Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060257.seq
(688 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_O14744 Cluster: Protein arginine N-methyltransferase 5;... 75 2e-12
UniRef50_A7S3Y9 Cluster: Predicted protein; n=7; Eumetazoa|Rep: ... 57 4e-07
UniRef50_UPI00015B5DBC Cluster: PREDICTED: similar to protein ar... 56 6e-07
UniRef50_UPI0000E4A113 Cluster: PREDICTED: hypothetical protein,... 56 1e-06
UniRef50_UPI0000D55AB2 Cluster: PREDICTED: similar to SKB1 homol... 50 4e-05
UniRef50_P46580 Cluster: Putative protein tag-251; n=3; cellular... 47 4e-04
UniRef50_Q54KI3 Cluster: Putative uncharacterized protein; n=2; ... 45 0.002
UniRef50_A7P546 Cluster: Chromosome chr4 scaffold_6, whole genom... 41 0.033
UniRef50_Q9U6Y9 Cluster: Protein arginine N-methyltransferase ca... 40 0.043
UniRef50_Q9VZM5 Cluster: GPI mannosyltransferase 3; n=5; Diptera... 35 2.1
UniRef50_A6S0C6 Cluster: Putative uncharacterized protein; n=1; ... 34 2.8
UniRef50_A3I251 Cluster: Putative uncharacterized protein; n=1; ... 34 3.7
UniRef50_A7EAP0 Cluster: Putative uncharacterized protein; n=2; ... 34 3.7
UniRef50_Q1F0S7 Cluster: Beta-xylosidase-like; n=1; Clostridium ... 33 4.9
UniRef50_Q5CVH1 Cluster: SANT domain containing protein; n=2; Cr... 33 8.6
>UniRef50_O14744 Cluster: Protein arginine N-methyltransferase 5;
n=33; Euteleostomi|Rep: Protein arginine
N-methyltransferase 5 - Homo sapiens (Human)
Length = 637
Score = 74.9 bits (176), Expect = 2e-12
Identities = 32/90 (35%), Positives = 55/90 (61%)
Frame = +1
Query: 259 QDWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMISIHGRESNNLA 438
+DW + IV KLSP+I DS +R+ E + +EL++ LG+PA ++ ++ ++ NLA
Sbjct: 76 RDWNTLIVGKLSPWIRPDSKVEKIRRNSEAAMLQELNFGAYLGLPAFLLPLNQEDNTNLA 135
Query: 439 RILQTYYETSHHPSLIWACVPMLLVEHIEN 528
R+L + T HH S+ W VP++ E + +
Sbjct: 136 RVLTNHIHTGHHSSMFWMRVPLVAPEDLRD 165
Score = 32.7 bits (71), Expect = 8.6
Identities = 17/60 (28%), Positives = 29/60 (48%), Gaps = 3/60 (5%)
Frame = +2
Query: 83 ISCGYEYIITADLQTCLTEALQCSYSFIVSPIIHPRFRR---QSTNAGKNGGFTRSDMVL 253
+S G + ++ L + + F+ P+ HPRF+R Q + G TRSD++L
Sbjct: 14 VSSGRDLNCVPEIADTLGAVAKQGFDFLCMPVFHPRFKREFIQEPAKNRPGPQTRSDLLL 73
>UniRef50_A7S3Y9 Cluster: Predicted protein; n=7; Eumetazoa|Rep:
Predicted protein - Nematostella vectensis
Length = 575
Score = 57.2 bits (132), Expect = 4e-07
Identities = 28/65 (43%), Positives = 41/65 (63%)
Frame = +1
Query: 259 QDWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMISIHGRESNNLA 438
QDW+S IV K+SP+INV S + VR+ E L +E++Y LG+P++M+ + NLA
Sbjct: 68 QDWSSLIVGKISPWINVGSLNEVVRKNSEKALMQEVNYAIHLGLPSVMLELGNYNIINLA 127
Query: 439 RILQT 453
L T
Sbjct: 128 HYLIT 132
Score = 45.2 bits (102), Expect = 0.002
