Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060276.seq
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual 38 0.001
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 33 0.038
SPBC28E12.03 |rga4||GTPase activating protein Rga4|Schizosacchar... 31 0.16
SPAC29A4.11 |rga3||GTPase activating protein Rga3|Schizosaccharo... 28 1.1
SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces po... 28 1.1
SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyc... 27 1.9
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 25 7.7
SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyce... 25 7.7
>SPBC4F6.12 |||LIM domain|Schizosaccharomyces pombe|chr 2|||Manual
Length = 438
Score = 37.9 bits (84), Expect = 0.001
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = +1
Query: 295 RQWHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATRCVKC 432
++ H +CF C C + F RE + YC Y ++F+ RC C
Sbjct: 276 KKLHPQCFKCDTCSQNLEHVGFYYREGKFYCHLDYHEQFSPRCKHC 321
Score = 37.1 bits (82), Expect = 0.002
Identities = 22/74 (29%), Positives = 31/74 (41%), Gaps = 3/74 (4%)
Frame = +1
Query: 232 EMFVRRSK--KTVAGTKKMEYKTRQWHEKCFCCVVCKNPIGTK-SFIPREQEIYCAGCYE 402
E F R K KT + + +HE C C I R+ +C CY+
Sbjct: 312 EQFSPRCKHCKTPIEDQAVHINNDWFHENHHFCAGCSEVFNVNIPCIYRDDLYWCQTCYD 371
Query: 403 DKFATRCVKCNKII 444
+K+A +C KC K I
Sbjct: 372 NKYAVKCKKCRKPI 385
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 33.1 bits (72), Expect = 0.038
Identities = 15/48 (31%), Positives = 24/48 (50%), Gaps = 1/48 (2%)
Frame = +1
Query: 295 RQWHEKCFCCVVCKNPIGTK-SFIPREQEIYCAGCYEDKFATRCVKCN 435
+++H + F C +C G S+ E ++YC Y FA RC C+
Sbjct: 197 KKFHIEHFTCSLCYTVFGPNDSYYEYEGKVYCHYHYSTLFAARCCGCD 244
>SPBC28E12.03 |rga4||GTPase activating protein
Rga4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 933
Score = 31.1 bits (67), Expect = 0.16
Identities = 11/34 (32%), Positives = 20/34 (58%), Gaps = 1/34 (2%)
Frame = +2
Query: 458 VTYKNEPWHRECFTCTNATRR-SPASGSLSRDEK 556
V + + WH +CF C N ++ P+S S+D++
Sbjct: 37 VWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQ 70
Score = 30.7 bits (66), Expect = 0.20
Identities = 14/52 (26%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Frame = +1
Query: 256 KTVAGTKKMEYKTRQWHEKCFCCVVCKNPI--GTKSFIPREQ-EIYCAGCYE 402
++V T ++ + + WH CF CV C + ++ F +Q +I+C C +
Sbjct: 29 ESVPSTSQVWFGGKCWHSDCFKCVNCNKKLDPSSEDFSQDDQKQIFCKLCVD 80
>SPAC29A4.11 |rga3||GTPase activating protein
Rga3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 969
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/40 (27%), Positives = 20/40 (50%)
Frame = +1
Query: 301 WHEKCFCCVVCKNPIGTKSFIPREQEIYCAGCYEDKFATR 420
+H +CF C C+ I +F + I+C C + + +R
Sbjct: 97 YHRECFRCHDCRKQIIDSNFKRDNRTIFCNDCKQVRHPSR 136
Score = 27.9 bits (59), Expect = 1.4
Identities = 7/11 (63%), Positives = 8/11 (72%)
Frame = +1
Query: 301 WHEKCFCCVVC 333
WH+ CFCC C
Sbjct: 40 WHKDCFCCTKC 50
Score = 26.2 bits (55), Expect = 4.4
Identities = 6/20 (30%), Positives = 12/20 (60%)
Frame = +2
Query: 458 VTYKNEPWHRECFTCTNATR 517
+++ WH++CF CT +
Sbjct: 33 ISFGGHMWHKDCFCCTKCDK 52
>SPAC32A11.02c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 851
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +2
Query: 83 SRLHLTMKTVGQDRIRRARENNEDVAILSMFLPNATAERENKKFCCAL 226
S LH + K G+D R + E+ A+ ++ LP+ +R +K AL
Sbjct: 651 SNLHKSSKEKGKDSQVRVALHKENTALNNIVLPSGNLQRSEEKRRAAL 698
>SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1238
Score = 27.5 bits (58), Expect = 1.9
Identities = 15/41 (36%), Positives = 18/41 (43%), Gaps = 2/41 (4%)
Frame = +3
Query: 543 RATRSPYCGRVLRGNCFAKXCTSCTKPIYR--YPGGQPAFN 659
R TR+ Y + CF + CT KP YP P FN
Sbjct: 1110 RRTRNAYTQKSGEVECFMETCTPIAKPAPANWYPVPPPGFN 1150
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 25.4 bits (53), Expect = 7.7
Identities = 18/89 (20%), Positives = 38/89 (42%)
Frame = +2
Query: 29 KTKKKNKMSADVLSEDLKSRLHLTMKTVGQDRIRRARENNEDVAILSMFLPNATAERENK 208
K KK++K+ + E + L + +++ E + I + +P + E K
Sbjct: 165 KEKKESKVKLESKEEKISRNLRSSSRSISP-----VTEQPQSPKIQPV-IPEKKEKSEKK 218
Query: 209 KFCCALRERCLLGDPRKLLQEPRRWSTRP 295
+ LR+R + + + P+R +T P
Sbjct: 219 ESSMTLRKRSVSPSSQNTARSPKRMATEP 247
>SPAC4A8.05c |myp2|myo3|myosin II heavy chain |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2104
Score = 25.4 bits (53), Expect = 7.7
Identities = 14/60 (23%), Positives = 30/60 (50%)
Frame = +1
Query: 10 ATTVLSKDKKKKQNVSGRLE*RFEIPPSFDNEDSRTR*DKTSAGKQ*RRCDIIYVSSQRD 189
A + LSK ++ K ++GRL+ E+ F ++ + + A + + +++YV D
Sbjct: 1647 AESSLSKAERSKNYLTGRLQEVEELARGFQTTNADLQNELADAVVKQKEYEVLYVEKSND 1706
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,513,584
Number of Sequences: 5004
Number of extensions: 47243
Number of successful extensions: 151
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 138
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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