BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060314.seq (685 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g02800.1 68414.m00237 endo-1,4-beta-glucanase / cellulase (CE... 31 0.94 At5g20200.1 68418.m02406 nucleoporin-related contains weak simil... 30 1.2 At5g56560.1 68418.m07058 F-box family protein contains F-box dom... 29 2.2 At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 29 2.2 At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 29 3.8 At5g50060.1 68418.m06199 invertase/pectin methylesterase inhibit... 28 6.6 At4g24240.1 68417.m03479 WRKY family transcription factor contai... 28 6.6 At3g22060.1 68416.m02784 receptor protein kinase-related contain... 27 8.8 At1g11590.1 68414.m01330 pectin methylesterase, putative similar... 27 8.8 >At1g02800.1 68414.m00237 endo-1,4-beta-glucanase / cellulase (CEL2) identical to endo-1,4-beta glucanase; ATCEL2 GI:3132891 from [Arabidopsis thaliana] Length = 501 Score = 30.7 bits (66), Expect = 0.94 Identities = 26/76 (34%), Positives = 36/76 (47%) Frame = -1 Query: 676 SISRCSCHQFVMLCSHSSRDILCWSVTL*GSKRSLARNPGPVQSGIVFSVSGLDRVEASN 497 S SR S H L +H+ +D L S+ +RS + P P Q S SGL A N Sbjct: 27 SSSRPSIHHRHHLDNHNYKDALSKSILFFEGQRS-GKLP-PNQRMTWRSNSGLSDGSALN 84 Query: 496 TEVSGGQYISSDKIAF 449 ++ GG Y + D + F Sbjct: 85 VDLVGGYYDAGDNMKF 100 >At5g20200.1 68418.m02406 nucleoporin-related contains weak similarity to Nucleoporin NUP1 (Nuclear pore protein NUP1) (Swiss-Prot:P20676) [Saccharomyces cerevisiae] Length = 762 Score = 30.3 bits (65), Expect = 1.2 Identities = 12/29 (41%), Positives = 20/29 (68%) Frame = +1 Query: 214 LFDQVVKTIGLREVGSSVFSTPTPRATSL 300 LFD+ + IG ++ S +++TPTP A S+ Sbjct: 213 LFDKAKEPIGGKDANSEIWATPTPLAKSI 241 >At5g56560.1 68418.m07058 F-box family protein contains F-box domain Pfam:PF00646 Length = 607 Score = 29.5 bits (63), Expect = 2.2 Identities = 14/50 (28%), Positives = 28/50 (56%) Frame = +1 Query: 97 VCVIITIKMVAGGKQMNVRVTTMDAELEFAIQQTTTGKQLFDQVVKTIGL 246 +C ++ +V G K +NV++ MD ++ +TG+ + + KT+GL Sbjct: 175 ICPVLERLVVDGTKGVNVKIPNMDVPNLRSLSFRSTGELRIELLRKTLGL 224 >At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2 (CPK2) identical to calcium-dependent protein kinase isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535; contains protein kinase domain, Pfam:PF00069; contains EF hand domain (calcium-binding EF-hand), Pfam:PF00036, INTERPRO:IPR002048 Length = 646 Score = 29.5 bits (63), Expect = 2.2 Identities = 18/70 (25%), Positives = 36/70 (51%) Frame = +3 Query: 324 MQQDVKKENPLQFKFRAKFYPEDVDDELIQEITLKLFYLQVKNAILSDEIYCPPETSVLL 503 +Q + KKE L + + K PE+ E+ E + + +Q ++A + PET+ Sbjct: 83 IQPEEKKEKVLAEETKQKVVPEESKQEVPPEESKREVVVQPESAKPETKSESKPETTKPE 142 Query: 504 ASTRSKPDTE 533 ++ +KP+T+ Sbjct: 143 TTSETKPETK 152 >At4g30160.1 68417.m04289 villin, putative similar to villin 2 (VLN2) [Arabidopsis thaliana] GI:3415115, villin 3 (VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam profiles PF00626: Gelsolin repeat, PF02209: Villin headpiece domain Length = 974 Score = 28.7 bits (61), Expect = 3.8 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Frame = -2 Query: 636 APIPRGTSCVGRS-LFEEVNGHWPGIPAPCRAGLCSP 529 AP P+ ++ RS LFE++ P IP P +A +P Sbjct: 828 APAPKSSAIASRSALFEKIPPQEPSIPKPVKASPKTP 864 >At5g50060.1 68418.m06199 invertase/pectin methylesterase inhibitor family protein low similarity to pollen-specific protein Bnm1 [Brassica napus] GI:1857671; contains Pfam profile PF04043: Plant invertase/pectin methylesterase inhibitor Length = 166 Score = 27.9 bits (59), Expect = 6.6 Identities = 12/33 (36%), Positives = 18/33 (54%) Frame = +1 Query: 82 DLIRCVCVIITIKMVAGGKQMNVRVTTMDAELE 180 D+ RC + + +K V GG + N T MD L+ Sbjct: 117 DIFRCTDITMYLKDVVGGNRDNTSKTFMDMTLQ 149 >At4g24240.1 68417.m03479 WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA -binding domain Length = 353 Score = 27.9 bits (59), Expect = 6.6 Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 2/39 (5%) Frame = +1 Query: 298 LGSSCIRR*CNKTSRKRIHCSS--NSEPNSILRMSTMSS 408 L S+ ++R CN + R HCS S ++R+ +SS Sbjct: 236 LSSASLKRRCNSSPSSRCHCSKKRKSRVKRVIRVPAVSS 274 >At3g22060.1 68416.m02784 receptor protein kinase-related contains Pfam profile: PF01657 Domain of unknown function that is usually associated with protein kinase domain Pfam:PF00069 Length = 252 Score = 27.5 bits (58), Expect = 8.8 Identities = 15/46 (32%), Positives = 20/46 (43%) Frame = +3 Query: 420 TLKLFYLQVKNAILSDEIYCPPETSVLLASTRSKPDTENTIPLCTG 557 T K Y N + S Y P T +ST + P+ N + LC G Sbjct: 47 TSKSLYESNLNNLFSQLSYKVPSTGFAASSTGNTPNNVNGLALCRG 92 >At1g11590.1 68414.m01330 pectin methylesterase, putative similar to fruit-specific pectin methylesterase GI:1617583 from [Lycopersicon esculentum] Length = 524 Score = 27.5 bits (58), Expect = 8.8 Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 3/40 (7%) Frame = -2 Query: 114 SYDHTHT--ADKITSTL-CIIFLKGGSLKPALEPRLSDLV 4 SY++ HT + +TS + CI + G+ K +EP+L DL+ Sbjct: 124 SYENVHTWLSSVLTSYITCIDEIGEGAYKRRVEPKLEDLI 163 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,246,720 Number of Sequences: 28952 Number of extensions: 324237 Number of successful extensions: 1032 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 1005 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1032 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1447936096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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