Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060267.seq
(565 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g02100.3 68414.m00136 leucine carboxyl methyltransferase fami... 56 2e-08
At1g02100.2 68414.m00134 leucine carboxyl methyltransferase fami... 56 2e-08
At1g02100.1 68414.m00135 leucine carboxyl methyltransferase fami... 56 2e-08
At4g34460.3 68417.m04900 guanine nucleotide-binding protein beta... 30 0.93
At4g34460.2 68417.m04898 guanine nucleotide-binding protein beta... 30 0.93
At4g34460.1 68417.m04899 guanine nucleotide-binding protein beta... 30 0.93
At3g58340.1 68416.m06503 meprin and TRAF homology domain-contain... 28 3.7
At5g05150.1 68418.m00547 transport protein-related contains 2 WD... 28 5.0
At1g60500.1 68414.m06811 dynamin family protein similar to RBTMx... 28 5.0
At3g01780.1 68416.m00118 expressed protein est hit, 27 6.5
>At1g02100.3 68414.m00136 leucine carboxyl methyltransferase family
protein contains Pfam PF04072: Leucine carboxyl
methyltransferase domain; similar to Leucine carboxyl
methyltransferase (Protein-leucine O-
methyltransferase) (CGI-68) (SP:Q9UIC8) {Homo sapiens}
Length = 332
Score = 56.0 bits (129), Expect = 2e-08
Identities = 34/99 (34%), Positives = 53/99 (53%), Gaps = 1/99 (1%)
Frame = +3
Query: 243 GILRRVKGVEMFIHQFL-ERCDTKCQIINLGCGFDTLYWRLKDTTQAVGNFIELDFPAVT 419
G R + QFL +K QI++LG GFDT Y++L D ++ELDF VT
Sbjct: 53 GYFSRWAAFRKLMSQFLLSGTSSKKQILSLGAGFDTTYFQLLDEGNGPNLYVELDFKEVT 112
Query: 420 AKKAHIIKRNXALLEKICNEDG*VVIREXRLHSDRXHLV 536
+KKA +I+ + L +K+ + + I E ++ SD L+
Sbjct: 113 SKKAAVIQNSSQLRDKL-GANASISIDEGQVLSDHYKLL 150
Score = 40.7 bits (91), Expect = 7e-04
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +1
Query: 151 KRCAVELGYWKDDYIGYFAKHVDRKAPEINRGYYA 255
K V+ GY KDDY+ F K R++P INRGY++
Sbjct: 22 KLSCVKKGYMKDDYVHLFVKRPVRRSPIINRGYFS 56
>At1g02100.2 68414.m00134 leucine carboxyl methyltransferase family
protein contains Pfam PF04072: Leucine carboxyl
methyltransferase domain; similar to Leucine carboxyl
methyltransferase (Protein-leucine O-
methyltransferase) (CGI-68) (SP:Q9UIC8) {Homo sapiens}
Length = 306
Score = 56.0 bits (129), Expect = 2e-08
Identities = 34/99 (34%), Positives = 53/99 (53%), Gaps = 1/99 (1%)
Frame = +3
Query: 243 GILRRVKGVEMFIHQFL-ERCDTKCQIINLGCGFDTLYWRLKDTTQAVGNFIELDFPAVT 419
G R + QFL +K QI++LG GFDT Y++L D ++ELDF VT
Sbjct: 53 GYFSRWAAFRKLMSQFLLSGTSSKKQILSLGAGFDTTYFQLLDEGNGPNLYVELDFKEVT 112
Query: 420 AKKAHIIKRNXALLEKICNEDG*VVIREXRLHSDRXHLV 536
+KKA +I+ + L +K+ + + I E ++ SD L+
Sbjct: 113 SKKAAVIQNSSQLRDKL-GANASISIDEGQVLSDHYKLL 150
Score = 40.7 bits (91), Expect = 7e-04
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +1
Query: 151 KRCAVELGYWKDDYIGYFAKHVDRKAPEINRGYYA 255
K V+ GY KDDY+ F K R++P INRGY++
Sbjct: 22 KLSCVKKGYMKDDYVHLFVKRPVRRSPIINRGYFS 56
>At1g02100.1 68414.m00135 leucine carboxyl methyltransferase family
protein contains Pfam PF04072: Leucine carboxyl
methyltransferase domain; similar to Leucine carboxyl
methyltransferase (Protein-leucine O-
methyltransferase) (CGI-68) (SP:Q9UIC8) {Homo sapiens}
Length = 332
Score = 56.0 bits (129), Expect = 2e-08
Identities = 34/99 (34%), Positives = 53/99 (53%), Gaps = 1/99 (1%)
Frame = +3
Query: 243 GILRRVKGVEMFIHQFL-ERCDTKCQIINLGCGFDTLYWRLKDTTQAVGNFIELDFPAVT 419
G R + QFL +K QI++LG GFDT Y++L D ++ELDF VT
Sbjct: 53 GYFSRWAAFRKLMSQFLLSGTSSKKQILSLGAGFDTTYFQLLDEGNGPNLYVELDFKEVT 112
Query: 420 AKKAHIIKRNXALLEKICNEDG*VVIREXRLHSDRXHLV 536
+KKA +I+ + L +K+ + + I E ++ SD L+
Sbjct: 113 SKKAAVIQNSSQLRDKL-GANASISIDEGQVLSDHYKLL 150
Score = 40.7 bits (91), Expect = 7e-04
Identities = 17/35 (48%), Positives = 23/35 (65%)
Frame = +1
Query: 151 KRCAVELGYWKDDYIGYFAKHVDRKAPEINRGYYA 255
K V+ GY KDDY+ F K R++P INRGY++
Sbjct: 22 KLSCVKKGYMKDDYVHLFVKRPVRRSPIINRGYFS 56
