Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060273.seq
         (619 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    50   1e-06
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    48   4e-06
At3g63500.2 68416.m07153 expressed protein                             30   1.1  
At4g25000.1 68417.m03587 alpha-amylase, putative / 1,4-alpha-D-g...    30   1.4  
At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase fa...    29   2.5  
At1g31540.1 68414.m03869 disease resistance protein (TIR-NBS-LRR...    28   4.3  
At1g30100.1 68414.m03679 9-cis-epoxycarotenoid dioxygenase, puta...    28   4.3  
At2g17010.1 68415.m01961 mechanosensitive ion channel domain-con...    28   5.7  
At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative ...    28   5.7  
At1g65780.1 68414.m07465 tRNA-splicing endonuclease positive eff...    27   7.5  
At1g19130.1 68414.m02379 expressed protein                             27   7.5  
At3g13760.1 68416.m01736 DC1 domain-containing protein contains ...    27   10.0 
At2g40230.1 68415.m04947 transferase family protein similar to t...    27   10.0 

>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 29/61 (47%), Positives = 33/61 (54%)
 Frame = +3

Query: 96  MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLTRPDQNS 275
           MS K EKP L G RIKTRKR+     DP  F DA+VQ      GDL+   K +   D N 
Sbjct: 1   MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDAVVQIYHDNAGDLELVAKSIESSDLNF 59

Query: 276 T 278
           T
Sbjct: 60  T 60



 Score = 33.9 bits (74), Expect = 0.087
 Identities = 14/33 (42%), Positives = 21/33 (63%)
 Frame = +2

Query: 266 SKLDYRRYGEVIFDVLIAGGLLLPGGSVSMDGE 364
           S L++ RYG++ F+V+  GG   PG   S +GE
Sbjct: 55  SDLNFTRYGDIFFEVIFIGGRTQPGTVKSDEGE 87


>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|P47823
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 48.4 bits (110), Expect = 4e-06
 Identities = 29/59 (49%), Positives = 32/59 (54%)
 Frame = +3

Query: 96  MSQKVEKPVLSGQRIKTRKRDEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLTRPDQN 272
           MS K EKP L G RIKTRKR+     DP  F DALVQ      GDL+   + L   D N
Sbjct: 1   MSSK-EKPTLGGTRIKTRKRNIAAPLDPAAFSDALVQIYLDNAGDLELVARSLESSDLN 58



 Score = 31.1 bits (67), Expect = 0.61
 Identities = 13/33 (39%), Positives = 20/33 (60%)
 Frame = +2

Query: 266 SKLDYRRYGEVIFDVLIAGGLLLPGGSVSMDGE 364
           S L++ RYG++ F+V+  GG    G   S +GE
Sbjct: 55  SDLNFSRYGDIFFEVVFIGGRTQTGSVKSDEGE 87


>At3g63500.2 68416.m07153 expressed protein
          Length = 1162

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 21/70 (30%), Positives = 28/70 (40%)
 Frame = -2

Query: 357 SIDTEPPGSSRPPAMSTSNMTSP*RR*SSFDPAELGTCRLRRDRHRPAPDPVPERRGNRS 178
           ++D EPP S RP + S     S   R    D A       RR  +R   +  P+ R +RS
Sbjct: 17  NVDGEPPNSDRPASSSHRGFFSGNNRDRGEDAAGFSRAFSRRRSNRDLDNHRPDARYHRS 76

Query: 177 GHTSLSHLFF 148
                S   F
Sbjct: 77  ESACFSRRAF 86


>At4g25000.1 68417.m03587 alpha-amylase, putative /
           1,4-alpha-D-glucan glucanohydrolase, putative similar to
           alpha-amylase from Vigna mungo SP|P17859, Ipomoea nil
           GI:21670851; contains Pfam profile PF00128: Alpha
           amylase, catalytic domain
          Length = 423

 Score = 29.9 bits (64), Expect = 1.4
 Identities = 14/35 (40%), Positives = 19/35 (54%)
 Frame = +3

Query: 156 DEKEKYDPNGFRDALVQGLERAGGDLDAAYKYLTR 260
           D K  YD N  R  L Q +E AGG +  A+ + T+
Sbjct: 238 DGKLDYDQNEHRSGLKQWIEEAGGGVLTAFDFTTK 272


>At3g58560.1 68416.m06527 endonuclease/exonuclease/phosphatase
           family protein similar to SP|P31384 Glucose-repressible
           alcohol dehydrogenase transcriptional effector (Carbon
           catabolite repressor protein 4) {Saccharomyces
           cerevisiae}; contains Pfam profile PF03372:
           Endonuclease/Exonuclease/phosphatase family
          Length = 602

 Score = 29.1 bits (62), Expect = 2.5
 Identities = 22/68 (32%), Positives = 29/68 (42%)
 Frame = -2

Query: 249 TCRLRRDRHRPAPDPVPERRGNRSGHTSLSHLFFWS*SVDPIILVFLPSDSYNILILTGY 70
           +C +   R  PAP P P R  + SG     HL     +  P+ +      SYNIL  T  
Sbjct: 205 SCTILTSRVIPAPSPSPRRLISISGTDVTGHL---DSNGRPLSMGTFTVLSYNILSDTYA 261

Query: 69  VGAFYSFC 46
               YS+C
Sbjct: 262 SSDIYSYC 269


>At1g31540.1 68414.m03869 disease resistance protein (TIR-NBS-LRR
           class), putative domain signature TIR-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 776

