BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060312.seq (670 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC776.02c |dis2|sds1, bws1|serine/threonine protein phosphatas... 202 3e-53 SPCC31H12.05c |sds21||serine/threonine protein phosphatase Sds21... 192 4e-50 SPAC57A7.08 |pzh1||serine/threonine protein phosphatase Pzh1|Sch... 162 4e-41 SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 118 1e-27 SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa... 107 1e-24 SPBC16H5.07c |ppa2||serine/threonine protein phosphatase Ppa2|Sc... 107 2e-24 SPAC22H10.04 |||protein phosphatase type 2A|Schizosaccharomyces ... 100 2e-22 SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatas... 100 2e-22 SPBC26H8.05c |||serine/threonine protein phosphatase |Schizosacc... 99 7e-22 SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosacch... 81 2e-16 SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual 28 1.4 SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosa... 27 1.8 SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase comple... 27 3.2 SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2... 26 4.3 SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchang... 26 5.6 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 25 9.9 SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi... 25 9.9 SPBC26H8.13c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 9.9 SPAC19B12.08 |||peptidase family C54|Schizosaccharomyces pombe|c... 25 9.9 >SPBC776.02c |dis2|sds1, bws1|serine/threonine protein phosphatase PP1|Schizosaccharomyces pombe|chr 2|||Manual Length = 327 Score = 202 bits (494), Expect = 3e-53 Identities = 94/130 (72%), Positives = 103/130 (79%) Frame = +1 Query: 256 KSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS 435 K+REIF+SQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPE+NYLFLGDYVDRGKQS Sbjct: 40 KAREIFISQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPEANYLFLGDYVDRGKQS 99 Query: 436 LETICLXLAYKIKYPENFFLLRGNPSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNCLP 615 LE ICL LAYKIKYPENFF+LRGN + GF K + + FNCLP Sbjct: 100 LEVICLLLAYKIKYPENFFILRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCLP 159 Query: 616 VAAIVDEKIF 645 +AAI+DEKIF Sbjct: 160 IAAIIDEKIF 169 Score = 40.3 bits (90), Expect = 2e-04 Identities = 16/34 (47%), Positives = 27/34 (79%) Frame = +2 Query: 152 ELNIDNVIKKLLKVRGEKPGMNVQLTXIEIKGLC 253 ++++D++I +LL+VRG +PG VQL+ EI+ LC Sbjct: 5 DVDLDSIIDRLLEVRGSRPGRQVQLSEDEIRFLC 38 >SPCC31H12.05c |sds21||serine/threonine protein phosphatase Sds21|Schizosaccharomyces pombe|chr 3|||Manual Length = 322 Score = 192 bits (468), Expect = 4e-50 Identities = 89/129 (68%), Positives = 100/129 (77%) Frame = +1 Query: 259 SREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSL 438 SR IFLSQP+LLELEAPLKICGDIHGQY DLLRLFEYGG+PP++NYLFLGDYVDRGKQSL Sbjct: 38 SRSIFLSQPMLLELEAPLKICGDIHGQYSDLLRLFEYGGYPPDANYLFLGDYVDRGKQSL 97 Query: 439 ETICLXLAYKIKYPENFFLLRGNPSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNCLPV 618 E ICL AYKIKYPENFFLLRGN + GF K ++ + FNC+PV Sbjct: 98 