BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060259.seq (685 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 29 0.83 SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 29 0.83 SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 1.9 SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 27 1.9 SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 2.5 SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosacchar... 27 3.3 SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizos... 26 4.4 SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pom... 26 5.8 >SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1063 Score = 28.7 bits (61), Expect = 0.83 Identities = 17/60 (28%), Positives = 25/60 (41%), Gaps = 1/60 (1%) Frame = +1 Query: 292 CGNSLGYPLCNCVHPEEDHAVDWSAVCTAFCTDGYTLGGCPAC-ASQRSSPVAIAGRILN 468 CG+S G LCN + D + + C D GCP C S + +G +L+ Sbjct: 965 CGDSSGAGLCNKAYEHTDELITMAIERIESC-DCKVREGCPGCITSSKFEGGVCSGEVLD 1023 >SPBP8B7.23 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 673 Score = 28.7 bits (61), Expect = 0.83 Identities = 12/37 (32%), Positives = 17/37 (45%) Frame = +1 Query: 337 EEDHAVDWSAVCTAFCTDGYTLGGCPACASQRSSPVA 447 + D V W V C+ + L CP C ++ PVA Sbjct: 190 DPDSPVKWEDVWQVLCSSDFQLAACPFCLEEK--PVA 224 >SPBC1685.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 325 Score = 27.5 bits (58), Expect = 1.9 Identities = 15/48 (31%), Positives = 25/48 (52%) Frame = -3 Query: 497 LHQACQPSEVLRIRPAMATGLLRWEAQAGQPPSVYPSVQNAVQTALQS 354 L Q+ QP+++ RIR A+A L W + +Y S Q +++ S Sbjct: 67 LFQSKQPNDLTRIRKAIAEQLQLWCQACQENADLYRSQQRKLKSRWDS 114 >SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase |Schizosaccharomyces pombe|chr 2|||Manual Length = 378 Score = 27.5 bits (58), Expect = 1.9 Identities = 13/19 (68%), Positives = 13/19 (68%) Frame = +1 Query: 352 VDWSAVCTAFCTDGYTLGG 408 VDWSAVC TD YTL G Sbjct: 41 VDWSAVCH---TDAYTLSG 56 >SPAC29B12.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 682 Score = 27.1 bits (57), Expect = 2.5 Identities = 13/45 (28%), Positives = 21/45 (46%) Frame = -3 Query: 413 GQPPSVYPSVQNAVQTALQSTAWSSSGCTQLHNG*PKLLPQSAGH 279 GQP ++YP + + + ST ++SS + H LP H Sbjct: 256 GQPQALYPFIYQSRNVPMGSTMFASSNQSAAHPDGNNALPMDNTH 300 >SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 26.6 bits (56), Expect = 3.3 Identities = 7/16 (43%), Positives = 9/16 (56%) Frame = +1 Query: 142 VVCTFLWSWRGGLWRD 189 V C ++W W G W D Sbjct: 411 VTCGYMWYWNNGTWDD 426 >SPBC211.02c |cwf3|syf1|complexed with Cdc5 protein Cwf3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 790 Score = 26.2 bits (55), Expect = 4.4 Identities = 11/26 (42%), Positives = 15/26 (57%) Frame = +1 Query: 43 NYSNQRDKRFYFYLSFSLYEHKTELF 120 NY+N ++ YF SF +YE LF Sbjct: 519 NYANLLEENAYFEDSFKIYERGVALF 544 >SPAP8A3.11c |||mitochondrial GTPase Mtg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 25.8 bits (54), Expect = 5.8 Identities = 9/15 (60%), Positives = 12/15 (80%) Frame = -2 Query: 198 WVQVPPQTAPPRPKK 154 WVQ+P +T PP+ KK Sbjct: 137 WVQMPGKTKPPKLKK 151 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,816,300 Number of Sequences: 5004 Number of extensions: 60759 Number of successful extensions: 188 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 178 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 188 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 315915086 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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