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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060297.seq
         (688 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac...   121   1e-28
SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon...   121   1e-28
SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma...    93   5e-20
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    60   3e-10
SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce...    46   5e-06
SPBC660.10 |||translation elongation factor G|Schizosaccharomyce...    46   7e-06
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po...    43   4e-05
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...    34   0.022
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...    34   0.022
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...    34   0.022
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E...    30   0.27 
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi...    27   1.9  
SPBC1271.15c |||translation initiation factor IF-2Mt|Schizosacch...    27   2.5  
SPCC1393.02c |||non-specific DNA binding protein Spt2 |Schizosac...    27   2.5  
SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24 famil...    27   3.4  
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    26   4.4  
SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces pombe...    26   5.9  
SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr ...    25   7.8  
SPBC19F5.03 |||inositol polyphosphate phosphatase |Schizosacchar...    25   7.8  
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha...    25   7.8  

>SPCP31B10.07 |eft202||translation elongation factor 2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 842

 Score =  121 bits (291), Expect = 1e-28
 Identities = 68/144 (47%), Positives = 90/144 (62%)
 Frame = +1

Query: 256 ETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPXH 435
           + RF DTR DEQ+R +TIKSTAIS+F E+ + D+  +  P         FL+NLIDSP H
Sbjct: 53  DARFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEP----ADGTDFLVNLIDSPGH 108

Query: 436 VDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMGPWLFLSS 615
           VDFSSEVTAALRVTD              QTETVLRQA+ ERI+P++ +NK+   L L  
Sbjct: 109 VDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRAL-LEL 167

Query: 616 NLKLKNYTRRSSVFXENVNVIIAT 687
            +  +   +  +   E+VNV+I+T
Sbjct: 168 QISQEELYQNFARVVESVNVVIST 191



 Score = 79.8 bits (188), Expect = 3e-16
 Identities = 37/51 (72%), Positives = 43/51 (84%)
 Frame = +2

Query: 101 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA 253
           MV FT +E+R +M K  N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+A
Sbjct: 1   MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKA 51


>SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation
           elongation factor 2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 842

 Score =  121 bits (291), Expect = 1e-28
 Identities = 68/144 (47%), Positives = 90/144 (62%)
 Frame = +1

Query: 256 ETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPXH 435
           + RF DTR DEQ+R +TIKSTAIS+F E+ + D+  +  P         FL+NLIDSP H
Sbjct: 53  DARFMDTRADEQERGVTIKSTAISLFAEMTDDDMKDMKEP----ADGTDFLVNLIDSPGH 108

Query: 436 VDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMGPWLFLSS 615
           VDFSSEVTAALRVTD              QTETVLRQA+ ERI+P++ +NK+   L L  
Sbjct: 109 VDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERIRPVVVVNKVDRAL-LEL 167

Query: 616 NLKLKNYTRRSSVFXENVNVIIAT 687
            +  +   +  +   E+VNV+I+T
Sbjct: 168 QISQEELYQNFARVVESVNVVIST 191



 Score = 79.8 bits (188), Expect = 3e-16
 Identities = 37/51 (72%), Positives = 43/51 (84%)
 Frame = +2

Query: 101 MVNFTVDEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA 253
           MV FT +E+R +M K  N+RNMSVIAHVDHGKSTLTDSLV KAGII+ A+A
Sbjct: 1   MVAFTPEEVRNLMGKPSNVRNMSVIAHVDHGKSTLTDSLVQKAGIISAAKA 51


>SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1000

 Score = 92.7 bits (220), Expect = 5e-20
 Identities = 64/144 (44%), Positives = 83/144 (57%), Gaps = 2/144 (1%)
 Frame = +1

Query: 262 RFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPXHVD 441
           RF D R+DE  R IT+KS+AIS+FF++       I+  D++ + EK +LINLIDSP HVD
Sbjct: 55  RFLDFREDEITRGITMKSSAISLFFKV-------ISQNDEK-RVEKDYLINLIDSPGHVD 106

Query: 442 FSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKMGPWLFLSSNL 621
           FSSEV++A R+ D              QT TVLRQA  +RIK IL +NKM     L + L
Sbjct: 107 FSSEVSSASRLCDGAFVLVDAVEGVCSQTITVLRQAWIDRIKVILVINKMDR---LITEL 163

Query: 622 KLKNYTRRSSV--FXENVNVIIAT 687
           KL        +    E VN +I T
Sbjct: 164 KLSPIEAHYHLLRLVEQVNAVIGT 187



 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 20/45 (44%), Positives = 32/45 (71%)
 Frame = +2

Query: 119 DEIRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARA 253
           +++  +   + NIRN +++AHVDHGK+TL DSL++  GII+   A
Sbjct: 7   EKLVSLQKNQENIRNFTLLAHVDHGKTTLADSLLASNGIISSKLA 51


