BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060299.seq (556 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g34150.1 68414.m04236 tRNA pseudouridine synthase family prot... 31 0.39 At2g42450.1 68415.m05252 lipase class 3 family protein similar t... 29 2.1 At5g17370.1 68418.m02036 WD-40 repeat family protein contains 1 ... 28 3.6 At4g15180.1 68417.m02328 SET domain-containing protein contains ... 28 3.6 At5g51490.1 68418.m06386 pectinesterase family protein contains ... 27 6.4 >At1g34150.1 68414.m04236 tRNA pseudouridine synthase family protein similar to pseudouridine synthase 3 [Mus musculus] GI:9652099; contains Pfam profile PF01416: tRNA pseudouridine synthase Length = 446 Score = 31.5 bits (68), Expect = 0.39 Identities = 15/41 (36%), Positives = 20/41 (48%) Frame = -2 Query: 216 NRALEDLLSPLAWIPPPVSRVQYRSCMVRMYQRFVAQSNSN 94 NRAL D + + W P P+ SC R Y+ F + N N Sbjct: 192 NRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFWRQNLN 232 >At2g42450.1 68415.m05252 lipase class 3 family protein similar to calmodulin-binding heat-shock protein CaMBP [Nicotiana tabacum] GI:1087073; contains Pfam profile PF01764: Lipase, PF03893: Lipase 3 N-terminal region Length = 546 Score = 29.1 bits (62), Expect = 2.1 Identities = 16/51 (31%), Positives = 25/51 (49%) Frame = +2 Query: 17 DKYVSVKQGVDVTANQDEISSHETFQLEFDWATKRWYIRTMQDRYWTLETG 169 +K + G+D ++ DE + ++L+ W TK MQ R W L TG Sbjct: 73 EKRRQFESGIDNDSSSDEDTGDGKWKLDLAWLTKA-LEPAMQLRRWALPTG 122 >At5g17370.1 68418.m02036 WD-40 repeat family protein contains 1 significant, 2 weak WD-40 repeats (PF00400); similar to transducin beta-like 1 protein.(SP:O60907) [Homo sapiens] Length = 467 Score = 28.3 bits (60), Expect = 3.6 Identities = 18/64 (28%), Positives = 34/64 (53%), Gaps = 3/64 (4%) Frame = +2 Query: 32 VKQGVDVTANQDEISSHETFQLEFDWATKRWYIRTMQD---RYWTLETGGGIQASGDNKS 202 VK+GV V + ++SH + D ++R+ + D R W++++G Q +NK Sbjct: 373 VKRGVGVQTYEGHVNSHTPIEFGID-PSERFILSGGDDCYTRIWSIKSG---QLMSENKF 428 Query: 203 SNAL 214 SN++ Sbjct: 429 SNSV 432 >At4g15180.1 68417.m02328 SET domain-containing protein contains Pfam profile PF00856: SET domain Length = 2326 Score = 28.3 bits (60), Expect = 3.6 Identities = 12/30 (40%), Positives = 19/30 (63%) Frame = +2 Query: 14 NDKYVSVKQGVDVTANQDEISSHETFQLEF 103 +DKYVS ++G+ V N++ E F +EF Sbjct: 1763 DDKYVSYRKGLGVVCNKEGGFGEEDFVVEF 1792 >At5g51490.1 68418.m06386 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 536 Score = 27.5 bits (58), Expect = 6.4 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = -1 Query: 268 CRCWREGHGAVSLPVQLEQSVRRLVVSAGLDTAAS 164 C+C+ + H P QL + R ++V A +D A S Sbjct: 47 CKCYFKNHNGFQQPTQLSE-FRVMLVEAAMDRAIS 80 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,280,446 Number of Sequences: 28952 Number of extensions: 219655 Number of successful extensions: 829 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 813 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 829 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1053014392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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