Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060310.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing ... 31 0.71
At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing ... 31 0.71
At1g59453.1 68414.m06679 transcription factor-related weak simil... 31 0.71
At2g46180.1 68415.m05742 intracellular protein transport protein... 30 1.6
At1g78270.1 68414.m09121 UDP-glucose glucosyltransferase, putati... 30 1.6
At4g32700.1 68417.m04655 DNA-directed DNA polymerase family prot... 29 2.9
At4g26050.1 68417.m03750 leucine-rich repeat family protein cont... 29 2.9
At2g35110.1 68415.m04307 HEM protein-related weak similarity to ... 29 2.9
At1g19230.1 68414.m02393 respiratory burst oxidase protein E (Rb... 29 2.9
At4g10270.1 68417.m01688 wound-responsive family protein similar... 29 3.8
At1g53720.1 68414.m06113 cyclophilin-RNA interacting protein, pu... 28 5.0
At3g08680.2 68416.m01009 leucine-rich repeat transmembrane prote... 28 6.6
At3g08680.1 68416.m01008 leucine-rich repeat transmembrane prote... 28 6.6
At2g38720.1 68415.m04755 microtubule associated protein (MAP65/A... 28 6.6
At5g48310.1 68418.m05968 expressed protein 27 8.8
>At5g66030.2 68418.m08315 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 765
Score = 31.1 bits (67), Expect = 0.71
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +1
Query: 163 EQADQLRAEWSRELARVEDEIATLRTVLQSKSV 261
E AD+L E RE++R+ DE+ LR ++SK V
Sbjct: 585 ELADRLIEEKDREISRLVDEMTNLRKSMESKPV 617
>At5g66030.1 68418.m08314 Golgi-localized GRIP domain-containing
protein contains Pfam profile PF01465: GRIP domain;
supporting cDNA gi|20303028|gb|AF499634.1|
Length = 788
Score = 31.1 bits (67), Expect = 0.71
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +1
Query: 163 EQADQLRAEWSRELARVEDEIATLRTVLQSKSV 261
E AD+L E RE++R+ DE+ LR ++SK V
Sbjct: 585 ELADRLIEEKDREISRLVDEMTNLRKSMESKPV 617
>At1g59453.1 68414.m06679 transcription factor-related weak similarity
to TFIIIC Box B-binding subunit [Homo sapiens] GI:442362
Length = 1729
Score = 31.1 bits (67), Expect = 0.71
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = +3
Query: 303 KEITEDVNQGLKNVKESQVYQKTESVIKTTAEKTSSIIGGITAGVSSKLGQMRNSESFR 479
K ++D N+ ++N S +K S++KT E SII ++S NSESF+
Sbjct: 907 KSNSKDKNKAVENSPSSSKKRKRASLVKTKGEGVKSIIVDGQKVLNSDAIDASNSESFQ 965
>At2g46180.1 68415.m05742 intracellular protein transport protein
USO1-related similar to Intracellular protein transport
protein USO1 (Swiss-Prot:P25386) [Saccharomyces
cerevisiae]
Length = 725
Score = 29.9 bits (64), Expect = 1.6
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 169 ADQLRAEWSRELARVEDEIATLRTVLQSKSVK 264
A+ + AEW +++VED+ A +R VL+ +
Sbjct: 528 AENIAAEWKNRVSKVEDDNAKVRRVLEQSMTR 559
>At1g78270.1 68414.m09121 UDP-glucose glucosyltransferase, putative
similar to UDP-glucose glucosyltransferase GI:3928543
from [Arabidopsis thaliana]
Length = 489
Score = 29.9 bits (64), Expect = 1.6
Identities = 16/65 (24%), Positives = 36/65 (55%)
Frame = +3
Query: 231 FENCTAKQIRQSSDLKRKLGITVWKEITEDVNQGLKNVKESQVYQKTESVIKTTAEKTSS 410
F+ ++I ++S++ RKLG+ +W+E TE ++ +++ +Y S+ T+E+
Sbjct: 258 FQILENREIDKNSEI-RKLGLNLWEEETESLDWLDTKAEKAVIYVNFGSLTVLTSEQILE 316
Query: 411 IIGGI 425
G+
Sbjct: 317 FAWGL 321
>At4g32700.1 68417.m04655 DNA-directed DNA polymerase family protein
similar to DNA helicase HEL308 [Homo sapiens]
GI:19110782; contains Pfam profiles PF00271: Helicase
conserved C-terminal domain, PF00176: SNF2 family
N-terminal domain, PF00476: DNA polymerase family A
Length = 1548
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/36 (38%), Positives = 20/36 (55%)
Frame = +3
Query: 396 EKTSSIIGGITAGVSSKLGQMRNSESFRSIEERVGS 503
EK S ++G T GVSS++ +FR I VG+
Sbjct: 802 EKPSEVLGDATLGVSSEINLTSRLPNFRPIGTAVGT 837
>At4g26050.1 68417.m03750 leucine-rich repeat family protein
contains Pfam PF00560: Leucine Rich Repeat domains;
Length = 383
Score = 29.1 bits (62), Expect = 2.9
Identities = 20/53 (37%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +3
Query: 315 EDVNQGLKNVKESQVYQKTESVIKTTAEKTSSIIGGITA-GVSSKLGQMRNSE 470
E V QGL+ +K+ + TES KT +K S IG + G+SS G+ E
Sbjct: 286 EVVEQGLEALKQYMSEKMTESYKKTPTKKKSWGIGKLVKYGLSSSPGRSTGRE 338
>At2g35110.1 68415.m04307 HEM protein-related weak similarity to
Membrane-associated protein Hem (Dhem-2)
(Swiss-Prot:P55162) [Drosophila melanogaster]; weak
similarity to Nck-associated protein 1 (NAP 1)
(p125Nap1) (Membrane-associated protein HEM-2)
(Swiss-Prot:P55161) [Rattus norvegicus]
Length = 1339
Score = 29.1 bits (62), Expect = 2.9
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = -1
Query: 439 DTPAVMPPMIEDVFSAVVFITDSVFWY 359
D P+++ P I+ VFSA+ V WY
Sbjct: 417 DQPSLLAPNIQMVFSALALAQSEVLWY 443
>At1g19230.1 68414.m02393 respiratory burst oxidase protein E
(RbohE) / NADPH oxidase nearly identical to respiratory
burst oxidase protein E GI:3242787 [gi:3242787] from
[Arabidopsis thaliana]
Length = 926
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/25 (56%), Positives = 16/25 (64%)
Frame = +2
Query: 32 HSFHTVRVFHCKI*AVFKQLKKQCQ 106
H TV VF+C I V K+LKKQ Q
Sbjct: 885 HPNSTVGVFYCGIQTVAKELKKQAQ 909
>At4g10270.1 68417.m01688 wound-responsive family protein similar to
wound induced protein (GI:19320) [Lycopersicon
esculentum]
Length = 90
Score = 28.7 bits (61), Expect = 3.8
Identities = 18/46 (39%), Positives = 22/46 (47%)
Frame = +3
Query: 273 LKRKLGITVWKEITEDVNQGLKNVKESQVYQKTESVIKTTAEKTSS 410
LK +LG+ W I VNQ L+N S K S +A TSS
Sbjct: 20 LKDQLGLCRWNYILRSVNQHLRNNVRSVSQGKRFSSSSVSAAVTSS 65
>At1g53720.1 68414.m06113 cyclophilin-RNA interacting protein,
putative
Length = 506
Score = 28.3 bits (60), Expect = 5.0
Identities = 22/70 (31%), Positives = 30/70 (42%), Gaps = 3/70 (4%)
Frame = +2
Query: 299 VERDHRGRQS--GFEKRQRKPSIPKN*ICDKNDRRE-DVFDHRRHHGRRVQQARSDAQLG 469
+ER HR R+ E R+ + DK RRE D DHR H + ++ D + G
Sbjct: 437 LERKHRERKERESREDEDRRRRRRREESRDKESRRERDEDDHRSHRDYKERRRERDDRHG 496
Query: 470 VFPLHRRTRR 499
H R R
Sbjct: 497 REARHERRDR 506
>At3g08680.2 68416.m01009 leucine-rich repeat transmembrane protein
kinase, putative contains Pfam profile: PF00069
Eukaryotic protein kinase domain, PF00560 leucine Rich
Repeat (5 copies)
Length = 640
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = -2
Query: 591 VAPLASRSASSKL*VEFDLEATLPLTFSKPNRRVLRWSGKTPSCAS 454
V SR S+ +E D +A L P+ R L W+ P CAS
Sbjct: 13 VTTFVSRCLSAD--IESDKQALLEFASLVPHSRKLNWNSTIPICAS 56
>At3g08680.1 68416.m01008 leucine-rich repeat transmembrane protein
kinase, putative contains Pfam profile: PF00069
Eukaryotic protein kinase domain, PF00560 leucine Rich
Repeat (5 copies)
Length = 640
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = -2
Query: 591 VAPLASRSASSKL*VEFDLEATLPLTFSKPNRRVLRWSGKTPSCAS 454
V SR S+ +E D +A L P+ R L W+ P CAS
Sbjct: 13 VTTFVSRCLSAD--IESDKQALLEFASLVPHSRKLNWNSTIPICAS 56
>At2g38720.1 68415.m04755 microtubule associated protein
(MAP65/ASE1) family protein low similarity to myosin
[Schistosoma japonicum] GI:3941320; contains Pfam
profile PF03999: Microtubule associated protein
(MAP65/ASE1 family)
Length = 587
Score = 27.9 bits (59), Expect = 6.6
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +1
Query: 163 EQADQLRAEWSRELARVEDEIATLRTVLQSK 255
E+ + RAE R LA+ E EIA+L + L K
Sbjct: 50 EKTRKFRAELQRSLAQAEAEIASLMSALGEK 80
>At5g48310.1 68418.m05968 expressed protein
Length = 1156
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/50 (30%), Positives = 23/50 (46%)
Frame = +1
Query: 124 DIHTPDELAGLTPEQADQLRAEWSRELARVEDEIATLRTVLQSKSVKART 273
DI TP + Q D + E +++ ++EDEI V SK R+
Sbjct: 159 DIRTPSAPPIMESGQEDSISLEIEKDIQKIEDEICGEAGVESSKQESMRS 208
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,094,587
Number of Sequences: 28952
Number of extensions: 237068
Number of successful extensions: 772
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 772
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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