BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060278.seq (670 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual 50 2e-07 SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual 43 3e-05 SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated memb... 40 2e-04 SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces po... 34 0.016 SPBC31F10.07 |||cortical component Lsb5 |Schizosaccharomyces pom... 27 1.8 SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha... 27 2.4 SPAC19A8.03 |||phosphatidylinositol-3-phosphatase |Schizosacchar... 27 2.4 SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces po... 27 3.2 SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 26 4.3 SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 26 5.6 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 5.6 SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 25 7.5 SPBC660.10 |||translation elongation factor G|Schizosaccharomyce... 25 7.5 >SPBC25H2.16c |||adaptin|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 50.4 bits (115), Expect = 2e-07 Identities = 25/61 (40%), Positives = 38/61 (62%), Gaps = 2/61 (3%) Frame = +2 Query: 260 TVVMYTLTVLETCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTV--VQDKVLSLIQC 433 TV L +L+ CVKNCG F + + +KEF++ELV+ P+ P + +Q +LSLI+ Sbjct: 60 TVAYLALNLLDICVKNCGYAFRLQIASKEFLNELVRRF-PERPPSRLNKIQVMILSLIEE 118 Query: 434 W 436 W Sbjct: 119 W 119 >SPAC1F3.05 |||adaptin |Schizosaccharomyces pombe|chr 1|||Manual Length = 510 Score = 43.2 bits (97), Expect = 3e-05 Identities = 22/62 (35%), Positives = 34/62 (54%), Gaps = 3/62 (4%) Frame = +2 Query: 260 TVVMYTLTVLETCVKNCGKPFHVLVCNKEFISELVKLIGPKNDP---PTVVQDKVLSLIQ 430 TV L +L+ CVKNCG PFH + ++EF++ V N P +Q K+L +++ Sbjct: 59 TVSYLALHLLDICVKNCGYPFHFQIASEEFLNGFVSRF--PNHPISRMNKIQSKMLEMLE 116 Query: 431 CW 436 W Sbjct: 117 EW 118 Score = 33.5 bits (73), Expect = 0.028 Identities = 22/60 (36%), Positives = 33/60 (55%), Gaps = 1/60 (1%) Frame = +3 Query: 90 STPVGQKIEQATDGALPSENWALNMEICDIINSST-DGPKDAIKAIRKRLTTSAGKTTQW 266 S + + I++ATD N ALN+EI D+IN + P++A I KR+ SA T + Sbjct: 4 SQTLSKYIDKATDQFNLEPNLALNIEIADLINEKKGNTPREAALLILKRV-NSANPTVSY 62 >SPAC19A8.05c |vps27|sst4|sorting receptor for ubiquitinated membrane proteins, ESCRT 0 complex|Schizosaccharomyces pombe|chr 1|||Manual Length = 610 Score = 40.3 bits (90), Expect = 2e-04 Identities = 28/103 (27%), Positives = 48/103 (46%), Gaps = 1/103 (0%) Frame = +2 Query: 224 KKKVDYKRWEDYTVVMYTLTVLETCVKNCGKPFHVLVCNKEFISELVKLIGPKNDPPTVV 403 K ++D+ + V + L + +TCVKN G F + + ++EF+ LV ++ V Sbjct: 55 KSRIDHS---NPNVQIMALKLTDTCVKNGGSGFLLEIASREFMDNLVSILRSPAGIDEDV 111 Query: 404 QDKVLSLIQCWADAF-QNQAELQGVGQVYNELRTKGLNFR*PT 529 + +L IQ WA A + L + VY L+ F P+ Sbjct: 112 KMVILRYIQSWALAVPDTNSPLSYIIHVYQNLKDGDYEFPEPS 154 >SPBC1734.08 |hse1||STAM like protein Hse1|Schizosaccharomyces pombe|chr 2|||Manual Length = 373 Score = 34.3 bits (75), Expect = 0.016 Identities = 19/46 (41%), Positives = 28/46 (60%), Gaps = 1/46 (2%) Frame = +3 Query: 111 IEQATDGALPSENWALNMEICDIINS-STDGPKDAIKAIRKRLTTS 245 I QATD E W + M+ CD ++S S D +++IK + KRL T+ Sbjct: 13 ILQATDEKNTKEKWDVIMDACDQLSSTSGDVGRNSIKFLNKRLDTA 58 >SPBC31F10.07 |||cortical component Lsb5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 304 Score = 27.5 bits (58), Expect = 1.8 Identities = 13/51 (25%), Positives = 28/51 (54%) Frame = +3 Query: 93 TPVGQKIEQATDGALPSENWALNMEICDIINSSTDGPKDAIKAIRKRLTTS 245 T V I++ T E+ + +++ + +N + GP++A + +RK+L S Sbjct: 11 TAVTTYIDRLTSRDTDDEDLSGIVQLSEAVNLTVTGPREASRTLRKKLKYS 61 >SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccharomyces pombe|chr 2|||Manual Length = 485 Score = 27.1 bits (57), Expect = 2.4 Identities = 15/44 (34%), Positives = 23/44 (52%) Frame = -1 Query: 352 YKFLIADENMERLSTVFYACFQDSQRVHHHCVVFPALVVNLFLI 221 Y+ + DE R T +YA +SQ+VH +F L +N +I Sbjct: 243 YEINLMDELTLRGVTQYYAFVDESQKVHCLNTLFSKLQINQSII 286 >SPAC19A8.03 |||phosphatidylinositol-3-phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 559 Score = 27.1 bits (57), Expect = 2.4 Identities = 11/26 (42%), Positives = 13/26 (50%) Frame = -3 Query: 272 TSPLCSLPSACSQPFSYCFNGIFRSV 195 TS LC+LP C P+ G F V Sbjct: 349 TSQLCALPQLCLDPYYRTIEGFFALV 374 >SPAC57A7.11 |mip1||WD repeat protein Mip1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1313 Score = 26.6 bits (56), Expect = 3.2 Identities = 11/34 (32%), Positives = 18/34 (52%), Gaps = 2/34 (5%) Frame = +2 Query: 386 DPPTVVQDKVLSLIQCWADAFQNQAE--LQGVGQ 481 DPP V++ + +CW D F A L+ +G+ Sbjct: 130 DPPDVIKPNPAAKYECWIDPFSLPASKALEAIGK 163 >SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 26.2 bits (55), Expect = 4.3 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = -1 Query: 460 SLVLEGIGPTLDEAQYF 410 SL+ EG+ PTLDE F Sbjct: 433 SLITEGVNPTLDEVSKF 449 >SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 2363 Score = 25.8 bits (54), Expect = 5.6 Identities = 12/19 (63%), Positives = 13/19 (68%) Frame = +1 Query: 85 PFLRRWVKKLNKPQMAHYH 141 P RRW KLN PQMA+ H Sbjct: 242 PTYRRW--KLNLPQMANLH 258 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 25.8 bits (54), Expect = 5.6 Identities = 14/39 (35%), Positives = 23/39 (58%) Frame = +2 Query: 341 KEFISELVKLIGPKNDPPTVVQDKVLSLIQCWADAFQNQ 457 + F+ +L KLI +P + +Q +LSLI+ A A Q + Sbjct: 884 RSFLPDLFKLIKDFWNPHSNLQFTILSLIESLARAMQGE 922 >SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharomyces pombe|chr 2|||Manual Length = 733 Score = 25.4 bits (53), Expect = 7.5 Identities = 11/30 (36%), Positives = 20/30 (66%) Frame = +3 Query: 108 KIEQATDGALPSENWALNMEICDIINSSTD 197 KI +D A+PS ++A N+ +I+++TD Sbjct: 640 KISTVSDTAVPSMSFANNISSRSVISAATD 669 >SPBC660.10 |||translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 813 Score = 25.4 bits (53), Expect = 7.5 Identities = 12/32 (37%), Positives = 16/32 (50%) Frame = -3 Query: 122 GLFNFLTHRRRKGIPYTKKRHFCKIIKYLSID 27 G+ L+H G P T + FC I +LS D Sbjct: 608 GIVAGLSHGPLHGFPLTNLQSFCTISSFLSND 639 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,130,149 Number of Sequences: 5004 Number of extensions: 72054 Number of successful extensions: 203 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 196 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 203 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 305854096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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