spo11) [Arabidopsis thaliana] GI:12331186 Length = 427 Score = 29.9 bits (64), Expect = 1.6 Identities = 24/84 (28%), Positives = 41/84 (48%) Frame = +3 Query: 3 ARGGRTAAPRIFLFQLSRVKRKIQYYSAYNASSWGLLWLE*GVCGDACFLLDSTRFTSMD 182 A+G A R+FL ++ +++ K+ + ++ +GL L CG DS T+ D Sbjct: 280 AKGQPDVATRLFLRKM-KMELKLPVLALVDSDPYGLKILSVYGCGSKNMSYDSANLTTPD 338 Query: 183 DKRIRIARSELKRLVKGPLQRPIP 254 K + I S+L + K P Q +P Sbjct: 339 IKWLGIRPSDLDK-YKIPEQCRLP 361 >At1g66650.1 68414.m07573 seven in absentia (SINA) protein, putative similar to SIAH2 protein [Brassica napus var. napus] GI:7657878; contains Pfam profile PF03145: Seven in absentia protein family Length = 329 Score = 29.5 bits (63), Expect = 2.2 Identities = 20/70 (28%), Positives = 31/70 (44%), Gaps = 1/70 (1%) Frame = +3 Query: 180 DDKRIR-IARSELKRLVKGPLQRPIPYQNRLLCNSIDLPIHNPTWLSFNGRSKNFNALLR 356 DDK + + R+ KR V P +P N L C + P+ P + NG F ++ Sbjct: 54 DDKPLGDVLRTCRKRRVSSPKSVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIK 113 Query: 357 RHRLYSYCFL 386 + S+C L Sbjct: 114 LKKRCSFCKL 123 >At4g23885.1 68417.m03435 expressed protein Length = 77 Score = 27.9 bits (59), Expect = 6.6 Identities = 16/47 (34%), Positives = 22/47 (46%) Frame = -1 Query: 655 PAIKAQNLDYNPSDKQGKSMFAHFAKGWFCSQLSCLSSGSAVKVTGQ 515 P IK NPS +GKS A+ A F S L+ + + K + Q Sbjct: 11 PRIKFPQRHINPSASEGKSQVANEADTLFFSNLNNIQKTTGGKASSQ 57 >At1g14950.1 68414.m01786 major latex protein-related / MLP-related low similarity to major latex protein {Papaver somniferum}[GI:294060] ; contains Pfam profile PF00407: Pathogenesis-related protein Bet v I family Length = 155 Score = 27.9 bits (59), Expect = 6.6 Identities = 15/36 (41%), Positives = 21/36 (58%), Gaps = 2/36 (5%) Frame = +1 Query: 364 GCIATV-FFVMRAYGRSYSP-KYLKFIDTLDSPMDD 465 GC+ V F + Y S P KY+KF+ +L + MDD Sbjct: 111 GCVCKVTMFWEKRYEDSPEPIKYMKFVTSLAADMDD 146 >At2g01440.1 68415.m00067 ATP-dependent DNA helicase, putative Length = 953 Score = 27.5 bits (58), Expect = 8.8 Identities = 12/30 (40%), Positives = 17/30 (56%) Frame = -1 Query: 667 KVMGPAIKAQNLDYNPSDKQGKSMFAHFAK 578 +V G ++L +N DK GKS+F H K Sbjct: 298 EVSGRDRTPEDLSHNAEDKAGKSIFLHLKK 327 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,844,408 Number of Sequences: 28952 Number of extensions: 335187 Number of successful extensions: 851 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 830 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 851 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1447936096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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