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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060312.seq
         (670 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_50882| Best HMM Match : Metallophos (HMM E-Value=8.00001e-41)      210   1e-54
SB_49199| Best HMM Match : Metallophos (HMM E-Value=2.5e-25)           96   2e-20
SB_31501| Best HMM Match : No HMM Matches (HMM E-Value=.)              88   5e-18
SB_52368| Best HMM Match : No HMM Matches (HMM E-Value=.)              88   7e-18
SB_38314| Best HMM Match : Metallophos (HMM E-Value=5.1e-07)           86   3e-17
SB_49265| Best HMM Match : efhand (HMM E-Value=0.68)                   58   5e-09
SB_56433| Best HMM Match : Metallophos (HMM E-Value=1.7e-15)           49   4e-06
SB_1044| Best HMM Match : Metallophos (HMM E-Value=5.8e-16)            38   0.007
SB_30320| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   2.6  
SB_57048| Best HMM Match : S-antigen (HMM E-Value=3.4)                 28   7.9  
SB_42654| Best HMM Match : Tfb4 (HMM E-Value=0.0028)                   28   7.9  

>SB_50882| Best HMM Match : Metallophos (HMM E-Value=8.00001e-41)
          Length = 293

 Score =  210 bits (512), Expect = 1e-54
 Identities = 101/131 (77%), Positives = 105/131 (80%)
 Frame = +1

Query: 253 LKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQ 432
           LKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQ
Sbjct: 40  LKSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQ 99

Query: 433 SLETICLXLAYKIKYPENFFLLRGNPSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNCL 612
           SLETICL LAYKIKYPENFFLLRGN    +     GF    K    +   +     FNCL
Sbjct: 100 SLETICLLLAYKIKYPENFFLLRGNHECASINRIYGFYDECKRRYNIKLWKTFTDCFNCL 159

Query: 613 PVAAIVDEKIF 645
           PVAAI+DEKIF
Sbjct: 160 PVAAILDEKIF 170



 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 21/37 (56%), Positives = 31/37 (83%)
 Frame = +2

Query: 143 DTDELNIDNVIKKLLKVRGEKPGMNVQLTXIEIKGLC 253
           + ++LN+D++I +LL+VRG +PG NVQLT  EI+GLC
Sbjct: 3   EPEKLNVDSIISRLLEVRGSRPGKNVQLTEAEIRGLC 39


>SB_49199| Best HMM Match : Metallophos (HMM E-Value=2.5e-25)
          Length = 173

 Score = 96.3 bits (229), Expect = 2e-20
 Identities = 52/129 (40%), Positives = 72/129 (55%), Gaps = 3/129 (2%)
 Frame = +1

Query: 265 EIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLET 444
           E+ L +  +  + +P+ +CGDIHGQ+YDL  LF  GG  P ++Y+F+GD+VDRG  SLET
Sbjct: 31  ELLLEESNVQPVSSPVTVCGDIHGQFYDLEELFRTGGQVPNTSYVFMGDFVDRGYYSLET 90

Query: 445 ICLXLAYKIKYPENFFLLRGN---PSVPASIGFMGFTMSAKGGITLNYGRRSQTAFNCLP 615
               L  K K+P+   LLRGN     +    GF     S  G    N  R     F+ L 
Sbjct: 91  FTRLLTLKAKWPDRITLLRGNHESRQITQVYGFYDECQSKYG--NANAWRYCCKVFDLLT 148

Query: 616 VAAIVDEKI 642
           VAAI+D ++
Sbjct: 149 VAAIIDGQV 157


>SB_31501| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1150

 Score = 88.2 bits (209), Expect = 5e-18
 Identities = 43/80 (53%), Positives = 54/80 (67%)
 Frame = +1

Query: 268 IFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETI 447
           I   +  +LE++AP+ +CGDIHGQ+YDL++LFE GG P  + YLFLGDYVDRG  S+E  
Sbjct: 69  ILRKEKTMLEVDAPITVCGDIHGQFYDLVKLFEVGGSPATTRYLFLGDYVDRGYFSIE-- 126

Query: 448 CLXLAYKIKYPENFFLLRGN 507
           CL       Y    FLLRGN
Sbjct: 127 CL-------YTNTLFLLRGN 139


>SB_52368| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 128

 Score = 87.8 bits (208), Expect = 7e-18
 Identities = 39/58 (67%), Positives = 47/58 (81%)
 Frame = +1

Query: 259 SREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQ 432
           SREIFL QP+LLE+ AP+ I GDIHGQY DLLR F+  G+PP  +Y+FLGDYVDR K+
Sbjct: 44  SREIFLQQPMLLEIGAPINIFGDIHGQYEDLLRHFDKLGYPPNESYIFLGDYVDRPKR 101


>SB_38314| Best HMM Match : Metallophos (HMM E-Value=5.1e-07)
          Length = 199

