SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060272.seq
         (686 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   165   4e-42
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   158   8e-40
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...   143   2e-35
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...   142   6e-35
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...   108   8e-25
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch...    55   8e-09
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom...    44   2e-05
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe...    33   0.039
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar...    29   0.83 
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar...    26   5.9  
SPBC1778.07 |||methyltransferase N2227 family|Schizosaccharomyce...    26   5.9  
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1...    25   7.8  
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe...    25   7.8  
SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex al...    25   7.8  

>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  165 bits (402), Expect = 4e-42
 Identities = 77/103 (74%), Positives = 88/103 (85%)
 Frame = +1

Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
           NP+NTIFDAKRLIGRKF+D  VQ+DMKHWPF+V+S  G P ++V YKGE KTF PEE+SS
Sbjct: 60  NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119

Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
           MVL KM+ETAEAYLG  V +AV+ VPAYFNDSQRQATKDAG I
Sbjct: 120 MVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLI 162



 Score =  107 bits (257), Expect = 1e-24
 Identities = 49/58 (84%), Positives = 53/58 (91%)
 Frame = +2

Query: 89  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA  P
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61


>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  158 bits (383), Expect = 8e-40
 Identities = 73/103 (70%), Positives = 86/103 (83%)
 Frame = +1

Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
           NP+NTIFDAKRLIGR+F D  VQ+DMKHWPF+V+   G P I+V +KGE KTF PEE+SS
Sbjct: 60  NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119

Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
           MVL KM+E+AEA+LG  V +AV+ VPAYFNDSQRQATKDAG I
Sbjct: 120 MVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLI 162



 Score =  107 bits (257), Expect = 1e-24
 Identities = 49/58 (84%), Positives = 53/58 (91%)
 Frame = +2

Query: 89  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
           ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA  P
Sbjct: 4   SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score =  143 bits (346), Expect = 2e-35
 Identities = 66/103 (64%), Positives = 80/103 (77%)
 Frame = +1

Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
           NP NT+FDAKRLIGR++ED   Q D+KHWPF+V+ + G P I+V Y GE K F  +E+S+
Sbjct: 64  NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISA 123

Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
           MVLTKMKE +EA L K V+ AVI VPAYF+DSQR ATKDAG I
Sbjct: 124 MVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAI 166



 Score = 95.5 bits (227), Expect = 6e-21
 Identities = 43/58 (74%), Positives = 49/58 (84%)
 Frame = +2

Query: 89  AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
           A+GIDLGTTYSCV V++   VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A  P
Sbjct: 8   AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score =  142 bits (343), Expect = 6e-35
 Identities = 65/103 (63%), Positives = 83/103 (80%)
 Frame = +1

Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
           NP NTIFD KRLIGRKF++ T+  D+K +PF +V+D   P ++V   G+ K F PEE+S+
Sbjct: 93  NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152

Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
           M+L+KMK+TAEAYLGK V +AV+ VPAYFND+QRQATKDAGTI
Sbjct: 153 MILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTI 195



 Score =   99 bits (238), Expect = 3e-22
 Identities = 68/180 (37%), Positives = 94/180 (52%), Gaps = 3/180 (1%)
 Frame = +2

Query: 92  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP-TT 268
           +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ    P  T
Sbjct: 38  IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97

Query: 269 QYSMPNVSSDVSSKMLLCKPT*STGLSRLSVMEDNLRSR*HIRV--KTKPFSPRKSVPWC 442
            + +  +      +  + K   S       ++ D  R    + V  K K F+P +     
Sbjct: 98  IFDIKRLIGRKFDEKTMAKDIKS---FPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMI 154

Query: 443 LRK*RKLPKLISAKLCRMQLFTFPRTSMTLKDKPQKMQVPSSGLKTFLRIINXTPLAAAI 622
           L K ++  +    K     + T P      + +  K     +GL   +RI+N  P AAAI
Sbjct: 155 LSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNV-IRIVN-EPTAAAI 212


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score =  108 bits (259), Expect = 8e-25
 Identities = 52/103 (50%), Positives = 68/103 (66%)
 Frame = +1

Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435
           NP NT F  KRLIGR+F++  VQ D+K  P+++V            +   KT+ P ++  
Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGG 165

Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
            +L+KM+ETA  YLGK V+NAV+ VPAYFNDSQRQATK AG I
Sbjct: 166 FILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAI 208



 Score = 69.3 bits (162), Expect = 5e-13
 Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%)
 Frame = +2

Query: 80  KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAR 256
           K P +GIDLGTT SC+ + +    ++IAN +G RTTPS VAFT D ERL+G +AK Q   
Sbjct: 49  KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108

Query: 257 TPTTQY 274
            P   +
Sbjct: 109 NPENTF 114


>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
           Pss1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 720

