BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060241.seq (575 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 124 7e-30 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 124 7e-30 SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 3.4 SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltra... 26 4.5 SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 25 6.0 SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pomb... 25 6.0 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 124 bits (300), Expect = 7e-30 Identities = 59/91 (64%), Positives = 69/91 (75%) Frame = +1 Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFXPTKPXRRWHRRCQPX 435 GHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMF PTK R+WH + Sbjct: 59 GHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQN 118 Query: 436 DSGERPWPAAVAATGVPALVXAXRNIIEKIP 528 + +AVAA+GVP+L+ A + IE+IP Sbjct: 119 EK-RYAIASAVAASGVPSLLLARGHRIEEIP 148 Score = 69.7 bits (163), Expect = 3e-13 Identities = 32/58 (55%), Positives = 42/58 (72%) Frame = +2 Query: 80 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 253 ++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++ Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 124 bits (300), Expect = 7e-30 Identities = 59/91 (64%), Positives = 69/91 (75%) Frame = +1 Query: 256 GHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFXPTKPXRRWHRRCQPX 435 GHQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMF PTK R+WH + Sbjct: 59 GHQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQN 118 Query: 436 DSGERPWPAAVAATGVPALVXAXRNIIEKIP 528 + +AVAA+GVP+L+ A + IE+IP Sbjct: 119 EK-RYAISSAVAASGVPSLLLARGHRIEEIP 148 Score = 70.5 bits (165), Expect = 2e-13 Identities = 33/58 (56%), Positives = 42/58 (72%) Frame = +2 Query: 80 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKE 253 ++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++ Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEK 57 >SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr 2|||Manual Length = 606 Score = 26.2 bits (55), Expect = 3.4 Identities = 10/26 (38%), Positives = 17/26 (65%) Frame = -3 Query: 141 APCTVSLFSEYTDTKGRATDRLISLL 64 A C V ++SEYT G ++ L++L+ Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515 >SPAC1D4.08 |pis1||CDP-diacylglycerol--inositol 3-phosphatidyltransferase Pis1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 251 Score = 25.8 bits (54), Expect = 4.5 Identities = 14/28 (50%), Positives = 20/28 (71%), Gaps = 2/28 (7%) Frame = -1 Query: 98 KVG-LPIDSFRYFSEAIPLKY-TLIAVI 21 K+G LP+ SF Y + +PL Y TL+AV+ Sbjct: 186 KLGYLPVPSFIYSTGELPLSYPTLLAVL 213 >SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit Bgs4|Schizosaccharomyces pombe|chr 3|||Manual Length = 1955 Score = 25.4 bits (53), Expect = 6.0 Identities = 11/43 (25%), Positives = 20/43 (46%) Frame = +1 Query: 391 PTKPXRRWHRRCQPXDSGERPWPAAVAATGVPALVXAXRNIIE 519 PT + +H+R P +G W A + V ++ A ++ E Sbjct: 536 PTLYTKPFHQRDGPKPTGASQWAAVACTSVVSCIIMAAASLCE 578 >SPBC17D11.01 |nep1||nedd8 protease Nep1|Schizosaccharomyces pombe|chr 2|||Manual Length = 420 Score = 25.4 bits (53), Expect = 6.0 Identities = 12/45 (26%), Positives = 20/45 (44%) Frame = -2 Query: 427 DSDGANAXRASWGRTYVHHDTCYRRHPDRTYEYHHHGHAEFGRQH 293 +S +N ++S + HH+ + D +E HHH H H Sbjct: 267 ESSNSNLKKSSESGSTNHHNN-HESDKDLHHEGHHHHHHHHHHHH 310 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,109,781 Number of Sequences: 5004 Number of extensions: 39916 Number of successful extensions: 101 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 94 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 97 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 246098644 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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