>At4g34460.3 68417.m04900 guanine nucleotide-binding protein beta
subunit (GB1) / GTP-binding protein beta subunit (AGB1)
/ transducin contains 7 WD-40 repeats (PF00400);
identical to Guanine nucleotide-binding protein beta
subunit.SP:P49177 [Arabidopsis thaliana]; Weiss, CA et
al, PNAS 91:9954 (1994)
Length = 347
Score = 30.3 bits (65), Expect = 0.93
Identities = 16/40 (40%), Positives = 28/40 (70%)
Frame = -1
Query: 484 PSSLQIFSSSAXLRLMI*AFFAVTAGKSNSIKLPTAWVVS 365
P +I S+S RL++ + A+T+ K+++IKLP AWV++
Sbjct: 75 PERNRIVSASQDGRLIV--WNALTSQKTHAIKLPCAWVMT 112
>At4g34460.2 68417.m04898 guanine nucleotide-binding protein beta
subunit (GB1) / GTP-binding protein beta subunit (AGB1)
/ transducin contains 7 WD-40 repeats (PF00400);
identical to Guanine nucleotide-binding protein beta
subunit.SP:P49177 [Arabidopsis thaliana]; Weiss, CA et
al, PNAS 91:9954 (1994)
Length = 315
Score = 30.3 bits (65), Expect = 0.93
Identities = 16/40 (40%), Positives = 28/40 (70%)
Frame = -1
Query: 484 PSSLQIFSSSAXLRLMI*AFFAVTAGKSNSIKLPTAWVVS 365
P +I S+S RL++ + A+T+ K+++IKLP AWV++
Sbjct: 13 PERNRIVSASQDGRLIV--WNALTSQKTHAIKLPCAWVMT 50
>At4g34460.1 68417.m04899 guanine nucleotide-binding protein beta
subunit (GB1) / GTP-binding protein beta subunit (AGB1)
/ transducin contains 7 WD-40 repeats (PF00400);
identical to Guanine nucleotide-binding protein beta
subunit.SP:P49177 [Arabidopsis thaliana]; Weiss, CA et
al, PNAS 91:9954 (1994)
Length = 377
Score = 30.3 bits (65), Expect = 0.93
Identities = 16/40 (40%), Positives = 28/40 (70%)
Frame = -1
Query: 484 PSSLQIFSSSAXLRLMI*AFFAVTAGKSNSIKLPTAWVVS 365
P +I S+S RL++ + A+T+ K+++IKLP AWV++
Sbjct: 75 PERNRIVSASQDGRLIV--WNALTSQKTHAIKLPCAWVMT 112
>At3g58340.1 68416.m06503 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 325
Score = 28.3 bits (60), Expect = 3.7
Identities = 10/38 (26%), Positives = 27/38 (71%), Gaps = 1/38 (2%)
Frame = +3
Query: 222 KSTRDQSGILRRVKGVEMFIHQFLERCD-TKCQIINLG 332
K ++QSG++ ++G+E +H+ + +C+ K +++++G
Sbjct: 280 KKEKEQSGLII-LQGIEQQLHELMHKCEKKKSEVLSVG 316
>At5g05150.1 68418.m00547 transport protein-related contains 2 WD-40
repeats (PF00400); similar to transport protein Gsa12p
(GI:18307769) [Pichia pastoris]
Length = 374
Score = 27.9 bits (59), Expect = 5.0
Identities = 21/51 (41%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = -3
Query: 455 CXVALDDMSLLCSNSWKIQLYKIANCL-GRVLQSPVKSVESTAKIYNLAFS 306
C D SLL + S K L +I N + G +LQ + VE A+IYN+A S
Sbjct: 209 CMTLTLDGSLLATASTKGTLIRIFNAVDGTLLQEFRRGVER-AEIYNVAIS 258
>At1g60500.1 68414.m06811 dynamin family protein similar to RBTMx2
[Oncorhynchus mykiss] GI:1399452; contains Pfam profiles
PF01031: Dynamin central region, PF00350: Dynamin
family, PF02212: Dynamin GTPase effector domain
Length = 669
Score = 27.9 bits (59), Expect = 5.0
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = -3
Query: 437 DMSLLCSNSWKIQLYKIANCLGRVLQSPVKSVEST 333
DM + ++ WKI L +I + L LQ VKS+ +T
Sbjct: 577 DMKMRITSYWKIVLRRIVDNLALYLQLSVKSLVNT 611
>At3g01780.1 68416.m00118 expressed protein est hit,
Length = 1176
Score = 27.5 bits (58), Expect = 6.5
Identities = 13/44 (29%), Positives = 26/44 (59%)
Frame = -3
Query: 455 CXVALDDMSLLCSNSWKIQLYKIANCLGRVLQSPVKSVESTAKI 324
C +A DD+ LC N+ + L K++N R+ Q+ + ++ +K+
Sbjct: 141 CILARDDLVTLCENNVGL-LDKVSNWWARIGQNMLDKSDAVSKV 183
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,686,115
Number of Sequences: 28952
Number of extensions: 228178
Number of successful extensions: 491
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 478
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 491
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1082538160
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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