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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060299.seq
         (556 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g34150.1 68414.m04236 tRNA pseudouridine synthase family prot...    31   0.39 
At2g42450.1 68415.m05252 lipase class 3 family protein similar t...    29   2.1  
At5g17370.1 68418.m02036 WD-40 repeat family protein contains 1 ...    28   3.6  
At4g15180.1 68417.m02328 SET domain-containing protein contains ...    28   3.6  
At5g51490.1 68418.m06386 pectinesterase family protein contains ...    27   6.4  

>At1g34150.1 68414.m04236 tRNA pseudouridine synthase family protein
           similar to pseudouridine synthase 3 [Mus musculus]
           GI:9652099; contains Pfam profile PF01416: tRNA
           pseudouridine synthase
          Length = 446

 Score = 31.5 bits (68), Expect = 0.39
 Identities = 15/41 (36%), Positives = 20/41 (48%)
 Frame = -2

Query: 216 NRALEDLLSPLAWIPPPVSRVQYRSCMVRMYQRFVAQSNSN 94
           NRAL D +  + W P P+      SC  R Y+ F  + N N
Sbjct: 192 NRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWRQNLN 232


>At2g42450.1 68415.m05252 lipase class 3 family protein similar to
           calmodulin-binding heat-shock protein CaMBP [Nicotiana
           tabacum] GI:1087073; contains Pfam profile PF01764:
           Lipase, PF03893: Lipase 3 N-terminal region
          Length = 546

 Score = 29.1 bits (62), Expect = 2.1
 Identities = 16/51 (31%), Positives = 25/51 (49%)
 Frame = +2

Query: 17  DKYVSVKQGVDVTANQDEISSHETFQLEFDWATKRWYIRTMQDRYWTLETG 169
           +K    + G+D  ++ DE +    ++L+  W TK      MQ R W L TG
Sbjct: 73  EKRRQFESGIDNDSSSDEDTGDGKWKLDLAWLTKA-LEPAMQLRRWALPTG 122


>At5g17370.1 68418.m02036 WD-40 repeat family protein contains 1
           significant, 2 weak WD-40 repeats (PF00400); similar to
           transducin beta-like 1 protein.(SP:O60907) [Homo
           sapiens]
          Length = 467

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 18/64 (28%), Positives = 34/64 (53%), Gaps = 3/64 (4%)
 Frame = +2

Query: 32  VKQGVDVTANQDEISSHETFQLEFDWATKRWYIRTMQD---RYWTLETGGGIQASGDNKS 202
           VK+GV V   +  ++SH   +   D  ++R+ +    D   R W++++G   Q   +NK 
Sbjct: 373 VKRGVGVQTYEGHVNSHTPIEFGID-PSERFILSGGDDCYTRIWSIKSG---QLMSENKF 428

Query: 203 SNAL 214
           SN++
Sbjct: 429 SNSV 432


>At4g15180.1 68417.m02328 SET domain-containing protein contains Pfam
            profile PF00856: SET domain
          Length = 2326

 Score = 28.3 bits (60), Expect = 3.6
 Identities = 12/30 (40%), Positives = 19/30 (63%)
 Frame = +2

Query: 14   NDKYVSVKQGVDVTANQDEISSHETFQLEF 103
            +DKYVS ++G+ V  N++     E F +EF
Sbjct: 1763 DDKYVSYRKGLGVVCNKEGGFGEEDFVVEF 1792


>At5g51490.1 68418.m06386 pectinesterase family protein contains
           Pfam profile: PF01095 pectinesterase
          Length = 536

 Score = 27.5 bits (58), Expect = 6.4
 Identities = 12/35 (34%), Positives = 19/35 (54%)
 Frame = -1

Query: 268 CRCWREGHGAVSLPVQLEQSVRRLVVSAGLDTAAS 164
           C+C+ + H     P QL +  R ++V A +D A S
Sbjct: 47  CKCYFKNHNGFQQPTQLSE-FRVMLVEAAMDRAIS 80


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,280,446
Number of Sequences: 28952
Number of extensions: 219655
Number of successful extensions: 829
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 813
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 829
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1053014392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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