EVICLLFAYKIKYPENFFLLRGNHEFASINRIYGFYDECKRRYSIKLWKTFTDCFNCMPV 157 Query: 619 AAIVDEKIF 645 AA++DEKIF Sbjct: 158 AAVIDEKIF 166 Score = 36.3 bits (80), Expect = 0.004 Identities = 16/34 (47%), Positives = 23/34 (67%) Frame = +2 Query: 152 ELNIDNVIKKLLKVRGEKPGMNVQLTXIEIKGLC 253 + +ID +I+KL+K R KP VQL+ EI+ LC Sbjct: 2 DYDIDAIIEKLVKARNGKPSKQVQLSDAEIRYLC 35 >SPAC57A7.08 |pzh1||serine/threonine protein phosphatase Pzh1|Schizosaccharomyces pombe|chr 1|||Manual Length = 515 Score = 162 bits (394), Expect = 4e-41 Identities = 79/128 (61%), Positives = 88/128 (68%) Frame = +1 Query: 262 REIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLE 441 REIFLSQP LLEL P+KI GD+HGQY DL+RLFE GFPP SNYLFLGDYVDRGKQSLE Sbjct: 227 REIFLSQPTLLELTPPVKIVGDVHGQYSDLIRLFEMCGFPPSSNYLFLGDYVDRGKQSLE 286 Query: 442 TICLXLAYKIKYPENFFLLRGNPSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNCLPVA 621 TI L YKI+YPENFFLLRGN GF K + + FNCLP+A Sbjct: 287 TILLLFLYKIRYPENFFLLRGNHECANITRVYGFYDECKRRCNIKIWKTFINTFNCLPIA 346 Query: 622 AIVDEKIF 645 ++V KIF Sbjct: 347 SVVAGKIF 354 >SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosaccharomyces pombe|chr 2|||Manual Length = 554 Score = 118 bits (283), Expect = 1e-27 Identities = 56/128 (43%), Positives = 79/128 (61%) Frame = +1 Query: 256 KSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS 435 K+ EI S+ L+E++AP+ +CGDIHGQYYDL++LFE GG P + YLFLGDYVDRG S Sbjct: 96 KATEILKSEDNLIEVDAPVTVCGDIHGQYYDLMKLFEVGGNPANTQYLFLGDYVDRGYFS 155 Query: 436 LETICLXLAYKIKYPENFFLLRGNPSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNCLP 615 +E + A KI YP+ +LLRGN + F + + +FN LP Sbjct: 156 IECLLYLWALKIWYPKTLWLLRGNHECAHLTDYFTFKLECTHKYNIKVYEACLQSFNALP 215 Query: 616 VAAIVDEK 639 +AAI++++ Sbjct: 216 LAAIMNKQ 223 >SPAC823.15 |ppa1||minor serine/threonine protein phosphatase Ppa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 107 bits (258), Expect = 1e-24 Identities = 50/132 (37%), Positives = 81/132 (61%), Gaps = 3/132 (2%) Frame = +1 Query: 259 SREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSL 438 ++E+ + + + P+ +CGDIHGQ++DL+ LF GG P++NYLF+GDYVDRG S+ Sbjct: 35 AKEVLSVESNVQSVRCPVTVCGDIHGQFHDLMELFNIGGPSPDTNYLFMGDYVDRGYHSV 94 Query: 439 ETICLXLAYKIKYPENFFLLRGN---PSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNC 609 ET+ L +A+KI+YP+ +LRGN + GF + G N + F+ Sbjct: 95 ETVSLLIAFKIRYPQRITILRGNHESRQITQVYGFYDECLRKYG--NANVWQYFTDLFDY 152 Query: 610 LPVAAIVDEKIF 645 LP+ A+++++IF Sbjct: 153 LPLTALIEDRIF 164 >SPBC16H5.07c |ppa2||serine/threonine protein phosphatase Ppa2|Schizosaccharomyces pombe|chr 2|||Manual Length = 322 Score = 107 bits (256), Expect = 2e-24 Identities = 50/133 (37%), Positives = 80/133 (60%), Gaps = 3/133 (2%) Frame = +1 Query: 256 KSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS 435 K+RE+ + + + P+ +CGDIHGQ++DL+ LF+ GG P+ NYLF+GDYVDRG S Sbjct: 47 KAREVLCQENNVQPVRNPVTVCGDIHGQFHDLMELFKIGGDVPDMNYLFMGDYVDRGYHS 106 Query: 436 LETICLXLAYKIKYPENFFLLRGN---PSVPASIGFMGFTMSAKGGITLNYGRRSQTAFN 606 +ET+ L +A K++YP +LRGN + GF + G + N + F+ Sbjct: 107 VETVSLLVAMKLRYPNRITILRGNHESRQITQVYGFYDECLRKYG--SANVWKHFTNLFD 164 Query: 607 