Identities = 22/59 (37%), Positives = 33/59 (55%), Gaps = 2/59 (3%)
Frame = +2
Query: 83 ISCGYEYIITADLQTCLTEALQCSYSFIVSPIIHPRFRRQSTN--AGKNGGFTRSDMVL 253
+SCG + DL L A Q + FI +PI HPR++R+ ++ FTR+D+VL
Sbjct: 7 LSCGRDLTSIPDLVVALGSASQSGFDFICAPICHPRYKREFLEEIPDRSKSFTRADLVL 65
>UniRef50_UPI00015B5DBC Cluster: PREDICTED: similar to protein
arginine N-methyltransferase 5 (predicted); n=1; Nasonia
vitripennis|Rep: PREDICTED: similar to protein arginine
N-methyltransferase 5 (predicted) - Nasonia vitripennis
Length = 628
Score = 56.4 bits (130), Expect = 6e-07
Identities = 33/84 (39%), Positives = 48/84 (57%), Gaps = 3/84 (3%)
Frame = +1
Query: 262 DWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMISIHGRESN--NL 435
DW++ +V KLSP+I VDS +V + E+ L +EL+ LG+ AI I + G N NL
Sbjct: 69 DWSTLVVGKLSPHIYVDSKIHSVAKNSEETLLQELALASHLGLVAITIKLKGNIENNMNL 128
Query: 436 ARIL-QTYYETSHHPSLIWACVPM 504
ARI+ T + + +W VPM
Sbjct: 129 ARIMFDKLSTTQNFQAQVWIQVPM 152
Score = 46.8 bits (106), Expect = 5e-04
Identities = 20/61 (32%), Positives = 36/61 (59%), Gaps = 2/61 (3%)
Frame = +2
Query: 77 QEISCGYEYIITADLQTCLTEALQCSYSFIVSPIIHPRFRRQ--STNAGKNGGFTRSDMV 250
+ +SCG ++ DL+ CL A Y F+ P++HP ++R+ S ++ +TRSD++
Sbjct: 5 KNVSCGLDFCSVPDLKDCLYVANCSKYHFVCIPLVHPNYKREFISPEIKRSEPWTRSDLI 64
Query: 251 L 253
L
Sbjct: 65 L 65
>UniRef50_UPI0000E4A113 Cluster: PREDICTED: hypothetical protein,
partial; n=2; Strongylocentrotus purpuratus|Rep:
PREDICTED: hypothetical protein, partial -
Strongylocentrotus purpuratus
Length = 146
Score = 55.6 bits (128), Expect = 1e-06
Identities = 24/68 (35%), Positives = 43/68 (63%)
Frame = +1
Query: 259 QDWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMISIHGRESNNLA 438
QDW++ +V KLS ++ VD+ + VRQ + L +EL+Y L +PA+++ ++ NLA
Sbjct: 39 QDWSALVVGKLSEWLQVDAENTVVRQNSQVALMQELNYAAHLSLPAVLVPLNNINCVNLA 98
Query: 439 RILQTYYE 462
R L ++ +
Sbjct: 99 RCLYSHMQ 106
>UniRef50_UPI0000D55AB2 Cluster: PREDICTED: similar to SKB1 homolog;
n=1; Tribolium castaneum|Rep: PREDICTED: similar to SKB1
homolog - Tribolium castaneum
Length = 624
Score = 50.4 bits (115), Expect = 4e-05
Identities = 30/101 (29%), Positives = 47/101 (46%)
Frame = +1
Query: 262 DWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMISIHGRESNNLAR 441
+W IVA+L+P INVDS V+++ + +EL + LGVP I S+ R + L R
Sbjct: 79 EWGRYIVAELTPTINVDSEIEHVQRKSKALFLQELGFAVHLGVPVIKFSLTKRHNAQLGR 138
Query: 442 ILQTYYETSHHPSLIWACVPMLLVEHIENALKMMKKRKLWE 564
++ + S W +PM+ +K WE
Sbjct: 139 LINEKL-VNGFTSSFWVTLPMVHPSQFSPICTEDEKEDSWE 178
>UniRef50_P46580 Cluster: Putative protein tag-251; n=3; cellular
organisms|Rep: Putative protein tag-251 - Caenorhabditis
elegans
Length = 734
Score = 47.2 bits (107), Expect = 4e-04
Identities = 25/79 (31%), Positives = 40/79 (50%)
Frame = +1
