Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060279.seq
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g45310.1 68418.m05562 expressed protein 35 0.043
At2g44400.1 68415.m05522 DC1 domain-containing protein similar ... 30 1.2
At5g45790.1 68418.m05631 expressed protein ; expression support... 29 2.8
At5g07380.1 68418.m00845 hypothetical protein 29 2.8
At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138... 29 3.8
At2g25320.1 68415.m03029 meprin and TRAF homology domain-contain... 29 3.8
At1g24460.1 68414.m03081 myosin-related contains TIGRFAM TIGR016... 29 3.8
At4g21130.1 68417.m03055 transducin family protein / WD-40 repea... 28 5.0
At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A) 28 5.0
At2g22630.1 68415.m02682 MADS-box protein (AGL17) nearly identic... 28 6.6
At4g09570.1 68417.m01575 calcium-dependent protein kinase, putat... 27 8.7
At4g02120.1 68417.m00283 CTP synthase, putative / UTP--ammonia l... 27 8.7
>At5g45310.1 68418.m05562 expressed protein
Length = 352
Score = 35.1 bits (77), Expect = 0.043
Identities = 22/76 (28%), Positives = 42/76 (55%), Gaps = 5/76 (6%)
Frame = +2
Query: 248 EQHLRDRIKSK--VDEQFDQLEREYSD---KIDGFHDNIQYFKDEHYSVSCQNGSVLKSK 412
E++LR+ IK +++ D+LE E+ + KI+ +Q K+E+ + NG +SK
Sbjct: 111 EKNLREAIKEYRIMEQDLDELEDEHDEAISKIEKLEAELQELKEENLQLMEVNGKDYRSK 170
Query: 413 FAKILKSHDYTDKKSI 460
K+ S + ++ +SI
Sbjct: 171 KGKVKPSEEPSEIRSI 186
>At2g44400.1 68415.m05522 DC1 domain-containing protein similar to
GP|2435515|AF024504
Length = 146
Score = 30.3 bits (65), Expect = 1.2
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = -1
Query: 566 TWLLPFXNPGFNTHTAT*QCCVCRPIGANS 477
T+ +P PG + ++ QCCVCR +G+NS
Sbjct: 103 TFSMPPGGPG-SAAASSFQCCVCRGLGSNS 131
>At5g45790.1 68418.m05631 expressed protein ; expression supported
by MPSS
Length = 423
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 2/37 (5%)
Frame = +3
Query: 66 CKMCVIFPVEIDVSQTVIRDCHVD--EQTRELVYINK 170
CK+ F V ID + V+R+C VD ++ ++L+ ++K
Sbjct: 129 CKITEKFKVLIDEEENVVRECEVDAVKRVKKLLLLSK 165
>At5g07380.1 68418.m00845 hypothetical protein
Length = 595
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/42 (30%), Positives = 20/42 (47%)
Frame = +1
Query: 517 VAVCVLKPGFXNGSNQVLSFEYNPIGNKVIVPVAHEINDTXL 642
+ +C + +G++Q L F Y P GN + H IN L
Sbjct: 45 IGLCYILKDRISGNDQQLKFAYTPTGNFCLRDFHHAINSLPL 86
>At4g24530.1 68417.m03516 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown; previously annotated as
'PsRT17-1 like protein' based on similarity to PsRT17-1
(GP:1778376) [Pisum sativum] which was based upon
similarity to axi 1 protein (GB:X80301) (GI:559920) from
[Nicotiana tabacum], which, due to scienitific fraud was
retracted. Retraction in: Schell J. EMBO J 1999 May
17;18(10):2908. PMID:10400497.