CLPVAAIVDEKIF 645 P+ A+++++IF Sbjct: 165 YFPLTALIEDRIF 177 >SPAC22H10.04 |||protein phosphatase type 2A|Schizosaccharomyces pombe|chr 1|||Manual Length = 307 Score = 100 bits (240), Expect = 2e-22 Identities = 51/132 (38%), Positives = 77/132 (58%), Gaps = 3/132 (2%) Frame = +1 Query: 259 SREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSL 438 ++E+ + + ++ L P+ + GDIHGQ+ DLL +F GG P +NYLFLGDYVDRG S+ Sbjct: 29 AKEVLMQESNVVRLSTPITVVGDIHGQFDDLLEIFRIGGPCPYTNYLFLGDYVDRGYYSI 88 Query: 439 ETICLXLAYKIKYPENFFLLRGN---PSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNC 609 ETI L + K++YP+ LLRGN + + GF + G N + F+ Sbjct: 89 ETITLLICLKLRYPKRITLLRGNHESRGITQTYGFYSECLRKYG--NANVWKYFTDIFDF 146 Query: 610 LPVAAIVDEKIF 645 L ++A +D+ IF Sbjct: 147 LTLSATIDDTIF 158 >SPCC1739.12 |ppe1|esp1, ppx1|serine/threonine protein phosphatase Ppe1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 305 Score = 100 bits (239), Expect = 2e-22 Identities = 52/127 (40%), Positives = 71/127 (55%), Gaps = 2/127 (1%) Frame = +1 Query: 268 IFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETI 447 I + + + + P+ +CGDIHGQ+YDLL LF GG P +NY+F+GD+VDRG SLET Sbjct: 32 ILMEESNIQPVRTPVTVCGDIHGQFYDLLELFRVGGELPSTNYIFMGDFVDRGYFSLETF 91 Query: 448 CLXLAYKIKYPENFFLLRGNPSVPASIGFMGF--TMSAKGGITLNYGRRSQTAFNCLPVA 621 L + K +YP+ LLRGN GF K G N + F+ L +A Sbjct: 92 TLFMLLKARYPDKITLLRGNHESRQITQVYGFYDECQTKYG-NANVWKYCCQVFDFLTLA 150 Query: 622 AIVDEKI 642 A++D KI Sbjct: 151 AVIDNKI 157 >SPBC26H8.05c |||serine/threonine protein phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 348 Score = 98.7 bits (235), Expect = 7e-22 Identities = 54/131 (41%), Positives = 74/131 (56%), Gaps = 3/131 (2%) Frame = +1 Query: 262 REIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLE 441 R+I + + + + +P+ ICGDIHGQ +DLL LF GG P + YLFLGD+VDRG S+E Sbjct: 32 RDILIEESNIQWISSPVTICGDIHGQLHDLLELFRIGGSCPGTKYLFLGDFVDRGFYSVE 91 Query: 442 TICLXLAYKIKYPENFFLLRGN---PSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNCL 612 T L L K KYP+ L+RGN + GF + G + N R F+ L Sbjct: 92 TFLLLLTLKCKYPKEMTLIRGNHESRQITQVYGFYDECVRKYG--SANVWRYCCEIFDYL 149 Query: 613 PVAAIVDEKIF 645 + A+VD K+F Sbjct: 150 SLGALVDGKVF 160 >SPBC3F6.01c |||serine/threonine protein phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 473 Score = 80.6 bits (190), Expect = 2e-16 Identities = 44/130 (33%), Positives = 65/130 (50%), Gaps = 5/130 (3%) Frame = +1 Query: 262 REIFLSQPILLEL----EAPLKICGDIHGQYYDLLRLFEYGGFP-PESNYLFLGDYVDRG 426 +E+ P L+++ + L ICGD HGQY+DLL +F+ G P P + YLF GD+VDRG Sbjct: 192 KELLEKTPSLIDIPVKGDETLVICGDTHGQYFDLLNIFKLHGPPSPTNKYLFNGDFVDRG 251 Query: 427 KQSLETICLXLAYKIKYPENFFLLRGNPSVPASIGFMGFTMSAKGGITLNYGRRSQTAFN 606 S E AYK+ YP+ F+ RGN GF + F+ Sbjct: 252 SWSTEVAFTLYAYKLLYPDAVFINRGNHETDDMNKVYGFEGECRSKYNERTFNIFSETFS 311 Query: 607 CLPVAAIVDE 636 LP+ +++ + Sbjct: 312 LLPLGSLISD 321 >SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 738 Score = 27.9 bits (59), Expect = 1.4 Identities = 11/28 (39%), Positives = 18/28 (64%) Frame = -3 Query: 329 MSPQIFKGASSSSSIGCDKNISLDFNIA 246 +S Q+FK SS S + CD+ ++D +A Sbjct: 567 