SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060255.seq
         (657 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.16c |dbp2||ATP-dependent RNA helicase Dbp2|Schizosacchar...   117   1e-27
SPCC10H11.01 |prp11||ATP-dependent RNA helicase Prp11|Schizosacc...    56   4e-09
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch...    45   8e-06
SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3 |Schizosaccha...    41   2e-04
SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su...    40   4e-04
SPAC823.08c |||ATP-dependent RNA helicase Rrp3 |Schizosaccharomy...    36   0.007
SPAC1F5.10 |||ATP-dependent RNA helicase, eIF4A related|Schizosa...    33   0.048
SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha...    32   0.063
SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomy...    29   0.59 
SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|...    29   0.59 
SPCC1494.06c |||ATP-dependent RNA helicase Dbp9 |Schizosaccharom...    27   2.4  
SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac...    26   5.5  
SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2 |...    25   7.3  
SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual       25   7.3  
SPCC1795.09 |yps1||aspartic protease Yps1|Schizosaccharomyces po...    25   9.6  
SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ...    25   9.6  

>SPBP8B7.16c |dbp2||ATP-dependent RNA helicase
           Dbp2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 550

 Score =  117 bits (282), Expect = 1e-27
 Identities = 58/126 (46%), Positives = 74/126 (58%)
 Frame = +3

Query: 255 WDSVSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQYFEEANFPDYV 434
           W + +L PF K+FY  H  V  RS  EV EYR   E+ V G+ V  P+  FEEA FP+YV
Sbjct: 74  WKNETLIPFQKDFYKEHENVRNRSDAEVTEYRKEKEIVVHGLNVPKPVTTFEEAGFPNYV 133

Query: 435 QXGVKTMGYKEPTPIQAQGWPIAMSERI*LAVAQXGVXAKRCPTSXQAIVHINNXPAIRR 614
              VK +G++ PTPIQ Q WP+AMS R  + ++  G   K       AIVHIN  P +  
Sbjct: 134 LKEVKQLGFEAPTPIQQQAWPMAMSGRDMVGISATG-SGKTLSYCLPAIVHINAQPLLSP 192

Query: 615 GDGPIL 632
           GDGPI+
Sbjct: 193 GDGPIV 198


>SPCC10H11.01 |prp11||ATP-dependent RNA helicase
           Prp11|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1014

 Score = 56.0 bits (129), Expect = 4e-09
 Identities = 33/123 (26%), Positives = 59/123 (47%), Gaps = 1/123 (0%)
 Frame = +3

Query: 264 VSLQPFNKNFYDPHPTVLKRSPYEVEEYRNNHE-VTVSGVEVHNPIQYFEEANFPDYVQX 440
           ++ + F K+FY     +   SP EV+E R + + + + G++   P+  + +         
Sbjct: 372 INYEDFKKDFYVEPEELKNLSPAEVDELRASLDGIKIRGIDCPKPVTSWSQCGLSAQTIS 431

Query: 441 GVKTMGYKEPTPIQAQGWPIAMSERI*LAVAQXGVXAKRCPTSXQAIVHINNXPAIRRGD 620
            + ++GY++PT IQAQ  P   S R  + VA+ G   K          HI +   ++ G+
Sbjct: 432 VINSLGYEKPTSIQAQAIPAITSGRDVIGVAKTG-SGKTIAFLLPMFRHIKDQRPLKTGE 490

Query: 621 GPI 629
           GPI
Sbjct: 491 GPI 493


>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 45.2 bits (102), Expect = 8e-06
 Identities = 21/87 (24%), Positives = 45/87 (51%)
 Frame = +3

Query: 348 RNNHEVTVSGVEVHNPIQYFEEANFPDYVQXGVKTMGYKEPTPIQAQGWPIAMSERI*LA 527
           + ++ +++ G ++ NP++ +EEA  P  +   +K + YKEP+ IQ    P+ +  +  + 
Sbjct: 232 KEDYNISIKGDDLPNPLRNWEEAGLPSEMLKVLKKVNYKEPSSIQRAAIPVLLQRKDLIG 291

