BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060323.seq (694 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2... 137 2e-33 SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosa... 130 2e-31 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 31 0.16 SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 28 1.1 SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr... 27 3.4 SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pomb... 26 5.9 >SPBC1815.01 |eno101|eno1|enolase|Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 137 bits (331), Expect = 2e-33 Identities = 70/146 (47%), Positives = 91/146 (62%), Gaps = 1/146 (0%) Frame = +1 Query: 256 IFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNIN 435 I+DSRGNPTVEVDL TE G+ RA VPSGASTG+ EALE+RD K+++ GKGVL A+ N+N Sbjct: 11 IYDSRGNPTVEVDLTTETGIHRAIVPSGASTGIWEALEMRDGDKTKWGGKGVLKAVGNVN 70 Query: 436 ELIAPELTKANLEVTQQREIDELMLSWMALRTNPNWVXXXXXXXXXXXXXXXXPRKMFPL 615 +IAP + KANL+VT Q+ DE +L +K PL Sbjct: 71 NIIAPAVVKANLDVTDQKAADEFLLKLDGTENKSKLGANAILGVSMAICRAGAAQKKLPL 130 Query: 616 YKHLADLAG-NNDIVLPVPAFNVING 690 +K++A+ G VLPVP+FNV+NG Sbjct: 131 WKYIAENFGTKGPYVLPVPSFNVLNG 156 >SPBPB21E7.01c |eno102|eno1, SPBPB8B6.07c, eno1|enolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 440 Score = 130 bits (314), Expect = 2e-31 Identities = 67/147 (45%), Positives = 89/147 (60%), Gaps = 1/147 (0%) Frame = +1 Query: 253 SIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNI 432 SI+DSRGNPTVEV+L TELG FR+ VPSGASTG EA ELRDN K+++ GKGV A+ N+ Sbjct: 11 SIYDSRGNPTVEVELTTELGTFRSMVPSGASTGEWEAKELRDNDKNKWGGKGVTIAVHNV 70 Query: 433 NELIAPELTKANLEVTQQREIDELMLSWMALRTNPNWVXXXXXXXXXXXXXXXXPRKMFP 612 N +I P L K+++++T QR IDE M+ P Sbjct: 71 NNIIGPALVKSDIKITDQRGIDEFMIKLDGTNDKSKLGANSIVGVSMAVARAAAAFLKIP 130 Query: 613 LYKHLADLAGNNDI-VLPVPAFNVING 690 LY+++ LAG+ +PVP+FNV+NG Sbjct: 131 LYEYIGKLAGSKTTECIPVPSFNVLNG 157 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 31.1 bits (67), Expect = 0.16 Identities = 18/40 (45%), Positives = 19/40 (47%) Frame = +1 Query: 232 NKINQGSSIFDSRGNPTVEVDLVTELGLFRAAVPSGASTG 351 N NQ +S F PTV VDL L RA P STG Sbjct: 519 NSGNQSTSSFSRYNQPTVSVDLRNSSILRRAQEPVNGSTG 558 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 28.3 bits (60), Expect = 1.1 Identities = 22/72 (30%), Positives = 32/72 (44%) Frame = +1 Query: 262 DSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINEL 441 D +G +VD ++A +ST LE R N + E V TAI + E Sbjct: 140 DEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDD-AVHTAILALKET 198 Query: 442 IAPELTKANLEV 477 ELT+ N+E+ Sbjct: 199 FEGELTEDNIEI 210 >SPCC1672.03c |||guanine deaminase |Schizosaccharomyces pombe|chr 3|||Manual Length = 527 Score = 26.6 bits (56), Expect = 3.4 Identities = 9/15 (60%), Positives = 11/15 (73%) Frame = +2 Query: 569 PSCC*GWCCQEKCFR 613 PSCC G CC+E+ R Sbjct: 490 PSCCGGHCCKEESCR 504 >SPCC290.03c |nup186||nucleoporin Nup186|Schizosaccharomyces pombe|chr 3|||Manual Length = 1647 Score = 25.8 bits (54), Expect = 5.9 Identities = 18/77 (23%), Positives = 34/77 (44%) Frame = +1 Query: 271 GNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNINELIAP 450 G P + ++ LG F + S S + ++ + + +E H + K +NE +A Sbjct: 1109 GEPILTQQVIQNLGGFSSEEVSMVSRCIRSRTQIMNMLATEIHYAASVGQNKYLNEYVA- 1167 Query: 451 ELTKANLEVTQQREIDE 501 L + N E T E+ + Sbjct: 1168 SLIRTN-EKTHSTELSQ 1183 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,498,181 Number of Sequences: 5004 Number of extensions: 43234 Number of successful extensions: 113 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 105 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 109 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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