VSVQVFKDGSSDSEVRCDEYAAVDVMVA 594 >SPAC19A8.08 |upf2||nonsense-mediated decay protein Upf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1049 Score = 27.5 bits (58), Expect = 1.8 Identities = 15/47 (31%), Positives = 26/47 (55%) Frame = -3 Query: 188 LVIFLLRYRCLTHLCLCLPSDILYVYEIWLNRIGKRREMFKIFADYE 48 ++ +LL Y+C + +P D+LY +E + IG R K++ YE Sbjct: 820 MLTYLLAYQCYFLIQPEMPLDMLYEFE---DVIGYVRPSMKVYMHYE 863 >SPBC12C2.10c |pst1|SPBC21D10.01c|Clr6 histone deacetylase complex subunit Pst1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1522 Score = 26.6 bits (56), Expect = 3.2 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = -2 Query: 477 IFNLVGEXQAYCFQRLLATVHIVSEE 400 ++NL G YCF RL T++ EE Sbjct: 1001 VYNLFGNMSLYCFFRLFHTLYSRLEE 1026 >SPBC1198.05 |||guanylate kinase |Schizosaccharomyces pombe|chr 2|||Manual Length = 202 Score = 26.2 bits (55), Expect = 4.3 Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 6/73 (8%) Frame = +1 Query: 253 LKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRL------FEYGGFPPESNYLFLGDY 414 + ++ +FL+ P + +LE L+ G + L RL EY P + L + D Sbjct: 129 IDAQYVFLAPPSIEQLEVRLRGRGT-ENESAILQRLERARAEIEYSEKPGNFDALIVNDD 187 Query: 415 VDRGKQSLETICL 453 V++ + LE ICL Sbjct: 188 VEKAYKQLEAICL 200 >SPAC19A8.01c |sec73|sec7c, SPAC23H3.01|guanyl-nucleotide exchange factor Sec73 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1082 Score = 25.8 bits (54), Expect = 5.6 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +1 Query: 478 PENFFLLRGNPSVPASIGFMGFTMSAK 558 P+N F L+GNP P+S F +S K Sbjct: 951 PDNNFNLKGNPENPSSTFFTPEILSPK 977 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 25.0 bits (52), Expect = 9.9 Identities = 9/33 (27%), Positives = 17/33 (51%) Frame = +2 Query: 380 LRSPTISSSETMWTVASSRWKQYAWXSPTRLNI 478 +R+ I S+ +W+ A W Y+ P R+ + Sbjct: 2097 VRACFIQGSQQVWSAALWYWGSYSTDKPNRMGV 2129 >SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 25.0 bits (52), Expect = 9.9 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = -3 Query: 158 LTHLCLCLPSDILYVYEIWLNRIGKR 81 L HL L P+DIL ++E + + KR Sbjct: 78 LAHLLLSQPTDILPLFESAVTTVAKR 103 >SPBC26H8.13c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 124 Score = 25.0 bits (52), Expect = 9.9 Identities = 10/19 (52%), Positives = 12/19 (63%) Frame = -1 Query: 352 RGHSIVHVCHRKFSKELQV 296 RG HVCHRK + L+V Sbjct: 55 RGFRTCHVCHRKNNMRLKV 73 >SPAC19B12.08 |||peptidase family C54|Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 25.0 bits (52), Expect = 9.9 Identities = 18/49 (36%), Positives = 27/49 (55%), Gaps = 1/49 (2%) Frame = +1 Query: 235 RN*RAMLKSREIFLSQPILLELEAPLKICGDIHGQYYD-LLRLFEYGGF 378 RN A+ + + +S P+LL + L I I+ YYD LL++FE F Sbjct: 159 RNGNAIYRDQLSKVSFPVLLLIPTRLGI-DSINESYYDQLLQVFEIRSF 206 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,832,112 Number of Sequences: 5004 Number of extensions: 59827 Number of successful extensions: 168 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 149 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 155 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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