BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060323.seq (694 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot... 140 6e-34 At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ... 120 7e-28 At2g29560.1 68415.m03590 enolase, putative similar to enolase [S... 110 7e-25 At3g55110.1 68416.m06120 ABC transporter family protein ATP-bind... 33 0.24 At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger) fa... 29 2.2 At1g23560.1 68414.m02964 expressed protein contains Pfam profile... 29 2.9 At1g64050.1 68414.m07255 expressed protein 28 5.1 At3g25500.1 68416.m03171 formin homology 2 domain-containing pro... 28 6.8 >At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot:P15007 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Drosophila melanogaster] Length = 477 Score = 140 bits (340), Expect = 6e-34 Identities = 75/145 (51%), Positives = 93/145 (64%) Frame = +1 Query: 256 IFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNIN 435 I DSRGNPTVEVDL+T+ L+R+AVPSGASTG++EALELRD KS Y GKGVL AIKNIN Sbjct: 59 IIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIKNIN 117 Query: 436 ELIAPELTKANLEVTQQREIDELMLSWMALRTNPNWVXXXXXXXXXXXXXXXXPRKMFPL 615 EL+AP+L ++V Q ++D LML K PL Sbjct: 118 ELVAPKL--IGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPL 175 Query: 616 YKHLADLAGNNDIVLPVPAFNVING 690 YKH+ + +G ++V+PVPAFNVING Sbjct: 176 YKHIQETSGTKELVMPVPAFNVING 200 >At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Arabidopsis thaliana] Length = 444 Score = 120 bits (290), Expect = 7e-28 Identities = 75/152 (49%), Positives = 89/152 (58%), Gaps = 7/152 (4%) Frame = +1 Query: 256 IFDSRGNPTVEVDLVTELGL-FRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNI 432 IFDSRGNPTVEVD+ T G+ AAVPSGASTG++EALELRD S+Y GKGV A+ N+ Sbjct: 12 IFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDG-GSDYLGKGVSKAVGNV 70 Query: 433 NELIAPELTKANLEVTQQREIDELMLSWMALRTNP-NWVXXXXXXXXXXXXXXXXPR--- 600 N +I P L + TQQ ID M+ + N W + Sbjct: 71 NNIIGPAL--IGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCKAGA 128 Query: 601 --KMFPLYKHLADLAGNNDIVLPVPAFNVING 690 PLYKH+A+LAGN IVLPVPAFNVING Sbjct: 129 VVSGIPLYKHIANLAGNPKIVLPVPAFNVING 160 >At2g29560.1 68415.m03590 enolase, putative similar to enolase [Spinacia oleracea] gi|8919731|emb|CAB96173 Length = 475 Score = 110 bits (265), Expect = 7e-25 Identities = 63/145 (43%), Positives = 81/145 (55%) Frame = +1 Query: 256 IFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNIN 435 I DSRG PTVEVDL T G+FRA+VPSG S+G +EA+ELRD K Y G V A+KNIN Sbjct: 55 ILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNIN 114 Query: 436 ELIAPELTKANLEVTQQREIDELMLSWMALRTNPNWVXXXXXXXXXXXXXXXXPRKMFPL 615 E I+ L ++ Q +ID+ M+ K PL Sbjct: 115 EKISEAL--IGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPL 172 Query: 616 YKHLADLAGNNDIVLPVPAFNVING 690 KHL+DL+G ++VLPVPAF V++G Sbjct: 173 CKHLSDLSGRANMVLPVPAFTVLSG 197 >At3g55110.1 68416.m06120 ABC transporter family protein ATP-binding cassette-sub-family G-member 2, Mus musculus, EMBL:AF140218 Length = 708 Score = 32.7 bits (71), Expect = 0.24 Identities = 20/85 (23%), Positives = 38/85 (44%) Frame = +1 Query: 217 SIKNGNKINQGSSIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEY 396 S+ NG+ ++ S F S G P E + +TE L G+S G + +E + + Sbjct: 295 SVFNGSPVSL-PSFFSSFGRPIPEKENITEFALDVIRELEGSSEGTRDLVEFNEKWQQNQ 353 Query: 397 HGKGVLTAIKNINELIAPELTKANL 471 + + ++ E IA +++ L Sbjct: 354 TARATTQSRVSLKEAIAASVSRGKL 378 >At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger) family protein contains a Zinc finger, C3HC4 type (RING finger) signature, PROSITE:PS00518 Length = 688 Score = 29.5 bits (63), Expect = 2.2 Identities = 18/53 (33%), Positives = 32/53 (60%), Gaps = 3/53 (5%) Frame = +1 Query: 358 EALELRDNIKSEYHGKG--VLTAIKNINELIA-PELTKANLEVTQQREIDELM 507 E+ E+ D + EY K +LT +K++ ++ PELT+ L++ Q + DEL+ Sbjct: 143 ESEEIADGV-DEYETKEDIMLTILKDLRSSVSEPELTEEQLKMNDQLQEDELL 194 >At1g23560.1 68414.m02964 expressed protein contains Pfam profile PF02713: Domain of unknown function DUF220 Length = 332 Score = 29.1 bits (62), Expect = 2.9 Identities = 12/48 (25%), Positives = 23/48 (47%) Frame = +1 Query: 403 KGVLTAIKNINELIAPELTKANLEVTQQREIDELMLSWMALRTNPNWV 546 K ++N+ EL ++K + R+ ++L A R NP+W+ Sbjct: 43 KAEFKRLRNVKELSLKSVSKIETAYEEHRDEEKLEKQLQAWRDNPSWI 90 >At1g64050.1 68414.m07255 expressed protein Length = 668 Score = 28.3 bits (60), Expect = 5.1 Identities = 11/33 (33%), Positives = 20/33 (60%) Frame = -3 Query: 353 TPVEAPEGTAARNKPSSVTRSTSTVGLPRESKI 255 +P+ +PE + K ++RS+S G PR +K+ Sbjct: 107 SPIASPESAESPRKRGKLSRSSSGNGTPRRTKL 139 >At3g25500.1 68416.m03171 formin homology 2 domain-containing protein / FH2 domain-containing protein contains formin homology 2 domain, Pfam:PF02181 Length = 1051 Score = 27.9 bits (59), Expect = 6.8 Identities = 16/49 (32%), Positives = 26/49 (53%) Frame = +2 Query: 161 YPQSIQEIFLQYPXKSISSVLKMVIKSIKARQSLTLVATLQWKLIW*QS 307 +P +I + + + KS + K++I +I A S LVA L L W +S Sbjct: 86 FPANISSLIVPHATKSPPNSKKLLIVAISAVSSAALVALLIALLYWRRS 134 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,882,549 Number of Sequences: 28952 Number of extensions: 223775 Number of successful extensions: 566 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 549 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 559 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1477286152 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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