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 60.1 bits (139), Expect = 3e-10
 Identities = 43/121 (35%), Positives = 58/121 (47%), Gaps = 6/121 (4%)
 Frame = +1

Query: 286 EQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPXHVDFSSEVTAA 465
           E+++ ITI+S A    +E     +    N  Q+   EK + IN+ID+P H+DF+ EV  A
Sbjct: 108 EREKGITIQSAATHCTWERTVDQIE--ANEKQKTDFEKSYNINIIDTPGHIDFTIEVERA 165

Query: 466 LRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKM-----GPWLFLSS-NLKL 627
           LRV D              QT TV RQ     +  I F+NKM      PW  +   N KL
Sbjct: 166 LRVLDGAVLVLCAVSGVQSQTITVDRQMRRYNVPRISFVNKMDRMGADPWKVIQQINTKL 225

Query: 628 K 630
           K
Sbjct: 226 K 226



 Score = 35.1 bits (77), Expect = 0.010
 Identities = 18/46 (39%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
 Frame = +2

Query: 107 NFTVDEIRGMMDKKR--NIRNMSVIAHVDHGKSTLTDSLVSKAGII 238
           N  + E     DKKR   IRN+ + AH+D GK+T T+ ++   G I
Sbjct: 41  NLNIQEQLNDNDKKRLKQIRNIGISAHIDSGKTTFTERVLYYTGRI 86


>SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 983

 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 31/113 (27%), Positives = 52/113 (46%)
 Frame = +1

Query: 253 RETRFTDTRKDEQDRCITIKSTAISMFFELEEKDLVFITNPDQRXKSEKGFLINLIDSPX 432
           R  R+TDT   E++R ++IKST +++               D + K+   F    ID+P 
Sbjct: 174 RSLRYTDTHYLERERVMSIKSTPLTLAVS------------DMKGKT---FAFQCIDTPG 218

Query: 433 HVDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNKM 591
           HVDF  EV A + ++D               T  +++ AI   +  +L +NK+
Sbjct: 219 HVDFVDEVAAPMAISDGVVLVVDVIEGVMINTTRIIKHAILHDMPIVLVLNKV 271


>SPBC660.10 |||translation elongation factor G|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 813

 Score = 45.6 bits (103), Expect = 7e-06
 Identities = 23/61 (37%), Positives = 32/61 (52%)
 Frame = +1

Query: 409 INLIDSPXHVDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPILFMNK 588
           INLID+P H DF+ EV  ++ V D              QT+ V +QA    I  ++F+NK
Sbjct: 95  INLIDTPGHADFTFEVERSVAVLDGAVAIIDGSAGVEAQTKVVWKQATKRGIPKVIFVNK 154

Query: 589 M 591
           M
Sbjct: 155 M 155



 Score = 34.7 bits (76), Expect = 0.013
 Identities = 13/27 (48%), Positives = 21/27 (77%)
 Frame = +2

Query: 152 NIRNMSVIAHVDHGKSTLTDSLVSKAG 232
           +IRN+ +IAH+D GK+TLT+ ++   G
Sbjct: 27  SIRNVGIIAHIDAGKTTLTEKMLYYGG 53


>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 646

 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 18/38 (47%), Positives = 28/38 (73%)
 Frame = +2

Query: 125 IRGMMDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAGII 238
           +RG+   +  +RN +VIAH+DHGKSTL+D ++   G+I
Sbjct: 50  VRGIPQNR--VRNWAVIAHIDHGKSTLSDCILKLTGVI 85



 Score = 35.9 bits (79), Expect = 0.005
 Identities = 19/65 (29%), Positives = 32/65 (49%)
 Frame = +1

Query: 397 KGFLINLIDSPXHVDFSSEVTAALRVTDXXXXXXXXXXXXXXQTETVLRQAIAERIKPIL 576
           + +L+NLID+P HVDF +EV  +L   +              QT +    A ++ +  I 
Sbjct: 122 QSYLLNLIDTPGHVDFRAEVMHSLAACEGCILLVDASQGIQAQTLSNFYMAFSQNLVIIP 181

Query: 577 FMNKM 591
            +NK+
Sbjct: 182 VLNKV 186


>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score = 33.9 bits (74), Expect = 0.022
 Identities = 16/32 (50%), Positives = 20/32 (62%)
 Frame = +2

Query: 137 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 232
           M K++   N+ VI HVD GKST T  L+ K G
Sbjct: 1   MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score = 33.9 bits (74), Expect = 0.022
 Identities = 16/32 (50%), Positives = 20/32 (62%)
 Frame = +2

Query: 137 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 232
           M K++   N+ VI HVD GKST T  L+ K G
Sbjct: 1   MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score = 33.9 bits (74), Expect = 0.022
 Identities = 16/32 (50%), Positives = 20/32 (62%)
 Frame = +2

Query: 137 MDKKRNIRNMSVIAHVDHGKSTLTDSLVSKAG 232
           M K++   N+ VI HVD GKST T  L+ K G
Sbjct: 1   MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCG 32