Query: 265 WTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMISIHGRESNNLARI 444
W S +V K+SP+I+ DS E++L +ELSY LG+ + I + S A I
Sbjct: 119 WESYVVGKISPWIDCDSSDPAFASLSEEHLLKELSYICYLGLQTMAIELTRISSPRTAAI 178
Query: 445 LQTYYETSHHPSLIWACVP 501
L+ + T + +W +P
Sbjct: 179 LKKWIWTRNSRFTVWVQLP 197
>UniRef50_Q54KI3 Cluster: Putative uncharacterized protein; n=2;
Eukaryota|Rep: Putative uncharacterized protein -
Dictyostelium discoideum AX4
Length = 642
Score = 44.8 bits (101), Expect = 0.002
Identities = 27/67 (40%), Positives = 34/67 (50%), Gaps = 6/67 (8%)
Frame = +2
Query: 71 AQQEISCGYEYIITADLQTCLTEALQCSYSFIVSPIIHPRFRRQSTNA------GKNGGF 232
AQ E SCG E + + D+Q + A Y FI++ I HPRF R T A F
Sbjct: 5 AQYEFSCGVE-LESVDIQLDIERAYDLEYQFIMTSISHPRFNRDFTKASIGNSFSNKVAF 63
Query: 233 TRSDMVL 253
TRSD +L
Sbjct: 64 TRSDTLL 70
Score = 40.3 bits (90), Expect = 0.043
Identities = 22/82 (26%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = +1
Query: 265 WTSRIVAKLSPY-INVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMISIHGRESNNLAR 441
W S IV K S I++DS T+R L +E+S+ L +P+I++ S N A+
Sbjct: 75 WRSSIVGKTSTNGIDLDSIDPTIRSNSVKTLKQEISWAAHLSLPSILLPTPSFNSTNYAQ 134
Query: 442 ILQTYYETSHHPSLIWACVPML 507
++ ++ + +W +P++
Sbjct: 135 VVNQSLQSLSYMK-VWIRIPLV 155
>UniRef50_A7P546 Cluster: Chromosome chr4 scaffold_6, whole genome
shotgun sequence; n=1; Vitis vinifera|Rep: Chromosome
chr4 scaffold_6, whole genome shotgun sequence - Vitis
vinifera (Grape)
Length = 657
Score = 40.7 bits (91), Expect = 0.033
Identities = 23/84 (27%), Positives = 43/84 (51%), Gaps = 1/84 (1%)
Frame = +1
Query: 256 PQDWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMI-SIHGRESNN 432
P W+S +V KLS +I++DS +R E L +E+++ L + A ++ + G N
Sbjct: 78 PAQWSSHVVGKLSSWIDLDSEDKILRLDSEITLKQEIAWASHLSLQACLLPTPRGASCAN 137
Query: 433 LARILQTYYETSHHPSLIWACVPM 504
AR + + ++ L W +P+
Sbjct: 138 YARCVNQILQGLNNMQL-WLRIPL 160
>UniRef50_Q9U6Y9 Cluster: Protein arginine N-methyltransferase
capsuleen; n=3; Sophophora|Rep: Protein arginine
N-methyltransferase capsuleen - Drosophila melanogaster
(Fruit fly)
Length = 610
Score = 40.3 bits (90), Expect = 0.043
Identities = 19/63 (30%), Positives = 38/63 (60%), Gaps = 1/63 (1%)
Frame = +1
Query: 262 DWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGL-GVPAIMISIHGRESNNLA 438
DW S+++ +S +NVDSP+ +R+ ++ ++++ L V +M+ + G E+ NLA
Sbjct: 59 DWNSKVIFTMSD-VNVDSPNDKLREHAKEVFMRDVAWAEHLQNVGNLMVRLRGPENENLA 117
Query: 439 RIL 447
I+
Sbjct: 118 SIV 120
>UniRef50_Q9VZM5 Cluster: GPI mannosyltransferase 3; n=5;
Diptera|Rep: GPI mannosyltransferase 3 - Drosophila
melanogaster (Fruit fly)
Length = 561
Score = 34.7 bits (76), Expect = 2.1
Identities = 12/31 (38%), Positives = 21/31 (67%)
Frame = +2
Query: 557 FGNEPWYWWSQISMSALELGINVLGVVFGVV 649
+G+ PW+W+ + + + LGIN L +FGV+
Sbjct: 237 YGSHPWHWYFSVGLPTV-LGINTLPFIFGVM 266
>UniRef50_A6S0C6 Cluster: Putative uncharacterized protein; n=1;
Botryotinia fuckeliana B05.10|Rep: Putative
uncharacterized protein - Botryotinia fuckeliana B05.10
Length = 519
Score = 34.3 bits (75), Expect = 2.8
Identities = 17/50 (34%), Positives = 27/50 (54%)
Frame = +1
Query: 256 PQDWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMI 405
P D S+++A SP+I++ SP V LN E++Y GV ++I
Sbjct: 117 PSDTVSQLIAYSSPWIDLCSPDPLVANISRQVLNIEIAYASFCGVGNVII 166
>UniRef50_A3I251 Cluster: Putative uncharacterized protein; n=1;
Algoriphagus sp. PR1|Rep: Putative uncharacterized
protein - Algoriphagus sp. PR1
Length = 161
Score = 33.9 bits (74), Expect = 3.7
Identities = 18/49 (36%), Positives = 30/49 (61%), Gaps = 1/49 (2%)
Frame = +1
Query: 232 YAVRYGSIPQDWTSRIVAKLSPYI-NVDSPSATVRQRHEDYLNEELSYC 375
+A + G IP+ ++++ +L+ YI VD AT +E Y+NEE S+C
Sbjct: 85 FATQIGKIPE--STQLDERLTDYIIQVDGDMATAWTPYEFYVNEEFSHC 131
>UniRef50_A7EAP0 Cluster: Putative uncharacterized protein; n=2;
Sclerotiniaceae|Rep: Putative uncharacterized protein -
Sclerotinia sclerotiorum 1980
Length = 811
Score = 33.9 bits (74), Expect = 3.7
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +1
Query: 256 PQDWTSRIVAKLSPYINVDSPSATVRQRHEDYLNEELSYCRGLGVPAIMI 405
P D S+++A SP+I++ SP + LN E++Y GV ++I
Sbjct: 117 PSDTVSQLIAYSSPWIDLCSPDPLIANISRQVLNIEIAYASFCGVGNVII 166
>UniRef50_Q1F0S7 Cluster: Beta-xylosidase-like; n=1; Clostridium
oremlandii OhILAs|Rep: Beta-xylosidase-like -
Clostridium oremlandii OhILAs
Length = 854
Score = 33.5 bits (73), Expect = 4.9
Identities = 14/50 (28%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +1
Query: 268 TSRIVAKLSPYINVDSPSATVRQRHEDY-LNEELSYCRGLGVPAIMISIH 414
T+ + ++P + V P AT + Y L L+YC+ +P +S+H
Sbjct: 511 TALTIKSIAPTLKVGGPGATYHMNQQSYWLEIFLTYCKDYSIPLDFVSLH 560
>UniRef50_Q5CVH1 Cluster: SANT domain containing protein; n=2;
Cryptosporidium|Rep: SANT domain containing protein -
Cryptosporidium parvum Iowa II
Length = 1632
Score = 32.7 bits (71), Expect = 8.6
Identities = 19/43 (44%), Positives = 22/43 (51%)
Frame = +1
Query: 355 NEELSYCRGLGVPAIMISIHGRESNNLARILQTYYETSHHPSL 483
N E R LGV I I N L+ IL+ Y +TSHHP L
Sbjct: 648 NSEEEAIRDLGVSLIAFIIRLDVMNCLSWILKHYTKTSHHPEL 690
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 737,689,121
Number of Sequences: 1657284
Number of extensions: 15263442
Number of successful extensions: 37817
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 36648
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 37809
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 53719013270
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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