 Score = 28.3 bits (60), Expect = 4.3
 Identities = 16/48 (33%), Positives = 22/48 (45%), Gaps = 3/48 (6%)
 Frame = +3

Query: 162 KEKYDPNGFRDALVQGLERAGG---DLDAAYKYLTRPDQNSTTDAMAK 296
           K+ Y P GF D +V  +  AG     L+   KYL R D     D + +
Sbjct: 363 KQNYAPKGFEDLVVDVVRHAGNFPLGLNLLGKYLRRRDMEYWMDMLPR 410


>At1g30100.1 68414.m03679 9-cis-epoxycarotenoid dioxygenase,
           putative / neoxanthin cleavage enzyme, putative /
           carotenoid cleavage dioxygenase, putative similar to
           9-cis-epoxycarotenoid dioxygenase GI:6715257 from
           [Phaseolus vulgaris]
          Length = 589

 Score = 28.3 bits (60), Expect = 4.3
 Identities = 25/86 (29%), Positives = 41/86 (47%)
 Frame = -2

Query: 450 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMTSP*RR*SS 271
           +SFTNT  + R +S +S+++   +L   + PS     P    P     ++  +P +R +S
Sbjct: 32  VSFTNTKPRRRKLSANSVSDTPNLLNFPNYPS-----PNPIIPE--KDTSRWNPLQRAAS 84

Query: 270 FDPAELGTCRLRRDRHRPAPDPVPER 193
                  T  LRR+R +P P  V  R
Sbjct: 85  AALDFAETALLRRERSKPLPKTVDPR 110


>At2g17010.1 68415.m01961 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 779

 Score = 27.9 bits (59), Expect = 5.7
 Identities = 16/61 (26%), Positives = 27/61 (44%)
 Frame = +2

Query: 254 NSAGSKLDYRRYGEVIFDVLIAGGLLLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQ 433
           ++A +  D  R   V F+V  AGG  + G   S    S  +++     N+D   ++  E 
Sbjct: 164 STARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQLQEEEV 223

Query: 434 V 436
           V
Sbjct: 224 V 224


>At1g33240.1 68414.m04108 trihelix DNA-binding protein, putative
           similar to GTL1 [Arabidopsis thaliana] GI:2664198
          Length = 669

 Score = 27.9 bits (59), Expect = 5.7
 Identities = 15/54 (27%), Positives = 25/54 (46%)
 Frame = -2

Query: 450 ISFTNTCSKFRMVSISSLAEKMQVLVLGDSPSIDTEPPGSSRPPAMSTSNMTSP 289
           +S     S  R  +I SL +K+    +   PS+ ++PP   +PP   T  +  P
Sbjct: 321 MSQERAASASRDAAIISLIQKITGHTIQLPPSLSSQPPPPYQPPPAVTKRVAEP 374


>At1g65780.1 68414.m07465 tRNA-splicing endonuclease positive
           effector-related contains similarity to SEN1, a positive
           effector of tRNA-splicing endonuclease [Saccharomyces
           cerevisiae] gi|172574|gb|AAB63976
          Length = 1065

 Score = 27.5 bits (58), Expect = 7.5
 Identities = 10/22 (45%), Positives = 13/22 (59%)
 Frame = +3

Query: 453 VGTSTWKKCFEEEMKKVSGYLK 518
           +  S WK CF +E KK  G +K
Sbjct: 861 LNNSKWKLCFSDEFKKYVGEIK 882


>At1g19130.1 68414.m02379 expressed protein
          Length = 187

 Score = 27.5 bits (58), Expect = 7.5
 Identities = 15/51 (29%), Positives = 24/51 (47%)
 Frame = +2

Query: 329 LLPGGSVSMDGESPKTNTCIFSANEDMDTMRNFEQVFVKLMRRYKYLEKMF 481
           LLP GSVS     P   T  F   E +  +R+     ++     ++L++MF
Sbjct: 59  LLPSGSVSRLHRIPMAETWHFYLGEPLTVIRSLSTRSLQTSGLVRFLQRMF 109


>At3g13760.1 68416.m01736 DC1 domain-containing protein contains
           Pfam profile PF03107: DC1 domain
          Length = 652

 Score = 27.1 bits (57), Expect = 10.0
 Identities = 13/33 (39%), Positives = 16/33 (48%)
 Frame = +1

Query: 220 PVAISTQPTST*LGRIKTRLPTLWRSHIRCTHC 318
           PV IST  + T          T W+ HI+CT C
Sbjct: 473 PVVISTSYSDTDEILCNACQGTCWQPHIKCTKC 505


>At2g40230.1 68415.m04947 transferase family protein similar to
           taxadienol acetyl transferase from Taxus cuspidata
           [gi:6978038]; contains Pfam transferase family domain
           PF002458
          Length = 433

 Score = 27.1 bits (57), Expect = 10.0
 Identities = 18/57 (31%), Positives = 28/57 (49%), Gaps = 2/57 (3%)
 Frame = +3

Query: 417 CEISNRYS*N*CVGTST-WKKCFEEEMKKVSGYLKGFXPX-TAASKLGAHXWQLWIR 581
           C   NR++    V TS  +++    E+KK++  L  F    T+   L AH W+ W R
Sbjct: 219 CGFVNRFNAERLVPTSVVFERQKLNELKKLASRLGEFNSKHTSFEVLSAHVWRSWAR 275


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,146,725
Number of Sequences: 28952
Number of extensions: 312808
Number of successful extensions: 1011
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 931
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1010
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)