BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060320.seq (658 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 122 3e-29 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 122 3e-29 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 93 2e-20 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 60 2e-10 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 53 3e-08 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 50 2e-07 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 40 2e-04 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 31 0.11 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 31 0.11 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 31 0.11 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 30 0.26 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 27 2.4 SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 7.3 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 7.3 SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma su... 25 9.6 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 122 bits (295), Expect = 3e-29 Identities = 67/134 (50%), Positives = 87/134 (64%), Gaps = 1/134 (0%) Frame = +1 Query: 259 RCITIKSTAISMFFELEEKXLVFITNPDQREKSEXX-FLINLIDSXGHVDFSSEVTAALR 435 R +TIKSTAIS+F E+ + + D +E ++ FL+NLIDS GHVDFSSEVTAALR Sbjct: 66 RGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALR 120 Query: 436 VTDGALXXXXXXXXXXXQTETVLRRAIAXRIKPILFMNKMDRXXXXXXXXXXXXYQTXQR 615 VTDGAL QTETVLR+A+ RI+P++ +NK+DR YQ R Sbjct: 121 VTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFAR 180 Query: 616 IVENVNVLIATYND 657 +VE+VNV+I+TY D Sbjct: 181 VVESVNVVISTYYD 194 Score = 68.1 bits (159), Expect = 1e-12 Identities = 31/42 (73%), Positives = 35/42 (83%) Frame = +2 Query: 65 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 190 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV K Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQK 42 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 122 bits (295), Expect = 3e-29 Identities = 67/134 (50%), Positives = 87/134 (64%), Gaps = 1/134 (0%) Frame = +1 Query: 259 RCITIKSTAISMFFELEEKXLVFITNPDQREKSEXX-FLINLIDSXGHVDFSSEVTAALR 435 R +TIKSTAIS+F E+ + + D +E ++ FL+NLIDS GHVDFSSEVTAALR Sbjct: 66 RGVTIKSTAISLFAEMTDDDM-----KDMKEPADGTDFLVNLIDSPGHVDFSSEVTAALR 120 Query: 436 VTDGALXXXXXXXXXXXQTETVLRRAIAXRIKPILFMNKMDRXXXXXXXXXXXXYQTXQR 615 VTDGAL QTETVLR+A+ RI+P++ +NK+DR YQ R Sbjct: 121 VTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRALLELQISQEELYQNFAR 180 Query: 616 IVENVNVLIATYND 657 +VE+VNV+I+TY D Sbjct: 181 VVESVNVVISTYYD 194 Score = 68.1 bits (159), Expect = 1e-12 Identities = 31/42 (73%), Positives = 35/42 (83%) Frame = +2 Query: 65 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 190 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV K Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQK 42 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 93.5 bits (222), Expect = 2e-20 Identities = 58/131 (44%), Positives = 77/131 (58%) Frame = +1 Query: 259 RCITIKSTAISMFFELEEKXLVFITNPDQREKSEXXFLINLIDSXGHVDFSSEVTAALRV 438 R IT+KS+AIS+FF++ I+ D++ + E +LINLIDS GHVDFSSEV++A R+ Sbjct: 66 RGITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVDFSSEVSSASRL 117 Query: 439 TDGALXXXXXXXXXXXQTETVLRRAIAXRIKPILFMNKMDRXXXXXXXXXXXXYQTXQRI 618 DGA QT TVLR+A RIK IL +NKMDR + R+ Sbjct: 118 CDGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDRLITELKLSPIEAHYHLLRL 177 Query: 619 VENVNVLIATY 651 VE VN +I T+ Sbjct: 178 VEQVNAVIGTF 188 Score = 42.7 bits (96), Expect = 4e-05 Identities = 16/35 (45%), Positives = 27/35 (77%) Frame = +2 Query: 83 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVS 187 +++ + + NIRN +++AHVDHGK+TL DSL++ Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLA 41 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 60.5 bits (140), Expect = 2e-10 Identities = 38/99 (38%), Positives = 51/99 (51%) Frame = +1 Query: 265 ITIKSTAISMFFELEEKXLVFITNPDQREKSEXXFLINLIDSXGHVDFSSEVTAALRVTD 444 ITI+S A +E + N Q+ E + IN+ID+ GH+DF+ EV ALRV D Sbjct: 113 ITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERALRVLD 170 Query: 445 GALXXXXXXXXXXXQTETVLRRAIAXRIKPILFMNKMDR 561 GA+ QT TV R+ + I F+NKMDR Sbjct: 171 GAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDR 209 Score = 33.9 bits (74), Expect = 0.021 Identities = 16/40 (40%), Positives = 24/40 (60%), Gaps = 2/40 (5%) Frame = +2 Query: 71 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLV 184 N + E DKKR IRN+ + AH+D GK+T T+ ++ Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVL 80 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 53.2 bits (122), Expect = 3e-08 