SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060282.seq
         (678 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g19006.1 68417.m02801 26S proteasome regulatory subunit, puta...    79   2e-15
At5g45620.2 68418.m05607 26S proteasome regulatory subunit, puta...    77   8e-15
At5g45620.1 68418.m05608 26S proteasome regulatory subunit, puta...    77   8e-15
At3g46160.1 68416.m04995 protein kinase-related contains eukaryo...    30   1.2  
At3g43320.1 68416.m04579 hypothetical protein                          29   3.7  
At2g20800.1 68415.m02446 pyridine nucleotide-disulphide oxidored...    28   4.9  
At1g62780.1 68414.m07086 expressed protein                             28   4.9  
At5g41990.1 68418.m05112 protein kinase family protein contains ...    27   8.6  
At2g26900.1 68415.m03227 bile acid:sodium symporter family prote...    27   8.6  

>At4g19006.1 68417.m02801 26S proteasome regulatory subunit,
           putative (RPN9) similar to 26S proteasome subunit p40.5
           [Homo sapiens] gi|3618343|dbj|BAA33214
          Length = 386

 Score = 79.4 bits (187), Expect = 2e-15
 Identities = 36/70 (51%), Positives = 48/70 (68%)
 Frame = +1

Query: 46  FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLYNNFLTT 225
           +L  ++ + P L   +  L +LY KKLWHQLTLKL++F+     Q GD LIQLYNNF+T 
Sbjct: 6   YLESQKNAHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALSVFQAGDALIQLYNNFITD 65

Query: 226 FESKINPLSL 255
           FE++IN L L
Sbjct: 66  FETRINLLKL 75


>At5g45620.2 68418.m05607 26S proteasome regulatory subunit,
           putative (RPN9) contains similarity to 26S proteasome
           subunit p40.5 GI:3618343 from [Homo sapiens]
          Length = 350

 Score = 77.4 bits (182), Expect = 8e-15
 Identities = 37/85 (43%), Positives = 51/85 (60%)
 Frame = +1

Query: 46  FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLYNNFLTT 225
           +L   + + P L   +  L +LY KKLWHQLTLKL++F+     Q GD LIQ Y+NF+T 
Sbjct: 6   YLESLKNTHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALAVFQAGDALIQFYHNFITD 65

Query: 226 FESKINPLSLLRSLLISWNSMSTRE 300
           FE+KIN L L    ++     S +E
Sbjct: 66  FETKINLLKLAHFAVVVSRQYSEKE 90


>At5g45620.1 68418.m05608 26S proteasome regulatory subunit,
           putative (RPN9) contains similarity to 26S proteasome
           subunit p40.5 GI:3618343 from [Homo sapiens]
          Length = 386

 Score = 77.4 bits (182), Expect = 8e-15
 Identities = 37/85 (43%), Positives = 51/85 (60%)
 Frame = +1

Query: 46  FLTKKQASEPALAADWAKLEELYNKKLWHQLTLKLQEFVKNPALQRGDNLIQLYNNFLTT 225
           +L   + + P L   +  L +LY KKLWHQLTLKL++F+     Q GD LIQ Y+NF+T 
Sbjct: 6   YLESLKNTHPELGEWYNSLADLYQKKLWHQLTLKLEQFIALAVFQAGDALIQFYHNFITD 65

Query: 226 FESKINPLSLLRSLLISWNSMSTRE 300
           FE+KIN L L    ++     S +E
Sbjct: 66  FETKINLLKLAHFAVVVSRQYSEKE 90


>At3g46160.1 68416.m04995 protein kinase-related contains eukaryotic
           protein kinase domain, INTERPRO:IPR000719
          Length = 393

 Score = 30.3 bits (65), Expect = 1.2
 Identities = 14/59 (23%), Positives = 31/59 (52%)
 Frame = +3

Query: 225 LRKQNKSPIIVEIIAHIVEQYVNKRDAVTFLEKVETKVKMNDEALALCKVLQGQIYIEQ 401
           + K  K P++ ++  + +++Y + R+  T +EK   K+  +D      ++L G  YI +
Sbjct: 129 IEKDGKDPLLEKMYYNTLQEYSSGRNLATHIEKNRGKLPEDDVRSLANEILLGLKYIHE 187


>At3g43320.1 68416.m04579 hypothetical protein
          Length = 510

 Score = 28.7 bits (61), Expect = 3.7
 Identities = 17/39 (43%), Positives = 20/39 (51%)
 Frame = -1

Query: 615 DISTFSGR*ASPPSAHPTYRSAAR*YGSGTTDSVIFEAN 499
           DIS   GR   P S+HPT +   R  GS T   VI + N
Sbjct: 454 DISGVGGRNRCPKSSHPTRKQQPR--GSKTAHKVIPQGN 490


>At2g20800.1 68415.m02446 pyridine nucleotide-disulphide
           oxidoreductase family protein similar to GI:3718005
           alternative NADH-dehydrogenase {Yarrowia lipolytica} ;
           contains Pfam profile PF00070: Pyridine
           nucleotide-disulphide oxidoreductase
          Length = 582

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 10/28 (35%), Positives = 19/28 (67%)
 Frame = +1

Query: 145 KLQEFVKNPALQRGDNLIQLYNNFLTTF 228
           K+QEF K   L+ GD+++ +++  +T F
Sbjct: 259 KVQEFTKITLLEAGDHILNMFDKRITAF 286


>At1g62780.1 68414.m07086 expressed protein
          Length = 237

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 18/75 (24%), Positives = 36/75 (48%)
 Frame = +3

Query: 240 KSPIIVEIIAHIVEQYVNKRDAVTFLEKVETKVKMNDEALALCKVLQGQIYIEQLNDYDA 419
           +S ++ +  A ++E +++        E    + ++NDE  A  ++  GQI        D 
Sbjct: 81  QSRVVPDTAAELIEYFLDTEAQEIEYEIARLRGRLNDEFFAQIRLEIGQIRFAVTKTADI 140

Query: 420 AEKIIEHLESHSKML 464
            +++IE LE+  K L
Sbjct: 141 EDRLIE-LETLQKAL 154


>At5g41990.1 68418.m05112 protein kinase family protein contains
           protein kinase domain, Pfam:PF00069
          Length = 563

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 13/44 (29%), Positives = 23/44 (52%)
 Frame = +3

Query: 366 CKVLQGQIYIEQLNDYDAAEKIIEHLESHSKMLMV*LQFTDDFI 497
           C+++    Y+E       AE+++E L   S+ ++V     DDFI
Sbjct: 375 CRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFI 418


>At2g26900.1 68415.m03227 bile acid:sodium symporter family protein
           low similarity to SP|Q12908 Ileal sodium/bile acid
           cotransporter {Homo sapiens}; contains Pfam profile
           PF01758: Sodium Bile acid symporter family
          Length = 409

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 15/33 (45%), Positives = 22/33 (66%)
 Frame = -3

Query: 352 SSFILTFVSTFSRNVTASLLLTYCSTI*AMIST 254
           +S + T++S    NV  S+L+T CSTI A+I T
Sbjct: 204 ASNVATYIS--KGNVALSVLMTTCSTIGAIIMT 234


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,994,650
Number of Sequences: 28952
Number of extensions: 285995
Number of successful extensions: 717
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 701
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -