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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060314.seq
         (685 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g02800.1 68414.m00237 endo-1,4-beta-glucanase / cellulase (CE...    31   0.94 
At5g20200.1 68418.m02406 nucleoporin-related contains weak simil...    30   1.2  
At5g56560.1 68418.m07058 F-box family protein contains F-box dom...    29   2.2  
At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor...    29   2.2  
At4g30160.1 68417.m04289 villin, putative similar to  villin 2 (...    29   3.8  
At5g50060.1 68418.m06199 invertase/pectin methylesterase inhibit...    28   6.6  
At4g24240.1 68417.m03479 WRKY family transcription factor contai...    28   6.6  
At3g22060.1 68416.m02784 receptor protein kinase-related contain...    27   8.8  
At1g11590.1 68414.m01330 pectin methylesterase, putative similar...    27   8.8  

>At1g02800.1 68414.m00237 endo-1,4-beta-glucanase / cellulase (CEL2)
           identical to endo-1,4-beta glucanase; ATCEL2 GI:3132891
           from [Arabidopsis thaliana]
          Length = 501

 Score = 30.7 bits (66), Expect = 0.94
 Identities = 26/76 (34%), Positives = 36/76 (47%)
 Frame = -1

Query: 676 SISRCSCHQFVMLCSHSSRDILCWSVTL*GSKRSLARNPGPVQSGIVFSVSGLDRVEASN 497
           S SR S H    L +H+ +D L  S+     +RS  + P P Q     S SGL    A N
Sbjct: 27  SSSRPSIHHRHHLDNHNYKDALSKSILFFEGQRS-GKLP-PNQRMTWRSNSGLSDGSALN 84

Query: 496 TEVSGGQYISSDKIAF 449
            ++ GG Y + D + F
Sbjct: 85  VDLVGGYYDAGDNMKF 100


>At5g20200.1 68418.m02406 nucleoporin-related contains weak
           similarity to Nucleoporin NUP1 (Nuclear pore protein
           NUP1) (Swiss-Prot:P20676) [Saccharomyces cerevisiae]
          Length = 762

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 12/29 (41%), Positives = 20/29 (68%)
 Frame = +1

Query: 214 LFDQVVKTIGLREVGSSVFSTPTPRATSL 300
           LFD+  + IG ++  S +++TPTP A S+
Sbjct: 213 LFDKAKEPIGGKDANSEIWATPTPLAKSI 241


>At5g56560.1 68418.m07058 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 607

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 14/50 (28%), Positives = 28/50 (56%)
 Frame = +1

Query: 97  VCVIITIKMVAGGKQMNVRVTTMDAELEFAIQQTTTGKQLFDQVVKTIGL 246
           +C ++   +V G K +NV++  MD     ++   +TG+   + + KT+GL
Sbjct: 175 ICPVLERLVVDGTKGVNVKIPNMDVPNLRSLSFRSTGELRIELLRKTLGL 224


>At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2
           (CPK2) identical to calcium-dependent protein kinase
           isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535;
           contains protein kinase domain, Pfam:PF00069; contains
           EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
           INTERPRO:IPR002048
          Length = 646

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 18/70 (25%), Positives = 36/70 (51%)
 Frame = +3

Query: 324 MQQDVKKENPLQFKFRAKFYPEDVDDELIQEITLKLFYLQVKNAILSDEIYCPPETSVLL 503
           +Q + KKE  L  + + K  PE+   E+  E + +   +Q ++A    +    PET+   
Sbjct: 83  IQPEEKKEKVLAEETKQKVVPEESKQEVPPEESKREVVVQPESAKPETKSESKPETTKPE 142

Query: 504 ASTRSKPDTE 533
            ++ +KP+T+
Sbjct: 143 TTSETKPETK 152


>At4g30160.1 68417.m04289 villin, putative similar to  villin 2
           (VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
           (VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
           profiles PF00626: Gelsolin repeat, PF02209: Villin
           headpiece domain
          Length = 974

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
 Frame = -2

Query: 636 APIPRGTSCVGRS-LFEEVNGHWPGIPAPCRAGLCSP 529
           AP P+ ++   RS LFE++    P IP P +A   +P
Sbjct: 828 APAPKSSAIASRSALFEKIPPQEPSIPKPVKASPKTP 864


>At5g50060.1 68418.m06199 invertase/pectin methylesterase inhibitor
           family protein low similarity to pollen-specific protein
           Bnm1 [Brassica napus] GI:1857671; contains Pfam profile
           PF04043: Plant invertase/pectin methylesterase inhibitor
          Length = 166

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 12/33 (36%), Positives = 18/33 (54%)
 Frame = +1

Query: 82  DLIRCVCVIITIKMVAGGKQMNVRVTTMDAELE 180
           D+ RC  + + +K V GG + N   T MD  L+
Sbjct: 117 DIFRCTDITMYLKDVVGGNRDNTSKTFMDMTLQ 149


>At4g24240.1 68417.m03479 WRKY family transcription factor contains
           Pfam profile: PF03106 WRKY DNA -binding domain
          Length = 353

 Score = 27.9 bits (59), Expect = 6.6
 Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 2/39 (5%)
 Frame = +1

Query: 298 LGSSCIRR*CNKTSRKRIHCSS--NSEPNSILRMSTMSS 408
           L S+ ++R CN +   R HCS    S    ++R+  +SS
Sbjct: 236 LSSASLKRRCNSSPSSRCHCSKKRKSRVKRVIRVPAVSS 274


>At3g22060.1 68416.m02784 receptor protein kinase-related contains
           Pfam profile: PF01657 Domain of unknown function that is
           usually associated with protein kinase domain
           Pfam:PF00069
          Length = 252

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 15/46 (32%), Positives = 20/46 (43%)
 Frame = +3

Query: 420 TLKLFYLQVKNAILSDEIYCPPETSVLLASTRSKPDTENTIPLCTG 557
           T K  Y    N + S   Y  P T    +ST + P+  N + LC G
Sbjct: 47  TSKSLYESNLNNLFSQLSYKVPSTGFAASSTGNTPNNVNGLALCRG 92


>At1g11590.1 68414.m01330 pectin methylesterase, putative similar to
           fruit-specific pectin methylesterase GI:1617583 from
           [Lycopersicon esculentum]
          Length = 524

 Score = 27.5 bits (58), Expect = 8.8
 Identities = 15/40 (37%), Positives = 25/40 (62%), Gaps = 3/40 (7%)
 Frame = -2

Query: 114 SYDHTHT--ADKITSTL-CIIFLKGGSLKPALEPRLSDLV 4
           SY++ HT  +  +TS + CI  +  G+ K  +EP+L DL+
Sbjct: 124 SYENVHTWLSSVLTSYITCIDEIGEGAYKRRVEPKLEDLI 163


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,246,720
Number of Sequences: 28952
Number of extensions: 324237
Number of successful extensions: 1032
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1005
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1032
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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