Identities = 26/63 (41%), Positives = 37/63 (58%) Frame = +1 Query: 373 INLIDSXGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRRAIAXRIKPILFMNK 552 INLID+ GH DF+ EV ++ V DGA+ QT+ V ++A I ++F+NK Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAGVEAQTKVVWKQATKRGIPKVIFVNK 154 Query: 553 MDR 561 MDR Sbjct: 155 MDR 157 Score = 33.9 bits (74), Expect = 0.021 Identities = 12/23 (52%), Positives = 20/23 (86%) Frame = +2 Query: 116 NIRNMSVIAHVDHGKSTLTDSLV 184 +IRN+ +IAH+D GK+TLT+ ++ Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKML 49 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 50.4 bits (115), Expect = 2e-07 Identities = 24/89 (26%), Positives = 40/89 (44%) Frame = +1 Query: 367 FLINLIDSXGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRRAIAXRIKPILFM 546 F ID+ GHVDF EV A + ++DG + T +++ AI + +L + Sbjct: 209 FAFQCIDTPGHVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHAILHDMPIVLVL 268 Query: 547 NKMDRXXXXXXXXXXXXYQTXQRIVENVN 633 NK+DR Y + +++ VN Sbjct: 269 NKVDRLILELRLPPNDAYHKLRHVIDEVN 297 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 40.3 bits (90), Expect = 2e-04 Identities = 21/64 (32%), Positives = 33/64 (51%) Frame = +1 Query: 367 FLINLIDSXGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRRAIAXRIKPILFM 546 +L+NLID+ GHVDF +EV +L +G + QT + A + + I + Sbjct: 124 YLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQGIQAQTLSNFYMAFSQNLVIIPVL 183 Query: 547 NKMD 558 NK+D Sbjct: 184 NKVD 187 Score = 38.7 bits (86), Expect = 7e-04 Identities = 16/32 (50%), Positives = 25/32 (78%) Frame = +2 Query: 89 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLV 184 +RG+ + +RN +VIAH+DHGKSTL+D ++ Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCIL 79 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 31.5 bits (68), Expect = 0.11 Identities = 15/30 (50%), Positives = 19/30 (63%) Frame = +2 Query: 101 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 190 M K++ N+ VI HVD GKST T L+ K Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYK 30 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 31.5 bits (68), Expect = 0.11 Identities = 15/30 (50%), Positives = 19/30 (63%) Frame = +2 Query: 101 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 190 M K++ N+ VI HVD GKST T L+ K Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYK 30 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 31.5 bits (68), Expect = 0.11 Identities = 15/30 (50%), Positives = 19/30 (63%) Frame = +2 Query: 101 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSK 190 M K++ N+ VI HVD GKST T L+ K Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYK 30 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 30.3 bits (65), Expect = 0.26 Identities = 13/25 (52%), Positives = 19/25 (76%) Frame = +2 Query: 107 KKRNIRNMSVIAHVDHGKSTLTDSL 181 KK ++ N+ I HVDHGK+TLT ++ Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAI 73 Score = 27.5 bits (58), Expect = 1.8 Identities = 16/60 (26%), Positives = 25/60 (41%), Gaps = 1/60 (1%) Frame = +1 Query: 382 IDSXGHVDFSSEVTAALRVTDGALXXXXXXXXXXXQTETVLRRAIAXRIKPI-LFMNKMD 558 +D GH D+ + DGA+ QT L A +K I +++NK+D Sbjct: 121 VDCPGHADYIKNMITGAATMDGAIIVVSATDGQMPQTREHLLLARQVGVKQIVVYINKVD 180 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 27.1 bits (57), Expect = 2.4 Identities = 9/17 (52%), Positives = 15/17 (88%) Frame = +2 Query: 128 MSVIAHVDHGKSTLTDS 178 ++++ HVDHGK+TL D+ Sbjct: 174 VTLMGHVDHGKTTLLDA 190 >SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.4 bits (53), Expect = 7.3 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -1 Query: 121 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 26 D P + +H D ++E H RW + +LLN++ Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 7.3 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -3 Query: 116 SASCPSSHGSRLP*NSPF*MVYDFCSIK 33 +ASCP SH L + PF + + C IK Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444 >SPBC17G9.09 |tif213||translation initiation factor eIF2 gamma subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 446 Score = 25.0 bits (52), Expect = 9.6 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +2 Query: 125 NMSVIAHVDHGKSTLTDSL 181 N+ I HV HGKST+ ++ Sbjct: 25 NIGTIGHVAHGKSTVVKAI 43 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,290,914 Number of Sequences: 5004 Number of extensions: 40773 Number of successful extensions: 98 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 86 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 95 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 297805304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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