>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
           EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 439

 Score = 30.3 bits (65), Expect = 0.27
 Identities = 13/25 (52%), Positives = 19/25 (76%)
 Frame = +2

Query: 143 KKRNIRNMSVIAHVDHGKSTLTDSL 217
           KK ++ N+  I HVDHGK+TLT ++
Sbjct: 50  KKPHV-NIGTIGHVDHGKTTLTAAI 73


>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 374

 Score = 27.5 bits (58), Expect = 1.9
 Identities = 21/76 (27%), Positives = 36/76 (47%), Gaps = 1/76 (1%)
 Frame = -3

Query: 539 STVSVCTHTPAHXSTTTR-APSVTRSAAVTSEEKSTCXGESIKLIKKPFSLXSRWSGFVM 363
           +T S  + +P+  STTT  +PS + S++ +S   S+    S        S  S  S    
Sbjct: 135 TTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 194

Query: 362 NTKSFSSSSKNIEMAV 315
           ++ S SSSS +  + +
Sbjct: 195 SSSSSSSSSSSSSVPI 210


>SPBC1271.15c |||translation initiation factor
           IF-2Mt|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 686

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 9/17 (52%), Positives = 15/17 (88%)
 Frame = +2

Query: 164 MSVIAHVDHGKSTLTDS 214
           ++++ HVDHGK+TL D+
Sbjct: 174 VTLMGHVDHGKTTLLDA 190


>SPCC1393.02c |||non-specific DNA binding protein Spt2
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 406

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 21/78 (26%), Positives = 35/78 (44%), Gaps = 1/78 (1%)
 Frame = +2

Query: 146 KRNIRNMSVIAHVDHGKSTLTDSLVSKAGIIAGARAERPVSLTRVRTNKTVASPLNLR-P 322
           K+ ++N S            + + +  AG+ AG  ++   SL  ++T    A+P N R P
Sbjct: 155 KKPVKNSSSRDQPVRNSGATSTASLPPAGLRAGRGSQISASLAWLKTGGASAAPSNPRQP 214

Query: 323 SLCSSSLKRKI*YSSQTL 376
              S+   RK  Y+S  L
Sbjct: 215 PPTSNFSNRKARYASNGL 232


>SPAC3H1.14 ||SPAC9G1.01|cytoplasmic vesicle protein, Vid24
           family|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 195

 Score = 26.6 bits (56), Expect = 3.4
 Identities = 14/44 (31%), Positives = 22/44 (50%)
 Frame = -1

Query: 157 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQLPYASNGVRFEKG 26
           DIPL +  P D + RE  + RW  + +L   + Y ++   F  G
Sbjct: 102 DIPLRLIQPYDPLSRETVYMRWKELAMLDKTVDYQNHNQSFPFG 145


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 11/26 (42%), Positives = 17/26 (65%)
 Frame = +2

Query: 161 NMSVIAHVDHGKSTLTDSLVSKAGII 238
           N+  I HVD GKSTL  +++   G++
Sbjct: 240 NIVFIGHVDAGKSTLGGNILFLTGMV 265


>SPAC19A8.15 |trp2||tryptophan synthase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 697

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 2/50 (4%)
 Frame = -2

Query: 606 EEPRSHFVHEQNRLDAL--GNSLTQYCFGLYTHTSTQXNNHKGSISDTEC 463
           EE RS++ +   R  AL     LT+YC G +     +  NH GS     C
Sbjct: 337 EEFRSYYEY-MGRPSALDYAKRLTEYCGGAHIWLKREDLNHGGSHKINNC 385


>SPCC285.13c |||nucleoporin Nup60 |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 736

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 13/30 (43%), Positives = 15/30 (50%)
 Frame = -3

Query: 539 STVSVCTHTPAHXSTTTRAPSVTRSAAVTS 450
           S  +V T +P H STT   P  T S A  S
Sbjct: 297 SFTNVPTSSPLHQSTTANHPEKTPSRAAAS 326


>SPBC19F5.03 |||inositol polyphosphate phosphatase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 598

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 11/32 (34%), Positives = 19/32 (59%)
 Frame = -1

Query: 157 DIPLLVHHPTDLVYREIHHFRWFMIFVLLNQL 62
           D P + +H  D  ++E  H RW  + +LLN++
Sbjct: 318 DNPHIHYHYFDF-HKECSHMRWDRVSLLLNEI 348


>SPCC1235.05c |fft2||fun thirty related protein
           Fft2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1284

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 12/28 (42%), Positives = 16/28 (57%)
 Frame = -3

Query: 152 SASCPSSHGSRLP*NSPF*MVYDFCSIK 69
           +ASCP SH   L  + PF  + + C IK
Sbjct: 417 TASCPLSHSKLLLEHRPFQTLAEACIIK 444


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,534,304
Number of Sequences: 5004
Number of extensions: 47974
Number of successful extensions: 145
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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