SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060274.seq
         (670 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac...    97   2e-21
SPAC1556.02c |sdh1||succinate dehydrogenase Sdh1|Schizosaccharom...    29   0.46 
SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein S15a...    29   0.80 
SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein S...    29   0.80 
SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi...    28   1.1  
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac...    28   1.4  
SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr...    27   2.4  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   4.3  
SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr ...    26   5.6  
SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit |...    26   5.6  
SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase Sen1|...    25   9.9  

>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
           Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 815

 Score = 97.1 bits (231), Expect = 2e-21
 Identities = 43/84 (51%), Positives = 59/84 (70%)
 Frame = +1

Query: 256 GDTVLLKGKRRKETVCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVVSIAPCPSVKYGKR 435
           GDTV++KGKRRK+TV IVL+D+   D   R+             D+V+I PCP +KY +R
Sbjct: 74  GDTVVVKGKRRKDTVLIVLTDEEMEDGVARINRVVRNNLRVRLGDIVTINPCPDIKYAER 133

Query: 436 VHILPIDDSVEGLTGNLFEVYLKP 507
           + +LP+ D+VEGLTG+LF+VYLKP
Sbjct: 134 ISVLPLADTVEGLTGSLFDVYLKP 157



 Score = 60.5 bits (140), Expect = 2e-10
 Identities = 28/52 (53%), Positives = 37/52 (71%)
 Frame = +2

Query: 98  NKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 253
           N   D  S +D +TAILR+K +PN L+V++A +DDNSV+ LS   ME LQLF
Sbjct: 21  NPPKDTYSAEDTATAILRKKRKPNSLVVDDATNDDNSVITLSSNTMETLQLF 72



 Score = 47.2 bits (107), Expect = 2e-06
 Identities = 21/32 (65%), Positives = 25/32 (78%)
 Frame = +3

Query: 498 LEAVFMEAYRPIHRDDTFMVRGGMRAVEFKVV 593
           L+  F+EAYRPI + D F+VRG MR VEFKVV
Sbjct: 155 LKPYFVEAYRPIRKGDLFVVRGSMRQVEFKVV 186


>SPAC1556.02c |sdh1||succinate dehydrogenase
           Sdh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 641

 Score = 29.5 bits (63), Expect = 0.46
 Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
 Frame = -3

Query: 611 GDGSVSDHFELDGAHAPAD--HEGVVTMDRTVSLHEDGFKYTSNRLPVRPSTESSIGSMW 438
           G G   DH  L  +H PA+   E +  +  T ++   G   T   +PV P+   ++G + 
Sbjct: 349 GVGPEKDHCYLQLSHLPAEILKERLPGISETAAIFA-GVDVTKEPIPVLPTVHYNMGGIP 407

Query: 437 TRF 429
           TRF
Sbjct: 408 TRF 410


>SPAC5D6.01 |rps2202|rps22-2, rps15a-2|40S ribosomal protein
           S15a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 130

 Score = 28.7 bits (61), Expect = 0.80
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -1

Query: 466 QLNHQLAVCGLV---FHISLKDTERWI 395
           QLN ++  CG++   F++ LKD E+W+
Sbjct: 64  QLNGRINKCGVISPRFNVKLKDIEKWV 90


>SPAC22A12.04c |rps2201|rps22-1, rps15a-1|40S ribosomal protein
           S15a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 130

 Score = 28.7 bits (61), Expect = 0.80
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 3/27 (11%)
 Frame = -1

Query: 466 QLNHQLAVCGLV---FHISLKDTERWI 395
           QLN ++  CG++   F++ LKD E+W+
Sbjct: 64  QLNGRINKCGVISPRFNVKLKDIEKWV 90


>SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 319

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 12/39 (30%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
 Frame = +3

Query: 123 LMIYRPRSSVARTDPTVSLSKKQSAMTTQSWH-FHRPKW 236
           L + R    +   DP ++ +  Q  + T+SWH FH  +W
Sbjct: 259 LALIRGCDGLLSDDPVMARALSQGPIVTKSWHYFHYSEW 297


>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
           E3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 905

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
 Frame = -1

Query: 436 LVFHISLKDTERWIP-HQKDAHEGCFSQHGSSEFSHQG 326
           LV  + LKD   WI    + + E  F   G ++FS+QG
Sbjct: 435 LVSDLYLKDLWSWIHLSHRQSEESLFGDTGDTDFSYQG 472


>SPCC285.10c |||SPRY domain protein|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 382

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 2/62 (3%)
 Frame = +3

Query: 72  FLDSNNKF*IKWQIIKALMIYRPRSSVARTDPTVS--LSKKQSAMTTQSWHFHRPKWSNF 245
           F+++N K   KW++  A+    P  S + + PT+S   + + SA TT     +RP  S  
Sbjct: 286 FIEANIK---KWRLAPAVGSLAPPPSYSTSQPTISWDAASESSAGTTTQGDTNRPDKSKN 342

Query: 246 NS 251
            S
Sbjct: 343 RS 344


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 15/56 (26%), Positives = 31/56 (55%)
 Frame = +2

Query: 95  LNKMADNKSPDDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLFLVT 262
           L  +A N   + L+T   +RK + N  ++ +A ++ N+  +++ A  E+ QL+  T
Sbjct: 768 LGSLAGNLPQESLTTKS-KRKSKVNLELMFDATAEKNNENSMNSAVFEKSQLYPFT 822


>SPBC428.15 |||GTP binding protein|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 409

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 11/27 (40%), Positives = 19/27 (70%), Gaps = 1/27 (3%)
 Frame = +2

Query: 122 PD-DLSTAILRRKDRPNRLIVEEAVSD 199
           PD D + + + RK+ PNRL++  A+S+
Sbjct: 233 PDADANISKIARKEDPNRLVLASAISE 259


>SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 661

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 14/36 (38%), Positives = 19/36 (52%)
 Frame = +2

Query: 233 MEQLQLFLVTQSCSRANAARKPFASCSQTIIALMRK 340
           + Q QLF   +  S  N  R+ F    QTI+ +MRK
Sbjct: 80  LTQNQLFKNVEDYSFYNLNREKFQRDKQTIVGVMRK 115


>SPAC6G9.10c |sen1||ATP-dependent 5' to 3' DNA/RNA helicase
           Sen1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1687

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = +3

Query: 468 GSYWQSIRSILEAVFMEAYR 527
           G+YWQ I +IL+ VF    R
Sbjct: 609 GAYWQCIWNILDLVFSNVAR 628


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,807,285
Number of Sequences: 5004
Number of extensions: 57186
Number of successful extensions: 175
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 175
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -