BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060335.seq (665 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to p... 46 3e-05 At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to p... 43 2e-04 At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to S... 42 4e-04 At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identic... 42 5e-04 At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / aller... 40 0.002 At1g16900.1 68414.m02047 curculin-like (mannose-binding) lectin ... 27 8.5 >At2g19770.1 68415.m02310 profilin 4 (PRO4) (PFN4) identical to profilin 4 SP:Q38905 GI:1353768 from [Arabidopsis thaliana] Length = 134 Score = 45.6 bits (103), Expect = 3e-05 Identities = 22/43 (51%), Positives = 27/43 (62%) Frame = +1 Query: 325 GKVGVHCXKTQQAVVISLYEEPIQPQQXASVVEKLGXYLITCG 453 G G+ KT Q++V LYEEP+ P Q VVE+LG YLI G Sbjct: 91 GAGGITIKKTGQSMVFGLYEEPVTPGQCNMVVERLGDYLIEQG 133 Score = 40.7 bits (91), Expect = 9e-04 Identities = 21/42 (50%), Positives = 25/42 (59%), Gaps = 6/42 (14%) Frame = +3 Query: 81 MSWQDYVDKQLMAS------RCVTKAAIAGHDXNVWAKSEGF 188 MSWQ YVD+ LM +T AAI GHD +VWA+S F Sbjct: 1 MSWQAYVDEHLMCDVGDGQGHHLTAAAIIGHDGSVWAQSANF 42 >At4g29340.1 68417.m04192 profilin 3 (PRO3) (PFN3) identical to profilin 3 SP:Q38904 GI:1353765 from [Arabidopsis thaliana] Length = 134 Score = 43.2 bits (97), Expect = 2e-04 Identities = 20/43 (46%), Positives = 26/43 (60%) Frame = +1 Query: 325 GKVGVHCXKTQQAVVISLYEEPIQPQQXASVVEKLGXYLITCG 453 G G+ KT Q+ V +YEEP+ P Q VVE+LG YL+ G Sbjct: 91 GAGGITIKKTGQSCVFGIYEEPVTPGQCNMVVERLGDYLLEQG 133 Score = 41.5 bits (93), Expect = 5e-04 Identities = 21/42 (50%), Positives = 25/42 (59%), Gaps = 6/42 (14%) Frame = +3 Query: 81 MSWQDYVDKQLMAS------RCVTKAAIAGHDXNVWAKSEGF 188 MSWQ YVD+ LM +T AAI GHD +VWA+S F Sbjct: 1 MSWQTYVDEHLMCDVGDGQGHHLTAAAIVGHDGSVWAQSANF 42 >At5g56600.1 68418.m07065 profilin 5 (PRO5) (PRF3) identical to SP|Q9FE63 Profilin 5 {Arabidopsis thaliana} Length = 168 Score = 41.9 bits (94), Expect = 4e-04 Identities = 21/43 (48%), Positives = 25/43 (58%) Frame = +1 Query: 325 GKVGVHCXKTQQAVVISLYEEPIQPQQXASVVEKLGXYLITCG 453 G GV KT A+V +Y+EP+ P Q VVE LG YLI G Sbjct: 125 GAGGVTIKKTTLALVFGIYDEPMTPGQCNMVVENLGEYLIESG 167 Score = 41.5 bits (93), Expect = 5e-04 Identities = 21/39 (53%), Positives = 24/39 (61%), Gaps = 3/39 (7%) Frame = +3 Query: 81 MSWQDYVDKQLM---ASRCVTKAAIAGHDXNVWAKSEGF 188 MSWQ YVD LM A +T AAI G D +VWA+S F Sbjct: 38 MSWQTYVDDHLMCDVAGNRLTAAAILGQDGSVWAQSNNF 76 >At4g29350.1 68417.m04193 profilin 2 (PRO2) (PFN2) (PRF2) identical to profilin 2 SP:Q42418 GI:1353772 from [Arabidopsis thaliana]; identical to cDNA profilin (PRF2) GI:9965570 Length = 131 Score = 41.5 bits (93), Expect = 5e-04 Identities = 21/43 (48%), Positives = 26/43 (60%) Frame = +1 Query: 325 GKVGVHCXKTQQAVVISLYEEPIQPQQXASVVEKLGXYLITCG 453 G GV KT QA+V +Y+EP+ Q VVE+LG YLI G Sbjct: 88 GPGGVTIKKTTQALVFGIYDEPMTGGQCNLVVERLGDYLIESG 130 Score = 40.3 bits (90), Expect = 0.001 Identities = 24/55 (43%), Positives = 29/55 (52%), Gaps = 4/55 (7%) Frame = +3 Query: 81 MSWQDYVDKQLMAS---RCVTKAAIAGHDXNVWAKSEGF-XISKDXVAKIVAGFE 233 MSWQ YVD LM +T AAI G D +VWA+S F + +A I FE Sbjct: 1 MSWQSYVDDHLMCEVEGNHLTHAAIFGQDGSVWAQSSAFPQLKPAEIAGINKDFE 55 >At2g19760.1 68415.m02309 profilin 1 (PRO1) (PFN1) (PRF1) / allergen Ara t 8 identical to profilin 1 (Allergen Ara t 8) SP:Q42449 GI:1353770 from [Arabidopsis thaliana] Length = 131 Score = 39.5 bits (88), Expect = 0.002 Identities = 24/60 (40%), Positives = 29/60 (48%), Gaps = 4/60 (6%) Frame = +3 Query: 81 MSWQDYVDKQLMAS---RCVTKAAIAGHDXNVWAKSEGF-XISKDXVAKIVAGFENXSLL 248 MSWQ YVD LM +T AAI G D +VWA+S F + + I FE L Sbjct: 1 MSWQSYVDDHLMCDVEGNHLTAAAILGQDGSVWAQSAKFPQLKPQEIDGIKKDFEEPGFL 60 Score = 39.5 bits (88), Expect = 0.002 Identities = 20/40 (50%), Positives = 24/40 (60%) Frame = +1 Query: 325 GKVGVHCXKTQQAVVISLYEEPIQPQQXASVVEKLGXYLI 444 G GV KT QA+V Y+EP+ Q VVE+LG YLI Sbjct: 88 GPGGVTIKKTNQALVFGFYDEPMTGGQCNLVVERLGDYLI 127 >At1g16900.1 68414.m02047 curculin-like (mannose-binding) lectin family protein very low similarity to Ser Thr protein kinase GI:2598067 from (Zea mays); contains Pfam lectin (probable mannose binding) domain PF01453 Length = 919 Score = 27.5 bits (58), Expect = 8.5 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = -1 Query: 329 LPXSRG*YGLCX*GRCTACPL 267 LP G +G+C +C ACPL Sbjct: 279 LPSKCGAFGICEDNQCVACPL 299 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,893,060 Number of Sequences: 28952 Number of extensions: 193150 Number of successful extensions: 303 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 291 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 298 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1403159472 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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