BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060282.seq (678 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC607.05 |rpn9||19S proteasome regulatory subunit Rpn9|Schizos... 47 2e-06 SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 29 0.47 SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 29 0.47 SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe... 29 0.62 SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosa... 29 0.82 SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 28 1.4 SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Sc... 27 1.9 SPBC3D6.07 |gpi3||pig-A|Schizosaccharomyces pombe|chr 2|||Manual 27 3.3 SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces ... 25 7.6 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 25 7.6 >SPAC607.05 |rpn9||19S proteasome regulatory subunit Rpn9|Schizosaccharomyces pombe|chr 1|||Manual Length = 381 Score = 47.2 bits (107), Expect = 2e-06 Identities = 30/91 (32%), Positives = 46/91 (50%), Gaps = 1/91 (1%) Frame = +1 Query: 31 IDVSDFLTKKQASEP-ALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLY 207 +++SDFL + P +L + +E+LY +KLW QLT L F P + + LY Sbjct: 7 VNMSDFLHDQATRAPESLQQSYILMEDLYERKLWKQLTDALIVFFDTP--ETVPLRLPLY 64 Query: 208 NNFLTTFESKINPLSLLRSLLISWNSMSTRE 300 NF+ +F IN L + L ++ S S E Sbjct: 65 TNFVNSFRPNINQLKAVYMGLKAFESCSNDE 95 Score = 26.6 bits (56), Expect = 3.3 Identities = 17/65 (26%), Positives = 30/65 (46%), Gaps = 1/65 (1%) Frame = +2 Query: 479 VHGRFYKLASNITESVVPLP-YYRAALRYVGCADGGEAYLPLNVLISXSD*LSLECWLPD 655 +H +Y ++++ ++ YYR L Y+ C D + L+ + LS+ L D Sbjct: 157 IHSSYYSVSADYYKAKADYAQYYRHCLLYLSCIDLDKC--SHTELVERAVDLSVAAILGD 214 Query: 656 XYEFG 670 Y FG Sbjct: 215 IYNFG 219 >SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 995 Score = 29.5 bits (63), Expect = 0.47 Identities = 21/60 (35%), Positives = 30/60 (50%) Frame = +1 Query: 88 DWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLYNNFLTTFESKINPLSLLRSL 267 DW + +Y + +TL N A+ RG N+I + LTT E+K NP LL +L Sbjct: 829 DWYDHKVIYRQHN-ENITLSAPLTHINVAI-RGGNIIPMQKPSLTTHETKQNPYDLLVAL 886 >SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 993 Score = 29.5 bits (63), Expect = 0.47 Identities = 14/26 (53%), Positives = 17/26 (65%) Frame = +1 Query: 181 RGDNLIQLYNNFLTTFESKINPLSLL 258 RG N+I L TT+ES+ NP SLL Sbjct: 861 RGGNIIPLQQPGYTTYESRNNPYSLL 886 >SPBC17G9.04c |nup85||nucleoporin Nup85|Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 29.1 bits (62), Expect = 0.62 Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 3/50 (6%) Frame = +1 Query: 100 LEELYNKKLWHQLTLKLQEFVKNPALQRGDNLI---QLYNNFLTTFESKI 240 +E Y WH+L +LQ+ P L NL+ Q Y + F SKI Sbjct: 81 IEPNYLLTAWHELWEELQDTYMTPILDSEANLLLLTQFYGKISSLFRSKI 130 >SPCC550.03c |||RNA helicase involved in mRNA catabolism|Schizosaccharomyces pombe|chr 3|||Manual Length = 1213 Score = 28.7 bits (61), Expect = 0.82 Identities = 27/77 (35%), Positives = 38/77 (49%), Gaps = 4/77 (5%) Frame = +3 Query: 153 RIRQESGPAEGRQPHTVVQ*LFDHLRKQNKSPIIVEIIA-HIVEQYVN---KRDAVTFLE 320 R R + E R +T V L HL KQN P+IV + + E+YV+ RD E Sbjct: 522 RGRGSAKSVERRDANTWVH-LIGHLHKQNLLPVIVFVFSKKRCEEYVDTLTNRDLNNHQE 580 Query: 321 KVETKVKMNDEALALCK 371 K E V + ++A+A K Sbjct: 581 KSEVHVVI-EKAVARLK 596 >SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 27.9 bits (59), Expect = 1.4 Identities = 12/29 (41%), Positives = 20/29 (68%) Frame = +1 Query: 181 RGDNLIQLYNNFLTTFESKINPLSLLRSL 267 RG +++ + LTT+ES+ NP +LL +L Sbjct: 836 RGGSVLPMQQPSLTTYESRQNPFNLLVAL 864 >SPAC1834.02 |aro1||pentafunctional aromatic polypeptide Aro1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1573 Score = 27.5 bits (58), Expect = 1.9 Identities = 11/44 (25%), Positives = 21/44 (47%) Frame = -3 Query: 640 LQRELV*XRYQHVQWQVGLTPVGAPNVPKRCAVVRQWDHGLCNI 509 L R++ + W V +TP G+ ++ R V+ +G C + Sbjct: 391 LSRDVKVDEFTKSSWDVVVTPPGSKSISNRALVLAAMGNGTCRL 434 >SPBC3D6.07 |gpi3||pig-A|Schizosaccharomyces pombe|chr 2|||Manual Length = 456 Score = 26.6 bits (56), Expect = 3.3 Identities = 10/20 (50%), Positives = 15/20 (75%) Frame = -1 Query: 468 PSASSSDFLNVQLFSRLHHN 409 PS +S DFL + + SRL++N Sbjct: 183 PSKASKDFLTIVVISRLYYN 202 >SPBC337.13c |gtr1||Gtr1/RagA G protein Gtr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 308 Score = 25.4 bits (53), Expect = 7.6 Identities = 15/54 (27%), Positives = 27/54 (50%), Gaps = 2/54 (3%) Frame = +3 Query: 255 VEIIAHI--VEQYVNKRDAVTFLEKVETKVKMNDEALALCKVLQGQIYIEQLND 410 V+++ ++ VE +RD VTF +E V + +A C + + + E L D Sbjct: 80 VQVLIYVFDVESREFERDLVTFRNCLEATVANSPQARVFCLIHKMDLVQEDLRD 133 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 25.4 bits (53), Expect = 7.6 Identities = 16/38 (42%), Positives = 26/38 (68%) Frame = -1 Query: 216 KVIVQLYEVVSPLQGRILDEFLQF*SKLVP*FFIVKFL 103 +V+ ++Y VVS +GR++DE ++ + P FFIVK L Sbjct: 876 EVLGRVYGVVSKRRGRVIDEEMK---EGTP-FFIVKAL 909 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,680,506 Number of Sequences: 5004 Number of extensions: 54528 Number of successful extensions: 183 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 183 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 311890690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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