BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060245.seq (693 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizo... 59 7e-10 SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 29 0.64 SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 27 2.6 SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 3.4 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 4.5 SPAC23C11.13c |hpt1||xanthine phosphoribosyltransferase |Schizos... 25 7.9 SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 25 7.9 >SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 58.8 bits (136), Expect = 7e-10 Identities = 41/134 (30%), Positives = 61/134 (45%), Gaps = 1/134 (0%) Frame = +3 Query: 111 IFIVGAKRTPFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSMSWAKSSLHHKLT 290 ++IV A RTP G+FGG F + AT+L +IA GAL+ + P+ VD + + Sbjct: 6 VYIVSAVRTPMGSFGGSFASLPATKLGSIAIKGALERVNIKPSDVDEVFMGNVVSANLGQ 65 Query: 291 ESTRHVTRRSKLVFLKRSRCSASTDYAALVSNRS*TALRTF*PAH-KISVAGGVENMSQA 467 R + L + C+ A + +T + +I VAGG E+MS A Sbjct: 66 NPARQCALGAGLP--RSIVCTTVNKVCASGMKATILGAQTIMTGNAEIVVAGGTESMSNA 123 Query: 468 PFAVRNVRFGTALG 509 P+ RFG G Sbjct: 124 PYYAPKNRFGAKYG 137 Score = 42.7 bits (96), Expect = 5e-05 Identities = 22/71 (30%), Positives = 40/71 (56%) Frame = +2 Query: 257 VGQVISSSQTDGIYTPRHAALKAGIPQEKPVLGINRLCGSGFQSIVNSAQDILTGAQDLS 436 +G V+S++ G R AL AG+P+ +N++C SG ++ + AQ I+TG ++ Sbjct: 55 MGNVVSANL--GQNPARQCALGAGLPRSIVCTTVNKVCASGMKATILGAQTIMTGNAEIV 112 Query: 437 SGGSREHVASA 469 G E +++A Sbjct: 113 VAGGTESMSNA 123 >SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 29.1 bits (62), Expect = 0.64 Identities = 14/39 (35%), Positives = 20/39 (51%) Frame = +3 Query: 219 EAGVSPAQVDSMSWAKSSLHHKLTESTRHVTRRSKLVFL 335 E+ P+Q + +SW + H KL +ST SK FL Sbjct: 23 ESATKPSQQELISWFEEHFHKKLAQSTVSSILSSKNEFL 61 >SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1019 Score = 27.1 bits (57), Expect = 2.6 Identities = 16/59 (27%), Positives = 28/59 (47%) Frame = +3 Query: 168 NTSATELQTIATVGALKEAGVSPAQVDSMSWAKSSLHHKLTESTRHVTRRSKLVFLKRS 344 NTS+T+ + ++ + P Q+DS+ K ++ E R +RR FL R+ Sbjct: 630 NTSSTQPSQVDSLVPTQLDSTIPTQIDSVQRNKDQDDEEILEERRE-SRRDSKTFLSRT 687 >SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces pombe|chr 1|||Manual Length = 949 Score = 26.6 bits (56), Expect = 3.4 Identities = 10/44 (22%), Positives = 26/44 (59%), Gaps = 1/44 (2%) Frame = -3 Query: 409 VLSAVHDRLE-TRAA*SVDAEHRLLLRNTSFERRVTWRVDSVSL 281 + V + ++ T+ + + ++ LLL N+ + +++W +D+V L Sbjct: 185 IFDVVSEHIDITQTSDAPNSNRNLLLNNSMLQEKISWYLDTVEL 228 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/39 (33%), Positives = 19/39 (48%) Frame = +3 Query: 138 PFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSM 254 P G G + + T+ Q I + L V PAQ+DS+ Sbjct: 270 PSGPMGMKTGSVNDTQSQQITLMNVLASQNVPPAQIDSI 308 >SPAC23C11.13c |hpt1||xanthine phosphoribosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 206 Score = 25.4 bits (53), Expect = 7.9 Identities = 13/48 (27%), Positives = 25/48 (52%) Frame = +3 Query: 180 TELQTIATVGALKEAGVSPAQVDSMSWAKSSLHHKLTESTRHVTRRSK 323 T+ +T+G + G + VD + +++LH+ L E R V ++K Sbjct: 88 TQWLDFSTLGMVDLVGKNILIVDEVDDTRTTLHYALRELQRDVAEQAK 135 >SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1063 Score = 25.4 bits (53), Expect = 7.9 Identities = 12/46 (26%), Positives = 23/46 (50%) Frame = -3 Query: 184 SVADVLRKTPPKVPNGVLLAPTMKIPLTEADMIQNYTYPVNVELKY 47 SV+ +R +PN P P +A+ + N ++PV+ +L + Sbjct: 755 SVSSNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKLAF 800 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,973,512 Number of Sequences: 5004 Number of extensions: 62807 Number of successful extensions: 149 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 142 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 321951680 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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