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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060323.seq
         (694 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot...   140   6e-34
At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696 ...   120   7e-28
At2g29560.1 68415.m03590 enolase, putative similar to enolase [S...   110   7e-25
At3g55110.1 68416.m06120 ABC transporter family protein ATP-bind...    33   0.24 
At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger) fa...    29   2.2  
At1g23560.1 68414.m02964 expressed protein contains Pfam profile...    29   2.9  
At1g64050.1 68414.m07255 expressed protein                             28   5.1  
At3g25500.1 68416.m03171 formin homology 2 domain-containing pro...    28   6.8  

>At1g74030.1 68414.m08573 enolase, putative similar to
           Swiss-Prot:P15007 enolase (EC 4.2.1.11)
           (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
           hydro-lyase) [Drosophila melanogaster]
          Length = 477

 Score =  140 bits (340), Expect = 6e-34
 Identities = 75/145 (51%), Positives = 93/145 (64%)
 Frame = +1

Query: 256 IFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNIN 435
           I DSRGNPTVEVDL+T+  L+R+AVPSGASTG++EALELRD  KS Y GKGVL AIKNIN
Sbjct: 59  IIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQAIKNIN 117

Query: 436 ELIAPELTKANLEVTQQREIDELMLSWMALRTNPNWVXXXXXXXXXXXXXXXXPRKMFPL 615
           EL+AP+L    ++V  Q ++D LML                              K  PL
Sbjct: 118 ELVAPKL--IGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPL 175

Query: 616 YKHLADLAGNNDIVLPVPAFNVING 690
           YKH+ + +G  ++V+PVPAFNVING
Sbjct: 176 YKHIQETSGTKELVMPVPAFNVING 200


>At2g36530.1 68415.m04481 enolase identical to SWISS-PROT:P25696
           enolase (EC 4.2.1.11) (2-phosphoglycerate
           dehydratase)(2-phospho-D- glycerate hydro-lyase)
           [Arabidopsis thaliana]
          Length = 444

 Score =  120 bits (290), Expect = 7e-28
 Identities = 75/152 (49%), Positives = 89/152 (58%), Gaps = 7/152 (4%)
 Frame = +1

Query: 256 IFDSRGNPTVEVDLVTELGL-FRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNI 432
           IFDSRGNPTVEVD+ T  G+   AAVPSGASTG++EALELRD   S+Y GKGV  A+ N+
Sbjct: 12  IFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDG-GSDYLGKGVSKAVGNV 70

Query: 433 NELIAPELTKANLEVTQQREIDELMLSWMALRTNP-NWVXXXXXXXXXXXXXXXXPR--- 600
           N +I P L     + TQQ  ID  M+  +    N   W                  +   
Sbjct: 71  NNIIGPAL--IGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLAVCKAGA 128

Query: 601 --KMFPLYKHLADLAGNNDIVLPVPAFNVING 690
                PLYKH+A+LAGN  IVLPVPAFNVING
Sbjct: 129 VVSGIPLYKHIANLAGNPKIVLPVPAFNVING 160


>At2g29560.1 68415.m03590 enolase, putative similar to enolase
           [Spinacia oleracea] gi|8919731|emb|CAB96173
          Length = 475

 Score =  110 bits (265), Expect = 7e-25
 Identities = 63/145 (43%), Positives = 81/145 (55%)
 Frame = +1

Query: 256 IFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEYHGKGVLTAIKNIN 435
           I DSRG PTVEVDL T  G+FRA+VPSG S+G +EA+ELRD  K  Y G  V  A+KNIN
Sbjct: 55  ILDSRGIPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNIN 114

Query: 436 ELIAPELTKANLEVTQQREIDELMLSWMALRTNPNWVXXXXXXXXXXXXXXXXPRKMFPL 615
           E I+  L    ++   Q +ID+ M+                              K  PL
Sbjct: 115 EKISEAL--IGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPL 172

Query: 616 YKHLADLAGNNDIVLPVPAFNVING 690
            KHL+DL+G  ++VLPVPAF V++G
Sbjct: 173 CKHLSDLSGRANMVLPVPAFTVLSG 197


>At3g55110.1 68416.m06120 ABC transporter family protein ATP-binding
           cassette-sub-family G-member 2, Mus musculus,
           EMBL:AF140218
          Length = 708

 Score = 32.7 bits (71), Expect = 0.24
 Identities = 20/85 (23%), Positives = 38/85 (44%)
 Frame = +1

Query: 217 SIKNGNKINQGSSIFDSRGNPTVEVDLVTELGLFRAAVPSGASTGVHEALELRDNIKSEY 396
           S+ NG+ ++   S F S G P  E + +TE  L       G+S G  + +E  +  +   
Sbjct: 295 SVFNGSPVSL-PSFFSSFGRPIPEKENITEFALDVIRELEGSSEGTRDLVEFNEKWQQNQ 353

Query: 397 HGKGVLTAIKNINELIAPELTKANL 471
             +    +  ++ E IA  +++  L
Sbjct: 354 TARATTQSRVSLKEAIAASVSRGKL 378


>At1g32340.1 68414.m03985 zinc finger (C3HC4-type RING finger)
           family protein contains a Zinc finger, C3HC4 type (RING
           finger) signature, PROSITE:PS00518
          Length = 688

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 18/53 (33%), Positives = 32/53 (60%), Gaps = 3/53 (5%)
 Frame = +1

Query: 358 EALELRDNIKSEYHGKG--VLTAIKNINELIA-PELTKANLEVTQQREIDELM 507
           E+ E+ D +  EY  K   +LT +K++   ++ PELT+  L++  Q + DEL+
Sbjct: 143 ESEEIADGV-DEYETKEDIMLTILKDLRSSVSEPELTEEQLKMNDQLQEDELL 194


>At1g23560.1 68414.m02964 expressed protein contains Pfam profile
           PF02713: Domain of unknown function DUF220
          Length = 332

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 12/48 (25%), Positives = 23/48 (47%)
 Frame = +1

Query: 403 KGVLTAIKNINELIAPELTKANLEVTQQREIDELMLSWMALRTNPNWV 546
           K     ++N+ EL    ++K      + R+ ++L     A R NP+W+
Sbjct: 43  KAEFKRLRNVKELSLKSVSKIETAYEEHRDEEKLEKQLQAWRDNPSWI 90


>At1g64050.1 68414.m07255 expressed protein
          Length = 668

 Score = 28.3 bits (60), Expect = 5.1
 Identities = 11/33 (33%), Positives = 20/33 (60%)
 Frame = -3

Query: 353 TPVEAPEGTAARNKPSSVTRSTSTVGLPRESKI 255
           +P+ +PE   +  K   ++RS+S  G PR +K+
Sbjct: 107 SPIASPESAESPRKRGKLSRSSSGNGTPRRTKL 139


>At3g25500.1 68416.m03171 formin homology 2 domain-containing
           protein / FH2 domain-containing protein contains formin
           homology 2 domain, Pfam:PF02181
          Length = 1051

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 16/49 (32%), Positives = 26/49 (53%)
 Frame = +2

Query: 161 YPQSIQEIFLQYPXKSISSVLKMVIKSIKARQSLTLVATLQWKLIW*QS 307
           +P +I  + + +  KS  +  K++I +I A  S  LVA L   L W +S
Sbjct: 86  FPANISSLIVPHATKSPPNSKKLLIVAISAVSSAALVALLIALLYWRRS 134


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,882,549
Number of Sequences: 28952
Number of extensions: 223775
Number of successful extensions: 566
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 549
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 559
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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