Query: 528 VAQXGVXAKRCPTSXQAIVHINNXPAI 608
           +A+ G   K        I+ I+  P +
Sbjct: 292 IAETG-SGKTAAFIIPLIIAISKLPPL 317


>SPBC17D1.06 |dbp3||ATP-dependent RNA helicase Dbp3
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 578

 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 20/50 (40%), Positives = 30/50 (60%)
 Frame = +3

Query: 393 PIQYFEEANFPDYVQXGVKTMGYKEPTPIQAQGWPIAMSERI*LAVAQXG 542
           PI  F+E +    ++ G+K   YKEPTPIQA  WP  ++ R  + +A+ G
Sbjct: 165 PILQFDELDVSAKLREGLKN--YKEPTPIQAATWPYLLAGRDVVGIAETG 212


>SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase
           Sum3|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 636

 Score = 39.5 bits (88), Expect = 4e-04
 Identities = 21/60 (35%), Positives = 30/60 (50%)
 Frame = +3

Query: 363 VTVSGVEVHNPIQYFEEANFPDYVQXGVKTMGYKEPTPIQAQGWPIAMSERI*LAVAQXG 542
           V VSG ++  P+  F       ++   +K  GY +PTP+Q    PI  S R  +A AQ G
Sbjct: 158 VEVSGGDIE-PVNEFTSPPLNSHLLQNIKLSGYTQPTPVQKNSIPIVTSGRDLMACAQTG 216


>SPAC823.08c |||ATP-dependent RNA helicase Rrp3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 465

 Score = 35.5 bits (78), Expect = 0.007
 Identities = 19/67 (28%), Positives = 34/67 (50%)
 Frame = +3

Query: 405 FEEANFPDYVQXGVKTMGYKEPTPIQAQGWPIAMSERI*LAVAQXGVXAKRCPTSXQAIV 584
           F+E    D +    + +G+K PTPIQ +  P+ +++R  + +AQ G   K    +   I 
Sbjct: 48  FKELGVIDELCEACEKLGFKTPTPIQQEAIPVVLNKRDVIGLAQTG-SGKTAAFALPVIQ 106

Query: 585 HINNXPA 605
            + N P+
Sbjct: 107 ELWNNPS 113


>SPAC1F5.10 |||ATP-dependent RNA helicase, eIF4A
           related|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 394

 Score = 32.7 bits (71), Expect = 0.048
 Identities = 21/68 (30%), Positives = 33/68 (48%)
 Frame = +3

Query: 339 EEYRNNHEVTVSGVEVHNPIQYFEEANFPDYVQXGVKTMGYKEPTPIQAQGWPIAMSERI 518
           +E   N E+T S  E  N +  FEE N  + +  G+   GY+ P+ +Q++        R 
Sbjct: 3   DEIMENVELTTS--EDVNAVSSFEEMNLKEDLLRGIYAYGYETPSAVQSRAIIQICKGRD 60

Query: 519 *LAVAQXG 542
            +A AQ G
Sbjct: 61  VIAQAQSG 68


>SPBC776.09 |ste13||ATP-dependent RNA helicase
           Ste13|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 485

 Score = 32.3 bits (70), Expect = 0.063
 Identities = 17/46 (36%), Positives = 26/46 (56%)
 Frame = +3

Query: 405 FEEANFPDYVQXGVKTMGYKEPTPIQAQGWPIAMSERI*LAVAQXG 542
           FE+      +  G+   G++ P+PIQ +  PIA+S R  LA A+ G
Sbjct: 46  FEDYYLKRELLMGIFEAGFERPSPIQEESIPIALSGRDILARAKNG 91


>SPBC24C6.02 |||ATP-dependent RNA helicase Spb4 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 606

 Score = 29.1 bits (62), Expect = 0.59
 Identities = 10/37 (27%), Positives = 20/37 (54%)
 Frame = +3

