Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060272.seq
(686 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 165 4e-42
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 158 8e-40
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 143 2e-35
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 142 6e-35
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 108 8e-25
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 55 8e-09
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 44 2e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.039
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.83
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 5.9
SPBC1778.07 |||methyltransferase N2227 family|Schizosaccharomyce... 26 5.9
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 7.8
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 25 7.8
SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex al... 25 7.8
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 165 bits (402), Expect = 4e-42
Identities = 77/103 (74%), Positives = 88/103 (85%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
NP+NTIFDAKRLIGRKF+D VQ+DMKHWPF+V+S G P ++V YKGE KTF PEE+SS
Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119
Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
MVL KM+ETAEAYLG V +AV+ VPAYFNDSQRQATKDAG I
Sbjct: 120 MVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLI 162
Score = 107 bits (257), Expect = 1e-24
Identities = 49/58 (84%), Positives = 53/58 (91%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 158 bits (383), Expect = 8e-40
Identities = 73/103 (70%), Positives = 86/103 (83%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
NP+NTIFDAKRLIGR+F D VQ+DMKHWPF+V+ G P I+V +KGE KTF PEE+SS
Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119
Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
MVL KM+E+AEA+LG V +AV+ VPAYFNDSQRQATKDAG I
Sbjct: 120 MVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLI 162
Score = 107 bits (257), Expect = 1e-24
Identities = 49/58 (84%), Positives = 53/58 (91%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 143 bits (346), Expect = 2e-35
Identities = 66/103 (64%), Positives = 80/103 (77%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
NP NT+FDAKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+
Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISA 123
Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
MVLTKMKE +EA L K V+ AVI VPAYF+DSQR ATKDAG I
Sbjct: 124 MVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAI 166
Score = 95.5 bits (227), Expect = 6e-21
Identities = 43/58 (74%), Positives = 49/58 (84%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A P
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 142 bits (343), Expect = 6e-35
Identities = 65/103 (63%), Positives = 83/103 (80%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
NP NTIFD KRLIGRKF++ T+ D+K +PF +V+D P ++V G+ K F PEE+S+
Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152
Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
M+L+KMK+TAEAYLGK V +AV+ VPAYFND+QRQATKDAGTI
Sbjct: 153 MILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTI 195
Score = 99 bits (238), Expect = 3e-22
Identities = 68/180 (37%), Positives = 94/180 (52%), Gaps = 3/180 (1%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP-TT 268
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P T
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 269 QYSMPNVSSDVSSKMLLCKPT*STGLSRLSVMEDNLRSR*HIRV--KTKPFSPRKSVPWC 442
+ + + + + K S ++ D R + V K K F+P +
Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKS---FPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMI 154
Query: 443 LRK*RKLPKLISAKLCRMQLFTFPRTSMTLKDKPQKMQVPSSGLKTFLRIINXTPLAAAI 622
L K ++ + K + T P + + K +GL +RI+N P AAAI
Sbjct: 155 LSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNV-IRIVN-EPTAAAI 212
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 108 bits (259), Expect = 8e-25
Identities = 52/103 (50%), Positives = 68/103 (66%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
NP NT F KRLIGR+F++ VQ D+K P+++V + KT+ P ++
Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGG 165
Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
+L+KM+ETA YLGK V+NAV+ VPAYFNDSQRQATK AG I
Sbjct: 166 FILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAI 208
Score = 69.3 bits (162), Expect = 5e-13
Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%)
Frame = +2
Query: 80 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAR 256
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 257 TPTTQY 274
P +
Sbjct: 109 NPENTF 114
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 55.2 bits (127), Expect = 8e-09
Identities = 34/104 (32%), Positives = 53/104 (50%), Gaps = 1/104 (0%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQ-ADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVS 432
N NT+ KRL GR ++D ++ + ++ G KV Y E+ F ++
Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122
Query: 433 SMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
+ TK+K AEA L +V + VI +PA+F D QR+A +A I
Sbjct: 123 AAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANI 166
Score = 49.2 bits (112), Expect = 6e-07
Identities = 20/59 (33%), Positives = 38/59 (64%)
Frame = +2
Query: 77 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 44.4 bits (100), Expect = 2e-05
Identities = 29/106 (27%), Positives = 53/106 (50%), Gaps = 6/106 (5%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGED------KTFF 417
N +N++ + + L+G+ ++ T+ H+ V+ G K+ + ++ K
Sbjct: 82 NADNSVTNFRDLLGKSHDELTLHHC--HYSANPVNVEGQIGFKITVQEDEESDPKEKILT 139
Query: 418 PEEVSSMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDA 555
E S L ++ E+AE +LG V V+ VP YF D+QR+A + A
Sbjct: 140 AHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESA 185
Score = 41.5 bits (93), Expect = 1e-04
Identities = 17/55 (30%), Positives = 31/55 (56%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAR 256
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ R
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVR 81
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 33.1 bits (72), Expect = 0.039
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTPTT 268
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N R P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83
Query: 269 QYSMPNV 289
+S+ NV
Sbjct: 84 -HSIRNV 89
Score = 29.9 bits (64), Expect = 0.36
Identities = 14/55 (25%), Positives = 28/55 (50%)
Frame = +1
Query: 400 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
+++ + EE+ +M + AE + + + V+ VP +FN+ QR +A I
Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.83
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3
Query: 84 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 179
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.8 bits (54), Expect = 5.9
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = -1
Query: 170 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 12
G W SP S + H ++++ +F +RVL C+ L K +K+A L
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323
>SPBC1778.07 |||methyltransferase N2227 family|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 373
Score = 25.8 bits (54), Expect = 5.9
Identities = 10/43 (23%), Positives = 20/43 (46%)
Frame = -3
Query: 558 TCIFCGLSLRVIEVRGNVNNCILHSFAEISFGSFLHFRKHHGT 430
TC F + +++ + NC++ I+ G L+ + GT
Sbjct: 261 TCFFIDTTKNILDYLDTIKNCLVDGGYWINLGPLLYHFESEGT 303
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 200 AFTDTERLIGDAAKNQVARTPTTQYSMPNVSSDV 301
AF D + G AK A P+T+ + N+S V
Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188
>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 570
Score = 25.4 bits (53), Expect = 7.8
Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = -2
Query: 262 WGSRHLVLGGISDETLGVCERNIRRSGPVALVVGD-DLHLPVLEDTNARVR 113
WGS +G + TLG+ + + + L+VGD LHL V ++ +A +R
Sbjct: 418 WGSIGWSVGAMFGATLGIKDSDAPHRRSI-LIVGDGSLHLTV-QEISATIR 466
>SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex alpha
subunit Egd2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 173
Score = 25.4 bits (53), Expect = 7.8
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +1
Query: 427 VSSMVLTKMKETAEAYLGK-TVQNAVIHVPAYFNDSQRQATK 549
++ ++ K A LGK TV++ A FN+SQ+QAT+
Sbjct: 57 INEPIVYKSSNNAYIVLGKVTVEDMAAQARA-FNESQKQATE 97
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,121,352
Number of Sequences: 5004
Number of extensions: 68440
Number of successful extensions: 224
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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