Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060281.seq
(580 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 198 4e-52
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 116 2e-27
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 103 2e-23
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 100 3e-22
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 68 9e-13
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 66 5e-12
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 56 5e-09
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 42 7e-05
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 30 0.28
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 27 2.6
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 27 2.6
SPBC3D6.10 |apn2||AP-endonuclease Apn2|Schizosaccharomyces pombe... 27 2.6
SPAC328.05 |||RNA-binding protein involved in export of mRNAs|Sc... 26 3.5
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 198 bits (484), Expect = 4e-52
Identities = 102/152 (67%), Positives = 108/152 (71%)
Frame = +3
Query: 78 DEEVAALVVDNGSGMXKAGFARR*CSSRRVPLDRGXAPPSGRDGRYGTEGLLCRR*GTEK 257
+EE+AALV+DNGSGM KAGFA P G G G + K
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 258 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQ 437
RGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVAPEEHP LLTE PLNPK+NREKMTQ
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 438 IMFETFNTXAMYVAIQAVLSLYAXVVPTGIVL 533
I+FETFN A YVAIQAVLSLYA TGIVL
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVL 153
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 116 bits (280), Expect = 2e-27
Identities = 57/152 (37%), Positives = 93/152 (61%), Gaps = 2/152 (1%)
Frame = +3
Query: 84 EVAALVVDNGSGMXKAGFARR*CSSRRVP--LDRGXAPPSGRDGRYGTEGLLCRR*GTEK 257
E A +V+DNG+G K G+A+ + P + R + G + ++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 258 RGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQ 437
R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM +
Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121
Query: 438 IMFETFNTXAMYVAIQAVLSLYAXVVPTGIVL 533
MFE + +YVAIQAVLSLYA + +G+V+
Sbjct: 122 TMFERYGFGGVYVAIQAVLSLYAQGLSSGVVV 153
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 103 bits (247), Expect = 2e-23
Identities = 57/147 (38%), Positives = 79/147 (53%), Gaps = 1/147 (0%)
Frame = +3
Query: 96 LVVDNGSGMXKAGFARR*CSSRRVPLDRGXAPPSGRDGRYGTEGLLCRR*GTEKRGILTL 275
+ +DNGSG KAGFA P G + + RG+L +
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71
Query: 276 KYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEHPVLLTEXPLNPKANREKMTQIMFET 452
+ PIE GI+ NW DME+IW + + ++ L PEEHP+LLTE PL N+EK+ + +ET
Sbjct: 72 QRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYFYET 131
Query: 453 FNTXAMYVAIQAVLSLYAXVVPTGIVL 533
N A+ ++Q VL+LYA TGIVL
Sbjct: 132 LNVPALSFSLQPVLALYASARTTGIVL 158
Score = 44.4 bits (100), Expect = 1e-05
Identities = 19/37 (51%), Positives = 25/37 (67%)
Frame = +1
Query: 142 GDDAPRAVFPSIVGXPRHQGVMVGMGQKDSYVGDEAQ 252
GDD P+ +FP+ VG +H+ VM QKD +VG EAQ
Sbjct: 27 GDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQ 63
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 99.5 bits (237), Expect = 3e-22
Identities = 42/79 (53%), Positives = 54/79 (68%)
Frame = +3
Query: 270 TLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQIMFE 449
+L YPI HG + NWD ME+ W + + LR PE+H LLTE PLNP NRE +IMFE
Sbjct: 89 SLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENRENTAEIMFE 148
Query: 450 TFNTXAMYVAIQAVLSLYA 506
+FN +Y+A+QAVL+L A
Sbjct: 149 SFNCAGLYIAVQAVLALAA 167
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 68.1 bits (159), Expect = 9e-13
Identities = 45/142 (31%), Positives = 67/142 (47%)
Frame = +3
Query: 81 EEVAALVVDNGSGMXKAGFARR*CSSRRVPLDRGXAPPSGRDGRYGTEGLLCRR*GTEKR 260
+EV+A+V+D GS + GF+ +P G R +G E + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRR--LFGEEYIYKSNPGME-- 64
Query: 261 GILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQI 440
