Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060288.seq
(687 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 104 6e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 101 6e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 100 8e-22
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.9
At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 3.8
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.8
At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,... 29 3.8
At5g13880.1 68418.m01622 expressed protein 28 6.7
At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 6.7
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 6.7
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 8.8
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 8.8
At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 8.8
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 104 bits (249), Expect = 6e-23
Identities = 43/72 (59%), Positives = 56/72 (77%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
Score = 104 bits (249), Expect = 6e-23
Identities = 47/86 (54%), Positives = 65/86 (75%), Gaps = 1/86 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLR 436
KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q++
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIK 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIASK 514
+ FD GK+L+ +V+ + GEE + A K
Sbjct: 128 SGFDDGKDLVVSVMSAMGEEQINALK 153
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 101 bits (241), Expect = 6e-22
Identities = 46/86 (53%), Positives = 64/86 (74%), Gaps = 1/86 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 436
KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L TQL+
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLK 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIASK 514
F+ GK+++ +V+ + GEE + A K
Sbjct: 128 NGFEEGKDIVVSVMSAMGEEQMCALK 153
Score = 100 bits (240), Expect = 8e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 100 bits (240), Expect = 8e-22
Identities = 45/86 (52%), Positives = 63/86 (73%), Gaps = 1/86 (1%)
Frame = +2
Query: 260 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 436
KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R
Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMR 127
Query: 437 TDFDSGKELLCTVLKSCGEECVIASK 514
FD GK+++ +V+ S GEE + A K
Sbjct: 128 LGFDEGKDIVVSVMSSMGEEQICAVK 153
Score = 96.3 bits (229), Expect = 2e-20
Identities = 40/72 (55%), Positives = 52/72 (72%)
Frame = +3
Query: 69 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 248
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 249 IFMVKSMKISVP 284
IF K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +2
Query: 398 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 499
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g41460.1 68418.m05035 fringe-related protein strong similarity
to unknown protein (pir||T13026) similarity to predicted
proteins + similar to hypothetical protein GB:AAC23643
[Arabidopsis thaliana] + weak similarity to Fringe
[Schistocerca gregaria](GI:6573138);Fringe encodes an
extracellular protein that regulates Notch signalling.
Length = 524
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = +1
Query: 34 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 120
++ F T Q K++ S P +P P PP P
Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -3
Query: 169 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 62
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At4g25130.1 68417.m03616 peptide methionine sulfoxide reductase,
putative strong similarity to SP|P54151 Peptide
methionine sulfoxide reductase (EC 1.8.4.6) {Brassica
napus}; contains Pfam profile PF01625: Peptide
methionine sulfoxide reductase
Length = 258
Score = 28.7 bits (61), Expect = 3.8
Identities = 21/65 (32%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
Frame = +2
Query: 251 LHGKKYEDICPST--HNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLG 424
+H YED+C T HN V R Y + S + L + + D L GD+G
Sbjct: 126 VHNPSYEDVCTGTTGHN----EVVRVQYDPKECSFESLLDVFWNRHD-PTTLNRQGGDVG 180
Query: 425 TQLRT 439
TQ R+
Sbjct: 181 TQYRS 185
>At5g13880.1 68418.m01622 expressed protein
Length = 188
Score = 27.9 bits (59), Expect = 6.7
Identities = 9/33 (27%), Positives = 17/33 (51%)
Frame = +1
Query: 7 EVGHISFLTVVKFKTQQWVTSKTHTSRPETPGP 105
E+ ++ L + +F Q W+ + P+TP P
Sbjct: 153 EIDKVADLFITRFHRQMWLQKQLSLESPDTPSP 185
>At3g53330.1 68416.m05884 plastocyanin-like domain-containing
protein similar to mavicyanin SP:P80728 from [Cucurbita
pepo]
Length = 310
Score = 27.9 bits (59), Expect = 6.7
Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%)
Frame = +1
Query: 67 SKTHT-SRPETPGPQPPS 117
SKTH SRP TP P PPS
Sbjct: 127 SKTHERSRPITPSPPPPS 144
>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
contains INTERPRO domain, IPR001878: Zn-finger CCHC type
Length = 353
Score = 27.9 bits (59), Expect = 6.7
Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%)
Frame = +1
Query: 70 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 195
KT T P PP PCN PC S + + R CP
Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +2
Query: 311 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 460
V+R+ +++ Y L ADN + + G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
Frame = +2
Query: 341 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 475
+SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At1g20860.1 68414.m02613 phosphate transporter family protein
similar to phosphate transporter [Catharanthus roseus]
GI:2208908, inorganic phosphate transporter 1 [Solanum
tuberosum] GI:1420871; contains Pfam profile PF00083:
major facilitator superfamily protein
Length = 534
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/45 (37%), Positives = 20/45 (44%)
Frame = +1
Query: 46 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 180
K Q V S++H S T P PP P P K S C +H R
Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,225,576
Number of Sequences: 28952
Number of extensions: 314679
Number of successful extensions: 996
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 914
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 982
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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