Length = 519
Score = 28.7 bits (61), Expect = 3.8
Identities = 18/43 (41%), Positives = 20/43 (46%)
Frame = -1
Query: 407 FSTRCHFGNLPNSVHL*NTGCYREIHQFYRCIRVLVDQIVHRL 279
FS R F NLP S+ E F IR L D +VHRL
Sbjct: 226 FSHRLAFDNLPESIQRLRCKVNFEALNFVPHIRELGDALVHRL 268
>At2g25320.1 68415.m03029 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein weak similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 1663
Score = 28.7 bits (61), Expect = 3.8
Identities = 11/40 (27%), Positives = 23/40 (57%)
Frame = +2
Query: 224 SYTKRYAQEQHLRDRIKSKVDEQFDQLEREYSDKIDGFHD 343
S T++ ++ + R+KS++ + D+ RE D ++ F D
Sbjct: 1376 SITQKLSESEAANTRLKSEMKAEADRFSREKKDLVEQFRD 1415
>At1g24460.1 68414.m03081 myosin-related contains TIGRFAM TIGR01612:
reticulocyte binding protein; similar to Myosin heavy
chain, non-muscle (Zipper protein) (Myosin II)
(SP:Q99323) {Drosophila melanogaster} similar to EST
gb|T76116
Length = 1730
Score = 28.7 bits (61), Expect = 3.8
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +2
Query: 248 EQHLRDRIKSKVDEQFDQLEREYSDKIDGFHDNIQYFK 361
E H I VD FD++E++ D D + N + F+
Sbjct: 617 EDHTSSDINLLVDRSFDKIEKQIRDSSDSSYGNEEIFE 654
>At4g21130.1 68417.m03055 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400); some
similarity to a group of proteins with homology to
mammalian apoptosis regulators identified in zebrafish
(PUBMED:10917738)Apaf-1(gi:7677507)
Length = 537
Score = 28.3 bits (60), Expect = 5.0
Identities = 15/33 (45%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
Frame = +3
Query: 123 DCHV---DEQTRELVYINKIMNTQLTKPVLMMF 212
DCHV D +TRE V +NK+ N Q+ V F
Sbjct: 227 DCHVHLWDIRTREHVQVNKLKNNQVKDSVHCAF 259
>At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A)
Length = 135
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = +3
Query: 483 CPNWSTNTTLLRG 521
CP WS NT LLRG
Sbjct: 76 CPTWSENTVLLRG 88
>At2g22630.1 68415.m02682 MADS-box protein (AGL17) nearly identical
to MADS-box protein AGL17 [Arabidopsis thaliana]
GI:862648
Length = 227
Score = 27.9 bits (59), Expect = 6.6
Identities = 18/61 (29%), Positives = 27/61 (44%)
Frame = -1
Query: 227 RTRNVKHHENGFCQLRVHNLVNVHQLSGLFVHMTISNNRLGHVDFYRKNYTHFAMVKLKC 48
R RN+ HHEN +V + + + T + N LGH + Y A V+L+
Sbjct: 154 RKRNLVHHENLELSRKVQRIHQENVELYKKAYGTSNTNGLGHHELVDAVYESHAQVRLQL 213
Query: 47 S 45
S
Sbjct: 214 S 214
>At4g09570.1 68417.m01575 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Arabidopsis thaliana] gi|604881|dbj|BAA04830;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 501
Score = 27.5 bits (58), Expect = 8.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 422 ILKSHDYTDKKSIETYEKYCLPQLVDKH 505
I K D + KK I +E C P +VD+H
Sbjct: 260 IYKMLDRSPKKRISAHEALCHPWIVDEH 287
>At4g02120.1 68417.m00283 CTP synthase, putative / UTP--ammonia
ligase, putative similar to SP|P17812 CTP synthase (EC
6.3.4.2) (UTP--ammonia ligase) {Homo sapiens}; contains
Pfam profile PF00117: glutamine amidotransferase class-I
Length = 556
Score = 27.5 bits (58), Expect = 8.7
Identities = 17/76 (22%), Positives = 33/76 (43%), Gaps = 1/76 (1%)
Frame = -1
Query: 290 VHRLLILFCRANVVLARNASYRTRNVKHHENGFCQLRVHNLVNVHQLSGLF-VHMTISNN 114
V L L + + R+A + K + FC + N++N+H + ++ V + + N
Sbjct: 201 VRELRALGLTPHFLACRSAQPLLESTKAKLSQFCHVAAANILNIHDVPNIWHVPLLLRNQ 260
Query: 113 RLGHVDFYRKNYTHFA 66
H + N T+ A
Sbjct: 261 NAHHSILKQLNLTNVA 276
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,502,619
Number of Sequences: 28952
Number of extensions: 273901
Number of successful extensions: 804
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 786
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 804
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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