 Score = 85.8 bits (203), Expect = 3e-17
 Identities = 34/71 (47%), Positives = 52/71 (73%)
 Frame = +1

Query: 256 KSREIFLSQPILLELEAPLKICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQS 435
           K++EI   +  + E++ P+ +CGD+HGQ++DL+ LF  GG  P++NYLF+GDYVDRG  S
Sbjct: 35  KAKEILSKESNVQEVKCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYS 94

Query: 436 LETICLXLAYK 468
           +ET+ L +  K
Sbjct: 95  VETVTLLVTLK 105


>SB_49265| Best HMM Match : efhand (HMM E-Value=0.68)
          Length = 720

 Score = 58.4 bits (135), Expect = 5e-09
 Identities = 26/64 (40%), Positives = 42/64 (65%), Gaps = 4/64 (6%)
 Frame = +1

Query: 262 REIFLSQPILLELEAPLKICGDIHGQYYDLL----RLFEYGGFPPESNYLFLGDYVDRGK 429
           RE+ + +P +L +++P+ + GD+HG + DL+     L+  G     SN+LFLGDYVDRG+
Sbjct: 657 REVVMEEPRMLRVQSPVYVLGDLHGNFRDLVCFEKLLWRMGPVLTPSNFLFLGDYVDRGE 716

Query: 430 QSLE 441
             +E
Sbjct: 717 NGVE 720


>SB_56433| Best HMM Match : Metallophos (HMM E-Value=1.7e-15)
          Length = 417

 Score = 48.8 bits (111), Expect = 4e-06
 Identities = 34/98 (34%), Positives = 48/98 (48%), Gaps = 7/98 (7%)
 Frame = +1

Query: 373 GFP-PESNYLFLGDYVDRGKQSLETICLXLAYKIKYPENFFLLRGNPSVPASIGFMGFTM 549
           G P PE+ Y+F GD VDRG +S+E   +  A+ I YP   ++ RGN      I  +   +
Sbjct: 2   GLPSPENPYIFNGDLVDRGPRSIEICIILFAFTILYPNGVYINRGNHE--DHIMNLRIIV 59

Query: 550 SAKGGITLNYGRRSQTA------FNCLPVAAIVDEKIF 645
           +  GG       RS+           LP+A IV+ KIF
Sbjct: 60  TKAGGKDQYKDSRSEIIRTFRKYLGWLPLATIVNNKIF 97


>SB_1044| Best HMM Match : Metallophos (HMM E-Value=5.8e-16)
          Length = 250

 Score = 37.9 bits (84), Expect = 0.007
 Identities = 22/64 (34%), Positives = 31/64 (48%)
 Frame = +1

Query: 316 ICGDIHGQYYDLLRLFEYGGFPPESNYLFLGDYVDRGKQSLETICLXLAYKIKYPENFFL 495
           + GDIHG    L +LFE          +FLGDYVD   +S  TI   +  +I    +   
Sbjct: 7   VFGDIHGGLRALQQLFERAEITINDKLIFLGDYVDGWSESAATIQFLI--EIAEKHDCIF 64

Query: 496 LRGN 507
           ++GN
Sbjct: 65  IKGN 68


>SB_30320| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 86

 Score = 29.5 bits (63), Expect = 2.6
 Identities = 18/58 (31%), Positives = 25/58 (43%)
 Frame = -3

Query: 332 CMSPQIFKGASSSSSIGCDKNISLDFNIAL*FLXPSTEHSYQASHLVLLVIFLLRYRC 159
           C S  +  G  S S   C+  ++  +N    FL  S+ H Y  S   L  + LLR  C
Sbjct: 20  CFSVNVASGKDSESKFWCELLVTDKYNAPDKFLPNSSSHHYSIS---LKALMLLRELC 74


>SB_57048| Best HMM Match : S-antigen (HMM E-Value=3.4)
          Length = 242

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 14/32 (43%), Positives = 16/32 (50%)
 Frame = -1

Query: 439 PTTACHGPHSLRGRDSWTPEGSHHTQTTLRGH 344
           PT   HGPH+     +WTP G  HT  T   H
Sbjct: 202 PTRTPHGPHT---DPTWTPHGP-HTDPTRNPH 229


>SB_42654| Best HMM Match : Tfb4 (HMM E-Value=0.0028)
          Length = 128

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 18/61 (29%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
 Frame = -1

Query: 529 LLMLAHSGFRVKERNSRD-I*SCRRGPGILFPTTACHGPHSLRGRDSWTPEGSHHTQTTL 353
           L++L +S   +K  N    + +   G   L+P  +C G  SL  +DS   + S    T L
Sbjct: 42  LMVLCNSYLLLKHNNLLAFVAASTNGSKFLYPKASCEGIQSLPSQDSKYEKFSEFNDTVL 101

Query: 352 R 350
           R
Sbjct: 102 R 102


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,893,237
Number of Sequences: 59808
Number of extensions: 439318
Number of successful extensions: 1155
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1052
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1148
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1729817375
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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