BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060272.seq (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 165 4e-42 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 158 8e-40 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 143 2e-35 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 142 6e-35 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 108 8e-25 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 55 8e-09 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 44 2e-05 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.039 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.83 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 5.9 SPBC1778.07 |||methyltransferase N2227 family|Schizosaccharomyce... 26 5.9 SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 7.8 SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 25 7.8 SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex al... 25 7.8 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 165 bits (402), Expect = 4e-42 Identities = 77/103 (74%), Positives = 88/103 (85%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435 NP+NTIFDAKRLIGRKF+D VQ+DMKHWPF+V+S G P ++V YKGE KTF PEE+SS Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119 Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564 MVL KM+ETAEAYLG V +AV+ VPAYFNDSQRQATKDAG I Sbjct: 120 MVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKDAGLI 162 Score = 107 bits (257), Expect = 1e-24 Identities = 49/58 (84%), Positives = 53/58 (91%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 158 bits (383), Expect = 8e-40 Identities = 73/103 (70%), Positives = 86/103 (83%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435 NP+NTIFDAKRLIGR+F D VQ+DMKHWPF+V+ G P I+V +KGE KTF PEE+SS Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119 Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564 MVL KM+E+AEA+LG V +AV+ VPAYFNDSQRQATKDAG I Sbjct: 120 MVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKDAGLI 162 Score = 107 bits (257), Expect = 1e-24 Identities = 49/58 (84%), Positives = 53/58 (91%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 143 bits (346), Expect = 2e-35 Identities = 66/103 (64%), Positives = 80/103 (77%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435 NP NT+FDAKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+ Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISA 123 Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564 MVLTKMKE +EA L K V+ AVI VPAYF+DSQR ATKDAG I Sbjct: 124 MVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKDAGAI 166 Score = 95.5 bits (227), Expect = 6e-21 Identities = 43/58 (74%), Positives = 49/58 (84%) Frame = +2 Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A P Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 142 bits (343), Expect = 6e-35 Identities = 65/103 (63%), Positives = 83/103 (80%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435 NP NTIFD KRLIGRKF++ T+ D+K +PF +V+D P ++V G+ K F PEE+S+ Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152 Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564 M+L+KMK+TAEAYLGK V +AV+ VPAYFND+QRQATKDAGTI Sbjct: 153 MILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTI 195 Score = 99 bits (238), Expect = 3e-22 Identities = 68/180 (37%), Positives = 94/180 (52%), Gaps = 3/180 (1%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP-TT 268 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P T Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97 Query: 269 QYSMPNVSSDVSSKMLLCKPT*STGLSRLSVMEDNLRSR*HIRV--KTKPFSPRKSVPWC 442 + + + + + K S ++ D R + V K K F+P + Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKS---FPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMI 154 Query: 443 LRK*RKLPKLISAKLCRMQLFTFPRTSMTLKDKPQKMQVPSSGLKTFLRIINXTPLAAAI 622 L K ++ + K + T P + + K +GL +RI+N P AAAI Sbjct: 155 LSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNV-IRIVN-EPTAAAI 212 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 108 bits (259), Expect = 8e-25 Identities = 52/103 (50%), Positives = 68/103 (66%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVSS 435 NP NT F KRLIGR+F++ VQ D+K P+++V + KT+ P ++ Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGG 165 Query: 436 MVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564 +L+KM+ETA YLGK V+NAV+ VPAYFNDSQRQATK AG I Sbjct: 166 FILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAI 208 Score = 69.3 bits (162), Expect = 5e-13 Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%) Frame = +2 Query: 80 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAR 256 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 257 TPTTQY 274 P + Sbjct: 109 NPENTF 114 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 55.2 bits (127), Expect = 8e-09 Identities = 34/104 (32%), Positives = 53/104 (50%), Gaps = 1/104 (0%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQ-ADMKHWPFEVVSDGG*PKIKVAYKGEDKTFFPEEVS 432 N NT+ KRL GR ++D ++ + ++ G KV Y E+ F ++ Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122 Query: 433 SMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564 + TK+K AEA L +V + VI +PA+F D QR+A +A I Sbjct: 123 AAYFTKIKAIAEAELIGSVSDVVISIPAWFTDIQRRALLEAANI 166 Score = 49.2 bits (112), Expect = 6e-07 Identities = 20/59 (33%), Positives = 38/59 (64%) Frame = +2 Query: 77 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 44.4 bits (100), Expect = 2e-05 Identities = 29/106 (27%), Positives = 53/106 (50%), Gaps = 6/106 (5%) Frame = +1 Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGG*PKIKVAYKGED------KTFF 417 N +N++ + + L+G+ ++ T+ H+ V+ G K+ + ++ K Sbjct: 82 NADNSVTNFRDLLGKSHDELTLHHC--HYSANPVNVEGQIGFKITVQEDEESDPKEKILT 139 Query: 418 PEEVSSMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDA 555 E S L ++ E+AE +LG V V+ VP YF D+QR+A + A Sbjct: 140 AHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRKALESA 185 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/55 (30%), Positives = 31/55 (56%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAR 256 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ R Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVR 81 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 33.1 bits (72), Expect = 0.039 Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%) Frame = +2 Query: 92 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTPTT 268 + ID GT ++ + + G +EI+ R S VAF ER+ G A N R P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83 Query: 269 QYSMPNV 289 +S+ NV Sbjct: 84 -HSIRNV 89 Score = 29.9 bits (64), Expect = 0.36 Identities = 14/55 (25%), Positives = 28/55 (50%) Frame = +1 Query: 400 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVIHVPAYFNDSQRQATKDAGTI 564 +++ + EE+ +M + AE + + + V+ VP +FN+ QR +A I Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQRSILLEAARI 185 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.83 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +3 Query: 84 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 179 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.8 bits (54), Expect = 5.9 Identities = 16/53 (30%), Positives = 26/53 (49%) Frame = -1 Query: 170 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 12 G W SP S + H ++++ +F +RVL C+ L K +K+A L Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323 >SPBC1778.07 |||methyltransferase N2227 family|Schizosaccharomyces pombe|chr 2|||Manual Length = 373 Score = 25.8 bits (54), Expect = 5.9 Identities = 10/43 (23%), Positives = 20/43 (46%) Frame = -3 Query: 558 TCIFCGLSLRVIEVRGNVNNCILHSFAEISFGSFLHFRKHHGT 430 TC F + +++ + NC++ I+ G L+ + GT Sbjct: 261 TCFFIDTTKNILDYLDTIKNCLVDGGYWINLGPLLYHFESEGT 303 >SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 857 Score = 25.4 bits (53), Expect = 7.8 Identities = 12/34 (35%), Positives = 17/34 (50%) Frame = +2 Query: 200 AFTDTERLIGDAAKNQVARTPTTQYSMPNVSSDV 301 AF D + G AK A P+T+ + N+S V Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188 >SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 570 Score = 25.4 bits (53), Expect = 7.8 Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = -2 Query: 262 WGSRHLVLGGISDETLGVCERNIRRSGPVALVVGD-DLHLPVLEDTNARVR 113 WGS +G + TLG+ + + + L+VGD LHL V ++ +A +R Sbjct: 418 WGSIGWSVGAMFGATLGIKDSDAPHRRSI-LIVGDGSLHLTV-QEISATIR 466 >SPBC25H2.05 |egd2|nac1|nascent polypeptide-associated complex alpha subunit Egd2|Schizosaccharomyces pombe|chr 2|||Manual Length = 173 Score = 25.4 bits (53), Expect = 7.8 Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%) Frame = +1 Query: 427 VSSMVLTKMKETAEAYLGK-TVQNAVIHVPAYFNDSQRQATK 549 ++ ++ K A LGK TV++ A FN+SQ+QAT+ Sbjct: 57 INEPIVYKSSNNAYIVLGKVTVEDMAAQARA-FNESQKQATE 97 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,121,352 Number of Sequences: 5004 Number of extensions: 68440 Number of successful extensions: 224 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 207 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 222 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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