BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060274.seq (670 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_45627| Best HMM Match : AAA (HMM E-Value=0) 73 3e-13 SB_28772| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.4 SB_33107| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 6.0 >SB_45627| Best HMM Match : AAA (HMM E-Value=0) Length = 628 Score = 72.5 bits (170), Expect = 3e-13 Identities = 33/43 (76%), Positives = 40/43 (93%) Frame = +2 Query: 125 DDLSTAILRRKDRPNRLIVEEAVSDDNSVVALSQAKMEQLQLF 253 D+L+TAIL+ K RPNRL+VEEAV+DDNSVV +SQAKME+LQLF Sbjct: 5 DELATAILKNKSRPNRLLVEEAVNDDNSVVTMSQAKMEELQLF 47 Score = 56.4 bits (130), Expect = 2e-08 Identities = 27/47 (57%), Positives = 31/47 (65%) Frame = +1 Query: 256 GDTVLLKGKRRKETVCIVLSDDNCPDEKIRMXXXXXXXXXXXXSDVV 396 GDTVL+KGK+RK+TVCIVLSDD D+KIRM DVV Sbjct: 49 GDTVLIKGKKRKDTVCIVLSDDTISDDKIRMNRVVRMNLRVRLGDVV 95 Score = 34.3 bits (75), Expect = 0.091 Identities = 14/17 (82%), Positives = 16/17 (94%) Frame = +3 Query: 543 DTFMVRGGMRAVEFKVV 593 D F+VRGGMRAVEFKV+ Sbjct: 104 DMFLVRGGMRAVEFKVI 120 Score = 29.5 bits (63), Expect = 2.6 Identities = 11/13 (84%), Positives = 12/13 (92%) Frame = +1 Query: 595 ETDPSPFCIVAXD 633 ETDPSP+CIVA D Sbjct: 121 ETDPSPYCIVAPD 133 >SB_28772| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 255 Score = 29.1 bits (62), Expect = 3.4 Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 2/36 (5%) Frame = +2 Query: 521 LPSDPS*RHLHGPR--GHARRRVQSGPKQIHHHFAS 622 +P+DPS H H P H + + P+QIHH F S Sbjct: 95 IPTDPSHVHQHIPTDPSHVNKHI---PQQIHHMFTS 127 >SB_33107| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1079 Score = 28.3 bits (60), Expect = 6.0 Identities = 13/38 (34%), Positives = 20/38 (52%) Frame = -1 Query: 658 GFTVTVIXDQXPRCKMVMDLFRTTLNSTARMPPRTMKV 545 G T + RCK++M ++ TL + RMP +T V Sbjct: 499 GVTRALTETVLSRCKIIMGFYQVTLKTLHRMPFKTSGV 536 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 21,565,324 Number of Sequences: 59808 Number of extensions: 447598 Number of successful extensions: 1296 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 1157 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1296 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1729817375 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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