BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060297.seq (688 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 121 1e-28 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 121 1e-28 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 93 5e-20 SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 60 3e-10 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 46 5e-06 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 46 7e-06 SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 43 4e-05 SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 34 0.022 SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 34 0.022 SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 34 0.022 SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 30 0.27 SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 27 1.9 SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch... 27 2.5 SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac... 27 2.5 SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24 famil... 27 3.4 SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 26 4.4 SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe... 26 5.9 SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ... 25 7.8 SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar... 25 7.8 SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha... 25 7.8 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 121 bits (291), Expect = 1e-28 Identities = 68/144 (47%), Positives = 90/144 (62%) Frame = +1 Query: 256 ETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPXH 435 + RF DTR DEQ+R +TIKSTAIS+F E+ + D+ + P FL+NLIDSP H Sbjct: 53 DARFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEP----ADGTDFLVNLIDSPGH 108 Query: 436 VDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMGPWLFLSS 615 VDFSSEVTAALRVTD QTETVLRQA+ ERI+P++ +NK+ L L Sbjct: 109 VDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRAL-LEL 167 Query: 616 NLKLKNYTRRSSVFXENVNVIIAT 687 + + + + E+VNV+I+T Sbjct: 168 QISQEELYQNFARVVESVNVVIST 191 Score = 79.8 bits (188), Expect = 3e-16 Identities = 37/51 (72%), Positives = 43/51 (84%) Frame = +2 Query: 101 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA 253 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+A Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKA 51 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 121 bits (291), Expect = 1e-28 Identities = 68/144 (47%), Positives = 90/144 (62%) Frame = +1 Query: 256 ETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPXH 435 + RF DTR DEQ+R +TIKSTAIS+F E+ + D+ + P FL+NLIDSP H Sbjct: 53 DARFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEP----ADGTDFLVNLIDSPGH 108 Query: 436 VDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMGPWLFLSS 615 VDFSSEVTAALRVTD QTETVLRQA+ ERI+P++ +NK+ L L Sbjct: 109 VDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRAL-LEL 167 Query: 616 NLKLKNYTRRSSVFXENVNVIIAT 687 + + + + E+VNV+I+T Sbjct: 168 QISQEELYQNFARVVESVNVVIST 191 Score = 79.8 bits (188), Expect = 3e-16 Identities = 37/51 (72%), Positives = 43/51 (84%) Frame = +2 Query: 101 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA 253 MV FT +E+R +M K N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+A Sbjct: 1 MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKA 51 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 92.7 bits (220), Expect = 5e-20 Identities = 64/144 (44%), Positives = 83/144 (57%), Gaps = 2/144 (1%) Frame = +1 Query: 262 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPXHVD 441 RF D R+DE R IT+KS+AIS+FF++ I+ D++ + EK +LINLIDSP HVD Sbjct: 55 RFLDFREDEITRGITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVD 106 Query: 442 FSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMGPWLFLSSNL 621 FSSEV++A R+ D QT TVLRQA +RIK IL +NKM L + L Sbjct: 107 FSSEVSSASRLCDGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDR---LITEL 163 Query: 622 KLKNYTRRSSV--FXENVNVIIAT 687 KL + E VN +I T Sbjct: 164 KLSPIEAHYHLLRLVEQVNAVIGT 187 Score = 48.4 bits (110), Expect = 1e-06 Identities = 20/45 (44%), Positives = 32/45 (71%) Frame = +2 Query: 119 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA 253 +++ + + NIRN +++AHVDHGK+TL DSL++ GII+ A Sbjct: 7 EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLA 51 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 60.1 bits (139), Expect = 3e-10 Identities = 43/121 (35%), Positives = 58/121 (47%), Gaps = 6/121 (4%) Frame = +1 Query: 286 EQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPXHVDFSSEVTAA 465 E+++ ITI+S A +E + N Q+ EK + IN+ID+P H+DF+ EV A Sbjct: 108 EREKGITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERA 165 Query: 466 LRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKM-----GPWLFLSS-NLKL 627 LRV D QT TV RQ + I F+NKM PW + N KL Sbjct: 166 LRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDRMGADPWKVIQQINTKL 225 Query: 628 K 630 K Sbjct: 226 K 226 Score = 35.1 bits (77), Expect = 0.010 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%) Frame = +2 Query: 107 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGII 238 N + E DKKR IRN+ + AH+D GK+T T+ ++ G I Sbjct: 41 NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 46.0 bits (104), Expect = 5e-06 Identities = 31/113 (27%), Positives = 52/113 (46%) Frame = +1 Query: 253 RETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPX 432 R R+TDT E++R ++IKST +++ D + K+ F ID+P Sbjct: 174 RSLRYTDTHYLERERVMSIKSTPLTLAVS------------DMKGKT---FAFQCIDTPG 218 Query: 433 HVDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKM 591 HVDF EV A + ++D T +++ AI + +L +NK+ Sbjct: 219 HVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKV 271 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 45.6 bits (103), Expect = 7e-06 Identities = 23/61 (37%), Positives = 32/61 (52%) Frame = +1 Query: 409 INLIDSPXHVDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNK 588 INLID+P H DF+ EV ++ V D QT+ V +QA I ++F+NK Sbjct: 95 INLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAGVEAQTKVVWKQATKRGIPKVIFVNK 154 Query: 589 M 591 M Sbjct: 155 M 155 Score = 34.7 bits (76), Expect = 0.013 Identities = 13/27 (48%), Positives = 21/27 (77%) Frame = +2 Query: 152 NIRNMSVIAHVDHGKSTLTDSLVSKAG 232 +IRN+ +IAH+D GK+TLT+ ++ G Sbjct: 27 SIRNVGIIAHIDAGKTTLTEKMLYYGG 53 >SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 43.2 bits (97), Expect = 4e-05 Identities = 18/38 (47%), Positives = 28/38 (73%) Frame = +2 Query: 125 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII 238 +RG+ + +RN +VIAH+DHGKSTL+D ++ G+I Sbjct: 50 VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI 85 Score = 35.9 bits (79), Expect = 0.005 Identities = 19/65 (29%), Positives = 32/65 (49%) Frame = +1 Query: 397 KGFLINLIDSPXHVDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPIL 576 + +L+NLID+P HVDF +EV +L + QT + A ++ + I Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQGIQAQTLSNFYMAFSQNLVIIP 181 Query: 577 FMNKM 591 +NK+ Sbjct: 182 VLNKV 186 >SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.022 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 137 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 232 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.022 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 137 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 232 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual Length = 460 Score = 33.9 bits (74), Expect = 0.022 Identities = 16/32 (50%), Positives = 20/32 (62%) Frame = +2 Query: 137 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 232 M K++ N+ VI HVD GKST T L+ K G Sbjct: 1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32 >SPBC9B6.04c |tuf1||mitochondrial translation elongation factor EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 439 Score = 30.3 bits (65), Expect = 0.27 Identities = 13/25 (52%), Positives = 19/25 (76%) Frame = +2 Query: 143 KKRNIRNMSVIAHVDHGKSTLTDSL 217 KK ++ N+ I HVDHGK+TLT ++ Sbjct: 50 KKPHV-NIGTIGHVDHGKTTLTAAI 73 >SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 374 Score = 27.5 bits (58), Expect = 1.9 Identities = 21/76 (27%), Positives = 36/76 (47%), Gaps = 1/76 (1%) Frame = -3 Query: 539 STVSVCTHTPAHXSTTTR-APSVTRSAAVTSEEKSTCXGESIKLIKKPFSLXSRWSGFVM 363 +T S + +P+ STTT +PS + S++ +S S+ S S S S Sbjct: 135 TTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 194 Query: 362 NTKSFSSSSKNIEMAV 315 ++ S SSSS + + + Sbjct: 195 SSSSSSSSSSSSSVPI 210 >SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual Length = 686 Score = 27.1 bits (57), Expect = 2.5 Identities = 9/17 (52%), Positives = 15/17 (88%) Frame = +2 Query: 164 MSVIAHVDHGKSTLTDS 214 ++++ HVDHGK+TL D+ Sbjct: 174 VTLMGHVDHGKTTLLDA 190 >SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 406 Score = 27.1 bits (57), Expect = 2.5 Identities = 21/78 (26%), Positives = 35/78 (44%), Gaps = 1/78 (1%) Frame = +2 Query: 146 KRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAERPVSLTRVRTNKTVASPLNLR-P 322 K+ ++N S + + + AG+ AG ++ SL ++T A+P N R P Sbjct: 155 KKPVKNSSSRDQPVRNSGATSTASLPPAGLRAGRGSQISASLAWLKTGGASAAPSNPRQP 214 Query: 323 SLCSSSLKRKI*YSSQTL 376 S+ RK Y+S L Sbjct: 215 PPTSNFSNRKARYASNGL 232 >SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 195 Score = 26.6 bits (56), Expect = 3.4 Identities = 14/44 (31%), Positives = 22/44 (50%) Frame = -1 Query: 157 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQLPYASNGVRFEKG 26 DIPL + P D + RE + RW + +L + Y ++ F G Sbjct: 102 DIPLRLIQPYDPLSRETVYMRWKELAMLDKTVDYQNHNQSFPFG 145 >SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 662 Score = 26.2 bits (55), Expect = 4.4 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +2 Query: 161 NMSVIAHVDHGKSTLTDSLVSKAGII 238 N+ I HVD GKSTL +++ G++ Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265 >SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe|chr 1|||Manual Length = 697 Score = 25.8 bits (54), Expect = 5.9 Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 2/50 (4%) Frame = -2 Query: 606 EEPRSHFVHEQNRLDAL--GNSLTQYCFGLYTHTSTQXNNHKGSISDTEC 463 EE RS++ + R AL LT+YC G + + NH GS C Sbjct: 337 EEFRSYYEY-MGRPSALDYAKRLTEYCGGAHIWLKREDLNHGGSHKINNC 385 >SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr 3|||Manual Length = 736 Score = 25.4 bits (53), Expect = 7.8 Identities = 13/30 (43%), Positives = 15/30 (50%) Frame = -3 Query: 539 STVSVCTHTPAHXSTTTRAPSVTRSAAVTS 450 S +V T +P H STT P T S A S Sbjct: 297 SFTNVPTSSPLHQSTTANHPEKTPSRAAAS 326 >SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 598 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/32 (34%), Positives = 19/32 (59%) Frame = -1 Query: 157 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 62 D P + +H D ++E H RW + +LLN++ Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348 >SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccharomyces pombe|chr 3|||Manual Length = 1284 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = -3 Query: 152 SASCPSSHGSRLP*NSPF*MVYDFCSIK 69 +ASCP SH L + PF + + C IK Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,534,304 Number of Sequences: 5004 Number of extensions: 47974 Number of successful extensions: 145 Number of sequences better than 10.0: 20 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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