+K I +G V NWD +W + +L+ P EHP+L+TE NP NR K +
Sbjct: 65 ----IKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLET 120
Query: 441 MFETFNTXAMYVAIQAVLSLYA 506
MFE+ A Y+A Q + +A
Sbjct: 121 MFESLRCPATYLAKQETCAAFA 142
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 65.7 bits (153), Expect = 5e-12
Identities = 45/151 (29%), Positives = 74/151 (49%), Gaps = 3/151 (1%)
Frame = +3
Query: 96 LVVDNGSGMXKAGFARR*CSSRRVPLDRGXAPPSGRDGRYGTEGLLCRR*GTEKRGILTL 275
LV+DNGS +AG+ ++ D + RD + L + G ++
Sbjct: 27 LVIDNGSWQLRAGWGGE--KDPKLVFDNLVS--RYRDRKLSRTSTLVGNDTLIEVGSRSI 82
Query: 276 -KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQIMFET 452
+ P E +++NWD ME++ +TF +L + EHP+ +TE NP R MT+++FE
Sbjct: 83 ARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVRSTMTELLFEL 141
Query: 453 FNTXAMYVAIQAVLSLYAXVVP--TGIVLGL 539
+N ++ I + S Y P +GIVL L
Sbjct: 142 YNAPSVAYGIDGLFSFYHNTKPSSSGIVLNL 172
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 55.6 bits (128), Expect = 5e-09
Identities = 37/153 (24%), Positives = 70/153 (45%)
Frame = +3
Query: 81 EEVAALVVDNGSGMXKAGFARR*CSSRRVPLDRGXAPPSGRDGRYGTEGLLCRR*GTEKR 260
EE+ +LV+D GS + G+A +P G +Y + L
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYGVRSDVTGRNKYVVDELQIH---APIP 64
Query: 261 GILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEXPLNPKANREKMTQI 440
G + +K +GI+ +W+ W +L+V P E+ +++TE NP++ R+++ +
Sbjct: 65 G-MEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEA 123
Query: 441 MFETFNTXAMYVAIQAVLSLYAXVVPTGIVLGL 539
FE + A Y+ QAV +A T +++ +
Sbjct: 124 AFEQLHVPAFYLTKQAVCVAFANSKSTALIVDI 156
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 41.9 bits (94), Expect = 7e-05
Identities = 25/90 (27%), Positives = 42/90 (46%), Gaps = 4/90 (4%)
Frame = +3
Query: 282 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEXPLNPKANREKMTQIMFE 449
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 450 TFNTXAMYVAIQAVLSLYAXVVPTGIVLGL 539
+A + ++ LYA + G+V+ +
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDI 201
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 29.9 bits (64), Expect = 0.28
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = -1
Query: 256 FSVPHLLHKSPSVPYRPSRPDGGAXPRSRGTR 161
+ + L+ K+PS PY SRP A S TR
Sbjct: 304 YDIDQLISKAPSYPYSSSRPSASASLASSPTR 335
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 26.6 bits (56), Expect = 2.6
Identities = 13/42 (30%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = +3
Query: 342 FYNELRVAPEEHPVLLTE--XPLNPKANREKMTQIMFETFNT 461
+Y L E+HP+LLT+ L P+ + ++ +I ++ NT
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENT 1440
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 26.6 bits (56), Expect = 2.6
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +1
Query: 142 GDDAPRAVFPSIVGXPRHQGVMVGMGQKDSYVGDE 246
G+ PRA F ++ P H G+++ M KD G+E
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEE 534
>SPBC3D6.10 |apn2||AP-endonuclease Apn2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 523
Score = 26.6 bits (56), Expect = 2.6
Identities = 20/65 (30%), Positives = 29/65 (44%)
Frame = -1
Query: 229 SPSVPYRPSRPDGGAXPRSRGTRREEHHLLAKPAXHIPEPLSTTNAATSSSHILNX*N** 50
SP+V S D A P + R+E+ LL+ A E T S +L+ N
Sbjct: 371 SPTVSRANSVIDVDAYPPEKRRRKEQSKLLSFFAKQKEEKEETNKTEDVSIEVLDNNNES 430
Query: 49 DVGVT 35
D+G+T
Sbjct: 431 DIGLT 435
>SPAC328.05 |||RNA-binding protein involved in export of
mRNAs|Schizosaccharomyces pombe|chr 1|||Manual
Length = 434
Score = 26.2 bits (55), Expect = 3.5
Identities = 12/30 (40%), Positives = 15/30 (50%)
Frame = -1
Query: 295 PCSMGYLRVRIPLFSVPHLLHKSPSVPYRP 206
P + Y PL +VP +L PSV Y P
Sbjct: 349 PLQLSYAHYATPLPAVPTVLSGLPSVAYTP 378
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,345,253
Number of Sequences: 5004
Number of extensions: 46788
Number of successful extensions: 168
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 127
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 248115846
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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