Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060303.seq
(693 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 79 8e-16
SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4 |Schi... 46 4e-06
SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 30 0.36
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 28 1.1
SPBC3D6.04c |mad1||mitotic spindle checkpoint protein Mad1|Schiz... 27 1.9
SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|S... 27 3.4
SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces... 26 5.9
SPCC4B3.17 |cbp3||ubiquinol cytochrome-c reductase assembly prot... 26 5.9
>SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 878
Score = 78.6 bits (185), Expect = 8e-16
Identities = 40/99 (40%), Positives = 65/99 (65%)
Frame = +1
Query: 295 KHQEIVILSMKMEKDVSVRQQAVDLLYAMCDKTNAEEIVQEMLAYLETADYSIREEMVVK 474
KH + +ILS KDVS+R+++++LLY MCD+ NA+ IV ++L YL D +E+++ K
Sbjct: 357 KHYKELILSSLRYKDVSLRKKSLELLYMMCDEENAKLIVADLLQYLPHLDSVTQEDLISK 416
Query: 475 VAILAEKYATDSLGMWDVIPNPIRIAGDYVSEEVXYRVI 591
VAI++E +ATD DV +RIAG + V ++++
Sbjct: 417 VAIISETFATDYEWYVDVTIQLLRIAGKSADDGVWHQLV 455
Score = 50.4 bits (115), Expect = 2e-07
Identities = 31/103 (30%), Positives = 51/103 (49%)
Frame = +2
Query: 74 PPKSKKVQHSNAKNAVLFEAISLIIHNDSEPNLLVRACNQLGQFLSNRETNLRYLALESM 253
P + VQ NA NA+LFEAI L D +L + ++L ++++E+N+RYLA E+
Sbjct: 284 PNDNSNVQQVNAVNAILFEAIKLAFLVDESHSLYEKCMDRLADMIADKESNIRYLAFETT 343
Query: 254 FI*LHQNFLMKQ*KSIKK**SSQ*KWKKMFLSGSRL*IFCMQC 382
+ + K K+ S ++K + L L + M C
Sbjct: 344 AYLISCGHSITSLKHYKELILSSLRYKDVSLRKKSLELLYMMC 386
Score = 25.8 bits (54), Expect = 5.9
Identities = 8/22 (36%), Positives = 15/22 (68%)
Frame = +2
Query: 596 IVINRDEVQAYAAKTVFGSITS 661
+++N +E+Q YA K +F + S
Sbjct: 457 VIVNNEEIQEYATKRLFSLLQS 478
>SPCP1E11.06 |apl4||AP-1 adaptor complex gamma subunit Apl4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 865
Score = 46.4 bits (105), Expect = 4e-06
Identities = 21/50 (42%), Positives = 33/50 (66%)
Frame = +2
Query: 104 NAKNAVLFEAISLIIHNDSEPNLLVRACNQLGQFLSNRETNLRYLALESM 253
NA NA+L++A+ I+ +S+ +L V N L +FL NR+ N RY+AL +
Sbjct: 311 NAGNAILYQAVRTILDLNSDSSLRVLGVNILAKFLGNRDNNTRYVALNML 360
>SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1237
Score = 29.9 bits (64), Expect = 0.36
Identities = 16/44 (36%), Positives = 25/44 (56%)
Frame = -2
Query: 440 AVSK*ANISCTISSALVLSHIAYRRSTACCLTETSFSIFIERIT 309
++SK S T+ + L+L + YR ST L +SFS +E+ T
Sbjct: 20 SISKDELYSWTLKNVLILFLVQYRASTPSALQTSSFSTPVEKKT 63
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1428
Score = 28.3 bits (60), Expect = 1.1
Identities = 16/45 (35%), Positives = 26/45 (57%)
Frame = +1
Query: 229 ALFSS*INVHLATSEFSHEAVKKHQEIVILSMKMEKDVSVRQQAV 363
AL S IN++ + +S +A K H+EI+ L++K + D Q V
Sbjct: 745 ALPKSKINLNNSKGIWSKDASKHHREIISLNVKQKMDPYKHQNKV 789
>SPBC3D6.04c |mad1||mitotic spindle checkpoint protein
Mad1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 27.5 bits (58), Expect = 1.9
Identities = 11/33 (33%), Positives = 23/33 (69%)
Frame = +1
Query: 367 LLYAMCDKTNAEEIVQEMLAYLETADYSIREEM 465
LL+ + +KTN E ++E + LE++ ++R+E+
Sbjct: 481 LLFQVKEKTNIEAFLREQIITLESSIATLRQEL 513
>SPAC3H1.04c |mdm31||mitochondrial inner membrane protein
Mdm31|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 26.6 bits (56), Expect = 3.4
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = -3
Query: 163 FTVVVYNKTYCFKKNSILGIGMLHFFRFWWFLSFV 59
F V+ K++ FK+N L + + F WW +S +
Sbjct: 110 FRVLKKMKSFLFKQNKPLTVDNVTAFFSWWLVSHI 144
>SPBC646.03 |||glutamyl-tRNA amidotransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 471
Score = 25.8 bits (54), Expect = 5.9
Identities = 18/72 (25%), Positives = 30/72 (41%), Gaps = 1/72 (1%)
Frame = +1
Query: 307 IVILSMKMEKDVSVRQQAVDLLYAMCDKTNAEEIVQEMLA-YLETADYSIREEMVVKVAI 483
+ I + +EK + ++ + + + +C KTN ML Y D SI E + I
Sbjct: 43 VEISKLPLEKKLPLKWKLITVKENICTKTNLTTAASNMLKDYNSPFDASIVESLKKAGGI 102
Query: 484 LAEKYATDSLGM 519
+ K D M
Sbjct: 103 ILGKTNMDEFAM 114
>SPCC4B3.17 |cbp3||ubiquinol cytochrome-c reductase assembly protein
Cbp3|Schizosaccharomyces pombe|chr 3|||Manual
Length = 283
Score = 25.8 bits (54), Expect = 5.9
Identities = 13/31 (41%), Positives = 16/31 (51%)
Frame = +2
Query: 77 PKSKKVQHSNAKNAVLFEAISLIIHNDSEPN 169
PK++KV S A FE + L IH D N
Sbjct: 158 PKNEKVAFSQALTTRFFEDMELRIHKDYRIN 188
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,457,200
Number of Sequences: 5004
Number of extensions: 42641
Number of successful extensions: 113
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 321951680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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