BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060291.seq
(694 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF388659-4|AAK71996.1| 1308|Apis mellifera NFRKB-like protein pr... 25 0.52
Z26318-1|CAA81227.1| 544|Apis mellifera royal jelly protein RJP... 24 1.2
AF084556-1|AAC71015.1| 652|Apis mellifera pipsqueak protein. 23 2.8
>AF388659-4|AAK71996.1| 1308|Apis mellifera NFRKB-like protein
protein.
Length = 1308
Score = 25.4 bits (53), Expect = 0.52
Identities = 9/17 (52%), Positives = 15/17 (88%)
Frame = -2
Query: 387 SPAAMTLSIDSGALNTM 337
SPA ++S+DSG++NT+
Sbjct: 555 SPAIESISVDSGSINTV 571
Score = 21.4 bits (43), Expect = 8.4
Identities = 8/26 (30%), Positives = 16/26 (61%)
Frame = +3
Query: 105 ITTDNQLNGNAENGGGDSQDHNSAEA 182
+T+ + +N N+ NG +S +S+ A
Sbjct: 532 LTSSSNVNNNSGNGNTNSSARDSSPA 557
>Z26318-1|CAA81227.1| 544|Apis mellifera royal jelly protein
RJP57-1 protein.
Length = 544
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +3
Query: 99 QDITTDNQLNGNAENGGGDSQDHNSAEAPGRDDDRK 206
Q+ N N NA N D+Q+ N ++D+R+
Sbjct: 430 QNADNQNADNQNANNQNADNQNANKQNGNRQNDNRQ 465
Score = 23.8 bits (49), Expect = 1.6
Identities = 10/31 (32%), Positives = 19/31 (61%)
Frame = +3
Query: 114 DNQLNGNAENGGGDSQDHNSAEAPGRDDDRK 206
DN+ NGN +N G+ Q+ N ++D+++
Sbjct: 487 DNKQNGNRQN--GNKQNDNKQNGNRQNDNKR 515
Score = 23.0 bits (47), Expect = 2.8
Identities = 8/20 (40%), Positives = 14/20 (70%)
Frame = +3
Query: 114 DNQLNGNAENGGGDSQDHNS 173
DN+ NGN +N ++Q+ N+
Sbjct: 512 DNKRNGNRQNDNQNNQNDNN 531
Score = 22.2 bits (45), Expect = 4.8
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +3
Query: 117 NQLNGNAENGGGDSQDHNSAEAPGRDDDRK 206
N N NA+N D+Q+ N+ A ++ +++
Sbjct: 426 NAGNQNADNQNADNQNANNQNADNQNANKQ 455
Score = 21.4 bits (43), Expect = 8.4
Identities = 8/29 (27%), Positives = 15/29 (51%)
Frame = +3
Query: 117 NQLNGNAENGGGDSQDHNSAEAPGRDDDR 203
N+ N N NG + + N+ R+D++
Sbjct: 508 NRQNDNKRNGNRQNDNQNNQNDNNRNDNQ 536
Score = 21.4 bits (43), Expect = 8.4
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +3
Query: 114 DNQLNGNAENGGGDSQDHNSAE 179
DNQ N N +N D+Q H+S++
Sbjct: 522 DNQNNQN-DNNRNDNQVHHSSK 542
>AF084556-1|AAC71015.1| 652|Apis mellifera pipsqueak protein.
Length = 652
Score = 23.0 bits (47), Expect = 2.8
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +3
Query: 81 NNDNFAQDITTDNQLNGNAENGGGDSQDHNSAE 179
NN+N + +N N N NG G S ++N+ +
Sbjct: 240 NNNNNNNNNNNNNGANDNG-NGNGASNNNNNGD 271
Score = 22.6 bits (46), Expect = 3.6
Identities = 11/40 (27%), Positives = 17/40 (42%)
Frame = +3
Query: 81 NNDNFAQDITTDNQLNGNAENGGGDSQDHNSAEAPGRDDD 200
N + A + +N N N NG D+ + N A + D
Sbjct: 232 NANTNASNNNNNNNNNNNNNNGANDNGNGNGASNNNNNGD 271
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 195,995
Number of Sequences: 438
Number of extensions: 4011
Number of successful extensions: 10
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 10
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21195810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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