Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060333.seq
(687 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 93 3e-20
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 93 3e-20
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 31 0.16
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 4.4
SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces p... 26 5.9
SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces pombe... 25 7.8
SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subuni... 25 7.8
SPBC9B6.06 |mrpl10||mitochondrial ribosomal protein subunit L15|... 25 7.8
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 93.1 bits (221), Expect = 3e-20
Identities = 42/59 (71%), Positives = 47/59 (79%)
Frame = +3
Query: 255 AGXQTSAESWGTGXAVARIPRVRGGGTHRSGXGAFGKMCRGGRMFRPQRSPGRRWHRRV 431
AG QTSAESWGTG A+ARIPRV GGGTHRSG AFG MCR GRMF P ++ R+WH +V
Sbjct: 58 AGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKT-WRKWHVKV 115
Score = 49.2 bits (112), Expect = 6e-07
Identities = 20/30 (66%), Positives = 23/30 (76%)
Frame = +2
Query: 158 PFVFKGPIRPXLVNDVHVSMSKNSRQPYCV 247
PFVFK PIRP LV VH +++KN RQPY V
Sbjct: 25 PFVFKAPIRPDLVRSVHTAVAKNKRQPYAV 54
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 93.1 bits (221), Expect = 3e-20
Identities = 42/59 (71%), Positives = 47/59 (79%)
Frame = +3
Query: 255 AGXQTSAESWGTGXAVARIPRVRGGGTHRSGXGAFGKMCRGGRMFRPQRSPGRRWHRRV 431
AG QTSAESWGTG A+ARIPRV GGGTHRSG AFG MCR GRMF P ++ R+WH +V
Sbjct: 58 AGHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKT-WRKWHVKV 115
Score = 49.2 bits (112), Expect = 6e-07
Identities = 20/30 (66%), Positives = 23/30 (76%)
Frame = +2
Query: 158 PFVFKGPIRPXLVNDVHVSMSKNSRQPYCV 247
PFVFK PIRP LV VH +++KN RQPY V
Sbjct: 25 PFVFKAPIRPDLVRSVHTAVAKNKRQPYAV 54
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 31.1 bits (67), Expect = 0.16
Identities = 28/102 (27%), Positives = 46/102 (45%), Gaps = 4/102 (3%)
Frame = +3
Query: 72 RNESIGSPTFSVGVF--RKERDGAGCSQAPSRSCSR-GPYVRXWSMMFTF-LCPRTRGSP 239
R+ ++G +F+ VF R+ RD A SR G V S+ + + PR R +P
Sbjct: 325 RDRAVGVASFNPEVFQTRRRRDLAAAESNDDAQASRTGRTVIPRSIDSRYQVAPRVRLNP 384
Query: 240 TA*XGAGXQTSAESWGTGXAVARIPRVRGGGTHRSGXGAFGK 365
TA AG A++ A++ +R + +SG G+
Sbjct: 385 TAMTIAGRMKPADTEQVRKLKAKMKAIRSKSSKKSGVSIEGR 426
>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 606
Score = 26.2 bits (55), Expect = 4.4
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = -2
Query: 143 APCTVSLFSEYTDTKGRATDRLISLL 66
A C V ++SEYT G ++ L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515
>SPAC2F7.17 |||peptide chain release factor|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 396
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = +3
Query: 345 GXGAFGKMCRGGRMFRPQRSPGRRWHRRVKXPTA 446
G GA+G + G + R QR+P RV TA
Sbjct: 202 GEGAYGHLMLEGGVHRVQRTPATETKGRVHTSTA 235
>SPAC644.11c |||pyruvate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 25.4 bits (53), Expect = 7.8
Identities = 16/47 (34%), Positives = 19/47 (40%), Gaps = 1/47 (2%)
Frame = +3
Query: 192 WSMMFTFLCPRTRGSPTA*XGAGXQTSAESWGTGXAVARI-PRVRGG 329
WS MFT P P A T +G G +AR+ R GG
Sbjct: 350 WSYMFTTASPTLTDDPHDIVSANSTTPMAGFGFGLPLARLYTRYFGG 396
>SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subunit
8|Schizosaccharomyces pombe|chr 3|||Manual
Length = 230
Score = 25.4 bits (53), Expect = 7.8
Identities = 16/52 (30%), Positives = 24/52 (46%), Gaps = 3/52 (5%)
Frame = +3
Query: 531 EKE-FPETFPWVVSRXKFQEXQTRPKQGVHLS*RRLXRHWSDNPL--RWYKV 677
+KE F E F V K + T + VH RR + W+D + +WY +
Sbjct: 12 QKEMFNEIFKEYVKEEKQIDLGTCDQDSVHDLWRRFVKRWNDGKMKSKWYSL 63
>SPBC9B6.06 |mrpl10||mitochondrial ribosomal protein subunit
L15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 220
Score = 25.4 bits (53), Expect = 7.8
Identities = 11/28 (39%), Positives = 16/28 (57%)
Frame = -3
Query: 460 KGPLSAVGXLTRRCQRRPGLRWGRNIRP 377
+GP S +G + R + G R GR I+P
Sbjct: 19 RGPASGLGKTSGRGHKGSGQRRGRRIKP 46
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,731,930
Number of Sequences: 5004
Number of extensions: 53907
Number of successful extensions: 118
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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