 Score = 55.2 bits (127), Expect = 8e-09
 Identities = 34/104 (32%), Positives = 53/104 (50%), Gaps = 1/104 (0%)
 Frame = +1

Query: 256 NPNNTIFDAKRLIGRKFEDATVQ-ADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVS 432
           N  NT+   KRL GR ++D  ++  +      ++    G    KV Y  E+  F   ++ 
Sbjct: 63  NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122

Query: 433 SMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
           +   TK+K  AEA L  +V + VI +PA+F D QR+A  +A  I
Sbjct: 123 AAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANI 166



 Score = 49.2 bits (112), Expect = 6e-07
 Identities = 20/59 (33%), Positives = 38/59 (64%)
 Frame = +2

Query: 77  AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253
           ++   VGID G + + + V ++  +++I N+  NR+TPS V++ +  R +G+AAK+  A
Sbjct: 3   SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61


>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 566

 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 29/106 (27%), Positives = 53/106 (50%), Gaps = 6/106 (5%)
 Frame = +1

Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGED------KTFF 417
           N +N++ + + L+G+  ++ T+     H+    V+  G    K+  + ++      K   
Sbjct: 82  NADNSVTNFRDLLGKSHDELTLHHC--HYSANPVNVEGQIGFKITVQEDEESDPKEKILT 139

Query: 418 PEEVSSMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDA 555
             E S   L ++ E+AE +LG  V   V+ VP YF D+QR+A + A
Sbjct: 140 AHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESA 185



 Score = 41.5 bits (93), Expect = 1e-04
 Identities = 17/55 (30%), Positives = 31/55 (56%)
 Frame = +2

Query: 92  VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAR 256
           +GI  G   S +   + GK +++AN++GNR  PS +++   +   G  A+ Q+ R
Sbjct: 27  IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVR 81


>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 848

 Score = 33.1 bits (72), Expect = 0.039
 Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
 Frame = +2

Query: 92  VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTPTT 268
           + ID GT ++   + + G  +EI+      R   S VAF   ER+ G  A N   R P  
Sbjct: 25  LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83

Query: 269 QYSMPNV 289
            +S+ NV
Sbjct: 84  -HSIRNV 89



 Score = 29.9 bits (64), Expect = 0.36
 Identities = 14/55 (25%), Positives = 28/55 (50%)
 Frame = +1

Query: 400 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564
           +++ +  EE+ +M +      AE    + + + V+ VP +FN+ QR    +A  I
Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185


>SPAC25B8.04c |||mitochondrial splicing suppressor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 378

 Score = 28.7 bits (61), Expect = 0.83
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = +3

Query: 84  HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 179
           HP   S W+P    LVS+    W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314


>SPAC823.11 |||sphingosine-1-phosphate phosphatase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 411

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 16/53 (30%), Positives = 26/53 (49%)
 Frame = -1

Query: 170 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 12
           G W  SP S +  H   ++++  +F +RVL   C+      L  K  +K+A L
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323


>SPBC1778.07 |||methyltransferase N2227 family|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 373

 Score = 25.8 bits (54), Expect = 5.9
 Identities = 10/43 (23%), Positives = 20/43 (46%)
 Frame = -3

Query: 558 TCIFCGLSLRVIEVRGNVNNCILHSFAEISFGSFLHFRKHHGT 430
           TC F   +  +++    + NC++     I+ G  L+  +  GT
Sbjct: 261 TCFFIDTTKNILDYLDTIKNCLVDGGYWINLGPLLYHFESEGT 303


>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 857

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 12/34 (35%), Positives = 17/34 (50%)
 Frame = +2

Query: 200 AFTDTERLIGDAAKNQVARTPTTQYSMPNVSSDV 301
           AF D  +  G  AK   A  P+T+  + N+S  V
Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188


>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 570

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
 Frame = -2

Query: 262 WGSRHLVLGGISDETLGVCERNIRRSGPVALVVGD-DLHLPVLEDTNARVR 113
           WGS    +G +   TLG+ + +      + L+VGD  LHL V ++ +A +R
Sbjct: 418 WGSIGWSVGAMFGATLGIKDSDAPHRRSI-LIVGDGSLHLTV-QEISATIR 466


>SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex alpha
           subunit Egd2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 173

 Score = 25.4 bits (53), Expect = 7.8
 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
 Frame = +1

Query: 427 VSSMVLTKMKETAEAYLGK-TVQNAVIHVPAYFNDSQRQATK 549
           ++  ++ K    A   LGK TV++      A FN+SQ+QAT+
Sbjct: 57  INEPIVYKSSNNAYIVLGKVTVEDMAAQARA-FNESQKQATE 97


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,121,352
Number of Sequences: 5004
Number of extensions: 68440
Number of successful extensions: 224
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 222
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -