BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060239.seq (656 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g19930.1 68414.m02498 kelch repeat-containing F-box family pr... 30 1.6 At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to ... 29 3.6 At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to ... 29 3.6 At3g12500.1 68416.m01556 basic endochitinase identical to basic ... 28 4.8 >At1g19930.1 68414.m02498 kelch repeat-containing F-box family protein contains F-box domain Pfam:PF00646 and Kelch motif Pfam:PF01344 Length = 344 Score = 29.9 bits (64), Expect = 1.6 Identities = 12/25 (48%), Positives = 18/25 (72%) Frame = +1 Query: 340 KRPRRSLDSPRLDGYVLASSPIPHT 414 +RP + D+ + GYVLA+ PIPH+ Sbjct: 84 RRPILASDTRKSSGYVLATIPIPHS 108 >At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 383 Score = 28.7 bits (61), Expect = 3.6 Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 8/50 (16%) Frame = +1 Query: 334 VDKRPRRSLDSPRLD-------GYVLASSPIPHTDWNEELKL-YAQSHGY 459 VD+ RS R+D GY ++ SP P W E +L + HGY Sbjct: 102 VDREADRSYKRARIDTIAGGSPGYGVSESPSPRISWENERRLKMVRDHGY 151 >At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to nuclear protein ZAP, Mus musculus, EMBL:AB033168 this cDNA provides a truncated ORF likely due to a skipped exon. An alternative ORF is provided. Length = 661 Score = 28.7 bits (61), Expect = 3.6 Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 8/50 (16%) Frame = +1 Query: 334 VDKRPRRSLDSPRLD-------GYVLASSPIPHTDWNEELKL-YAQSHGY 459 VD+ RS R+D GY ++ SP P W E +L + HGY Sbjct: 102 VDREADRSYKRARIDTIAGGSPGYGVSESPSPRISWENERRLKMVRDHGY 151 >At3g12500.1 68416.m01556 basic endochitinase identical to basic endochitinase precursor SP:P19171 from [Arabidopsis thaliana] Length = 322 Score = 28.3 bits (60), Expect = 4.8 Identities = 11/46 (23%), Positives = 19/46 (41%) Frame = -1 Query: 455 PWLWAYSFNSSFQSVCGMGDDAKTYPSKRGESKERRGRLSTTWLFN 318 P+ W Y F + + T+P G+ RG + +W +N Sbjct: 150 PYSWGYCFKQEQNPASDYCEPSATWPCASGKRYYGRGPMQLSWNYN 195 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,842,237 Number of Sequences: 28952 Number of extensions: 212591 Number of successful extensions: 603 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 587 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 603 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1373722560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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