Query: 405 FEEANFPDYVQXGVKTMGYKEPTPIQAQGWPIAMSER 515
           F+  N   +++  V   G+K+ TP+QA   P+ +  +
Sbjct: 3   FQSINIDKWLKNAVAAQGFKKMTPVQANAIPLFLKNK 39


>SPAC17D4.03c |||membrane transporter |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 732

 Score = 29.1 bits (62), Expect = 0.59
 Identities = 9/37 (24%), Positives = 23/37 (62%)
 Frame = +2

Query: 158 RLDLATVAVDLEDLEDLVGKKNSLEVRTCVAQMGFCF 268
           + D++ +   +  + +    +NS+++ +C+ Q+GFCF
Sbjct: 274 KFDISLIMALISAIINKTASQNSIKIISCLYQVGFCF 310


>SPCC1494.06c |||ATP-dependent RNA helicase Dbp9
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 595

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 12/56 (21%), Positives = 29/56 (51%)
 Frame = +3

Query: 375 GVEVHNPIQYFEEANFPDYVQXGVKTMGYKEPTPIQAQGWPIAMSERI*LAVAQXG 542
           G+   +  + F + N    +Q  +    +++PT +Q++  P+A+  +  +A A+ G
Sbjct: 6   GIREESSEKTFSDFNLDPRLQRAIHKCEFEKPTSVQSETIPLALEGKDLVAQARTG 61


>SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 848

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +3

Query: 456 GYKEPTPIQAQGWPIAMSERI*LAVAQXG 542
           G+K PTPIQ +  P+ +  R  + +A+ G
Sbjct: 88  GFKAPTPIQRKTIPLLLEGRDVVGMARTG 116


>SPAC17A5.07c |ulp2||SUMO deconjugating cysteine peptidase Ulp2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 652

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 18/52 (34%), Positives = 25/52 (48%), Gaps = 4/52 (7%)
 Frame = +3

Query: 258 DSVSLQPFNKN----FYDPHPTVLKRSPYEVEEYRNNHEVTVSGVEVHNPIQ 401
           D V L PF K     FYDP P  LK + +E+     +  + V    +H P+Q
Sbjct: 112 DVVEL-PFTKGGYGGFYDPRPGCLKFTTHEINVSYTDTSIPV----IHIPVQ 158


>SPAC3H8.09c |nab3||poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 738

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 2/46 (4%)
 Frame = -2

Query: 476 RRRFFVTHCLY--TXLHIIRKICFFKVLNRIMNLNATYSYLVIISV 345
           RR    T CLY  + L +I K C    +  I+ +NA  ++L  +SV
Sbjct: 524 RRLNIHTTCLYRDSNLQVIIKSCIINSVKSIILINAGLAHLGKVSV 569


>SPCC1795.09 |yps1||aspartic protease Yps1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 521

 Score = 25.0 bits (52), Expect = 9.6
 Identities = 12/48 (25%), Positives = 23/48 (47%)
 Frame = +3

Query: 348 RNNHEVTVSGVEVHNPIQYFEEANFPDYVQXGVKTMGYKEPTPIQAQG 491
           R +  +TV+G+ V + + Y      PD+ Q G+    Y   + +  +G
Sbjct: 153 RYDDNITVAGINVQDSLSYSHYQALPDF-QFGITLKEYVPSSMLPYKG 199


>SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar
           membrane transporter Hmt1|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 830

 Score = 25.0 bits (52), Expect = 9.6
 Identities = 10/28 (35%), Positives = 17/28 (60%)
 Frame = +1

Query: 277 LSTKTFMIHILQFSKDHHMKSKSTEIIT 360
           +STK    H+L  S D H+  ++ E++T
Sbjct: 340 ISTKALR-HVLNLSYDFHLNKRAGEVLT 366


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,244,990
Number of Sequences: 5004
Number of extensions: 41089
Number of successful extensions: 100
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -