Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060259.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40480.1 68418.m04909 expressed protein ; expression supporte... 30 1.6
At5g67470.1 68418.m08507 formin homology 2 domain-containing pro... 29 3.8
At4g38240.2 68417.m05401 alpha-1,3-mannosyl-glycoprotein beta-1,... 29 3.8
At4g38240.1 68417.m05400 alpha-1,3-mannosyl-glycoprotein beta-1,... 29 3.8
At1g68570.1 68414.m07834 proton-dependent oligopeptide transport... 29 3.8
At4g36020.1 68417.m05128 cold-shock DNA-binding family protein c... 28 5.0
At5g39290.1 68418.m04758 expansin, putative (EXP26) similar to a... 27 8.8
At5g39270.1 68418.m04756 expansin, putative (EXP22) similar to e... 27 8.8
>At5g40480.1 68418.m04909 expressed protein ; expression supported by
MPSS
Length = 1919
Score = 29.9 bits (64), Expect = 1.6
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = +2
Query: 431 GAALWPSPVGSSTLLRVGRPGATFQCARGMAGPR--ATAIRTPFPV 562
G+ LW + V +S + V RP + QC++ M P+ T I T + +
Sbjct: 973 GSCLWEAVVNNSRVAEVIRPPSGLQCSQMMLSPKGLGTTIVTVYDI 1018
>At5g67470.1 68418.m08507 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 899
Score = 28.7 bits (61), Expect = 3.8
Identities = 22/88 (25%), Positives = 37/88 (42%)
Frame = +2
Query: 311 IHYVTACTLRKTTPWTGAPSAPHSVLMDIRWEVAQPVPPNGAALWPSPVGSSTLLRVGRP 490
++ V+A +L K+ PS P L + W+ + + A +W SS L R
Sbjct: 439 VNSVSAGSLEKSGDGDTDPSKPK--LKPLHWDKVR-ASSDRATVWDQLKSSSFQLNEDRM 495
Query: 491 GATFQCARGMAGPRATAIRTPFPVNKYE 574
F C G + P+ R+ P+ + E
Sbjct: 496 EHLFGCNSGSSAPKEPVRRSVIPLAENE 523
>At4g38240.2 68417.m05401 alpha-1,3-mannosyl-glycoprotein
beta-1,2-N-acetylglucosaminyltransferase, putative
similar to N-acetylglucosaminyltransferase I from
Arabidopsis thaliana [gi:5139335]; contains AT-AC
non-consensus splice sites at intron 13
Length = 444
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/40 (40%), Positives = 18/40 (45%), Gaps = 1/40 (2%)
Frame = -1
Query: 214 TYGDHLGASPA-TNRPAKTKEKYKQHGHYIFLRTILFYVH 98
TY HL P T RP + YK HY + LFY H
Sbjct: 161 TYMQHLDFEPVVTERPGELTAYYKIARHYKWALDQLFYKH 200
>At4g38240.1 68417.m05400 alpha-1,3-mannosyl-glycoprotein
beta-1,2-N-acetylglucosaminyltransferase, putative
similar to N-acetylglucosaminyltransferase I from
Arabidopsis thaliana [gi:5139335]; contains AT-AC
non-consensus splice sites at intron 13
Length = 444
Score = 28.7 bits (61), Expect = 3.8
Identities = 16/40 (40%), Positives = 18/40 (45%), Gaps = 1/40 (2%)
Frame = -1
Query: 214 TYGDHLGASPA-TNRPAKTKEKYKQHGHYIFLRTILFYVH 98
TY HL P T RP + YK HY + LFY H
Sbjct: 161 TYMQHLDFEPVVTERPGELTAYYKIARHYKWALDQLFYKH 200
>At1g68570.1 68414.m07834 proton-dependent oligopeptide transport
(POT) family protein contains Pfam profile: PF00854 POT
family
Length = 596
Score = 28.7 bits (61), Expect = 3.8
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = -3
Query: 521 PCPWRTGTLHQACQPSEVLRIRPAMATGLLRWEAQAGQ 408
P WR T+H+ + V+R+ P A+G+L A A Q
Sbjct: 314 PNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQ 351
>At4g36020.1 68417.m05128 cold-shock DNA-binding family protein
contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
domain and PF00098: Zinc knuckle
Length = 299
Score = 28.3 bits (60), Expect = 5.0
Identities = 22/70 (31%), Positives = 32/70 (45%), Gaps = 2/70 (2%)
Frame = -2
Query: 567 LFTGNGVRIAVARGPAMPLAHWNV-APGLPTLRSVEDPTGDGHRAAPLGGTGWATSQRIS 391
L G+ V A+ +G NV APG +L+ + G+G R GG+G +
Sbjct: 51 LTVGDAVEFAITQGSDGKTKAVNVTAPGGGSLKKENNSRGNGARRGG-GGSGCYNCGELG 109
Query: 390 -ISTECGADG 364
IS +CG G
Sbjct: 110 HISKDCGIGG 119
>At5g39290.1 68418.m04758 expansin, putative (EXP26) similar to
alpha-expansin 4 precursor GI:16923355 from [Cucumis
sativus]; alpha-expansin gene family, PMID:11641069
Length = 263
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/57 (29%), Positives = 22/57 (38%), Gaps = 9/57 (15%)
Frame = +1
Query: 361 SAVCTAFCTDGYTLGGCPACASQRSSPVAIAGRI---------LNTSEGWQAWCNVP 504
+A+ TA DGYT G C R + G + N S+ WCN P
Sbjct: 78 AALSTALFNDGYTCGACYEIMCTRDPQWCLPGSVKITATNFCPANYSKTTDLWCNPP 134
>At5g39270.1 68418.m04756 expansin, putative (EXP22) similar to
expansin - Prunus armeniaca, EMBL:U93167; alpha-expansin
gene family, PMID:11641069
Length = 261
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/57 (29%), Positives = 22/57 (38%), Gaps = 9/57 (15%)
Frame = +1
Query: 361 SAVCTAFCTDGYTLGGCPACASQRSSPVAIAGRI---------LNTSEGWQAWCNVP 504
+A+ TA DGYT G C R + G + N S+ WCN P
Sbjct: 76 AALSTALFNDGYTCGACYEIMCTRDPQWCLPGSVKITATNFCPANYSKTTDLWCNPP 132
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,355,149
Number of Sequences: 28952
Number of extensions: 358521
Number of successful extensions: 1085
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1033
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1085
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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