BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060234.seq (538 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g51860.1 68414.m05846 leucine-rich repeat protein kinase, put... 33 0.12 At5g19000.1 68418.m02257 speckle-type POZ protein-related contai... 31 0.49 At1g01640.2 68414.m00082 speckle-type POZ protein-related contai... 30 0.85 At1g01640.1 68414.m00081 speckle-type POZ protein-related contai... 30 0.85 At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 29 2.0 At3g27480.1 68416.m03436 DC1 domain-containing protein contains ... 29 2.0 At2g30600.2 68415.m03729 BTB/POZ domain-containing protein conta... 29 2.0 At2g30600.1 68415.m03728 BTB/POZ domain-containing protein conta... 29 2.0 At5g60920.1 68418.m07642 phytochelatin synthetase, putative / CO... 29 2.6 At2g39760.1 68415.m04882 speckle-type POZ protein-related contai... 29 2.6 At1g30370.1 68414.m03713 lipase class 3 family protein similar t... 28 3.4 At5g15430.1 68418.m01806 calmodulin-binding protein-related has ... 28 4.5 At3g57130.1 68416.m06360 ankyrin repeat family protein / BTB/POZ... 28 4.5 At3g16650.1 68416.m02128 PP1/PP2A phosphatases pleiotropic regul... 28 4.5 At1g21660.1 68414.m02711 expressed protein low similarity to SP|... 28 4.5 At5g13060.1 68418.m01497 armadillo/beta-catenin repeat family pr... 27 6.0 At3g46800.1 68416.m05080 DC1 domain-containing protein contains ... 27 6.0 At3g29810.1 68416.m03794 phytochelatin synthetase family protein... 27 6.0 At2g41370.1 68415.m05106 ankyrin repeat family protein / BTB/POZ... 27 6.0 At1g23160.1 68414.m02894 auxin-responsive GH3 family protein sim... 27 6.0 At5g13320.1 68418.m01531 auxin-responsive GH3 family protein sim... 27 7.9 >At1g51860.1 68414.m05846 leucine-rich repeat protein kinase, putative similar to light repressible receptor protein kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376; contains leucine rich repeat (LRR) domains, Pfam:PF00560; contains protein kinase domain, Pfam:PF00069 Length = 890 Score = 33.1 bits (72), Expect = 0.12 Identities = 23/59 (38%), Positives = 31/59 (52%), Gaps = 3/59 (5%) Frame = +3 Query: 336 FAEMKSLLDFMYKGEVNVGQNMLPMFLK---TAESLQVRGLTENNTLNPKSEERSTPVV 503 FAEMKSL G N+ +P L+ ++SL + L EN TL PK E + P+V Sbjct: 455 FAEMKSLKLINLSGNPNLNLTAIPDSLQQRVNSKSLTLI-LGENLTLTPKKESKKVPMV 512 >At5g19000.1 68418.m02257 speckle-type POZ protein-related contains Pfam PF00651 : BTB/POZ domain; contains Pfam PF00917: MATH domain; similar to Speckle-type POZ protein (SP:O43791) [Homo sapiens] Length = 442 Score = 31.1 bits (67), Expect = 0.49 Identities = 14/34 (41%), Positives = 19/34 (55%) Frame = +1 Query: 151 NNLTDVLASLLQREALCDVTLACDGETVKAHQTI 252 +NL L +LL+ CDV DGET AH+ + Sbjct: 187 SNLGQQLGNLLESGKGCDVVFQVDGETFNAHKLV 220 >At1g01640.2 68414.m00082 speckle-type POZ protein-related contains Pfam profile:PF00651 BTB/POZ domain; similar to Speckle-type POZ protein (SP:O43791) [Homo sapiens] Length = 207 Score = 30.3 bits (65), Expect = 0.85 Identities = 15/51 (29%), Positives = 23/51 (45%) Frame = +3 Query: 303 PHPIIFLKDVRFAEMKSLLDFMYKGEVNVGQNMLPMFLKTAESLQVRGLTE 455 P I L D+ E+KSLL+F+Y G + N A+ + L + Sbjct: 64 PEESITLPDLSHDELKSLLEFLYSGNLKAPYNQYRSLYLAADKYDISYLQD 114 >At1g01640.1 68414.m00081 speckle-type POZ protein-related contains Pfam profile:PF00651 BTB/POZ domain; similar to Speckle-type POZ protein (SP:O43791) [Homo sapiens] Length = 207 Score = 30.3 bits (65), Expect = 0.85 Identities = 15/51 (29%), Positives = 23/51 (45%) Frame = +3 Query: 303 PHPIIFLKDVRFAEMKSLLDFMYKGEVNVGQNMLPMFLKTAESLQVRGLTE 455 P I L D+ E+KSLL+F+Y G + N A+ + L + Sbjct: 64 PEESITLPDLSHDELKSLLEFLYSGNLKAPYNQYRSLYLAADKYDISYLQD 114 >At5g15630.1 68418.m01829 phytochelatin synthetase family protein / COBRA cell expansion protein COBL4 similar to phytochelatin synthetase [Hordeum vulgare subsp. vulgare] GI:29570314; identified in Roudier, et al, Plant Phys. (2002) 130:538-548 (PMID:12376623); contains Pfam profile PF04833: Phytochelatin synthetase-like conserved region Length = 431 Score = 29.1 bits (62), Expect = 2.0 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = -3 Query: 152 LGWLFQRKQNCWSIVGANVSER 87 LGW + +K+ WS+VGA +E+ Sbjct: 66 LGWTWAKKEVIWSMVGAQTTEQ 87 >At3g27480.1 68416.m03436 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 604 Score = 29.1 bits (62), Expect = 2.0 Identities = 24/71 (33%), Positives = 32/71 (45%), Gaps = 1/71 (1%) Frame = +3 Query: 117 PAILLALEQPP*QSDRC-ACESSAKRGSL*CHSRL*WRDSQGTPDNISACSPYFESIFLQ 293 P L LEQ C AC G + H D+ GT D + CS + F+ Sbjct: 435 PLHLSVLEQSDDSPTACSACLQVCSEGFVYDH------DTYGTFDLL--CSS-IDVPFIH 485 Query: 294 NSHPHPIIFLK 326 SHPHP+++LK Sbjct: 486 GSHPHPLLYLK 496 >At2g30600.2 68415.m03729 BTB/POZ domain-containing protein contains Pfam PF00651: BTB/POZ domain; contains Interpro IPR000210/ PS50097: BTBB/POZ domain; similar to MigA (GI:1841872) [Dictyostelium discoideum] Length = 809 Score = 29.1 bits (62), Expect = 2.0 Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 2/48 (4%) Frame = +3 Query: 252 ISACSPYFESIFLQN-SHPHP-IIFLKDVRFAEMKSLLDFMYKGEVNV 389 +S S F +F S H I+L DV K++++FMY GE+N+ Sbjct: 371 LSLWSVAFAKMFTNGMSESHSSTIYLTDVSPEAFKAMMNFMYSGELNM 418 >At2g30600.1 68415.m03728 BTB/POZ domain-containing protein contains Pfam PF00651: BTB/POZ domain; contains Interpro IPR000210/ PS50097: BTBB/POZ domain; similar to MigA (GI:1841872) [Dictyostelium discoideum] Length = 809 Score = 29.1 bits (62), Expect = 2.0 Identities = 17/48 (35%), Positives = 26/48 (54%), Gaps = 2/48 (4%) Frame = +3 Query: 252 ISACSPYFESIFLQN-SHPHP-IIFLKDVRFAEMKSLLDFMYKGEVNV 389 +S S F +F S H I+L DV K++++FMY GE+N+ Sbjct: 371 LSLWSVAFAKMFTNGMSESHSSTIYLTDVSPEAFKAMMNFMYSGELNM 418 >At5g60920.1 68418.m07642 phytochelatin synthetase, putative / COBRA cell expansion protein COB, putative similar to phytochelatin synthetase GI:29570314; similar to GB:AAK56072; identified in Roudier, et al, Plant Phys. (2002) 130:538-548 (PMID:12376623); identical to cDNA putative phytochelatin synthetase GI:3559804; contains Pfam profile PF04833: Phytochelatin synthetase-like conserved region Length = 456 Score = 28.7 bits (61), Expect = 2.6 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = -3 Query: 152 LGWLFQRKQNCWSIVGANVSER 87 LGW + +K+ WS+VGA +E+ Sbjct: 82 LGWKWAKKEVIWSMVGAQTTEQ 103 >At2g39760.1 68415.m04882 speckle-type POZ protein-related contains Pfam PF00651 : BTB/POZ domain; contains Pfam PF00917: MATH domain; similar to Speckle-type POZ protein (SP:O43791) [Homo sapiens] Length = 408 Score = 28.7 bits (61), Expect = 2.6 Identities = 14/44 (31%), Positives = 20/44 (45%) Frame = +1 Query: 151 NNLTDVLASLLQREALCDVTLACDGETVKAHQTIFQHAHHILKA 282 +N+ L LL E CD+ ET KAH+ I + +A Sbjct: 178 SNMGQGLKDLLDSEVGCDIAFQVGDETYKAHKLILAARSPVFRA 221 >At1g30370.1 68414.m03713 lipase class 3 family protein similar to DEFECTIVE IN ANTHER DEHISCENCE1 [Arabidopsis thaliana] GI:16215706; contains Pfam profile: PF01764: Lipase Length = 529 Score = 28.3 bits (60), Expect = 3.4 Identities = 16/42 (38%), Positives = 28/42 (66%), Gaps = 2/42 (4%) Frame = -1 Query: 469 FN-VLFSVKPLTCKLSAVFRNIGS-IFWPTFTSPLYIKSSND 350 FN VL + P+T +L+ V+R++G+ + F+SP Y+K +D Sbjct: 396 FNKVLNKLNPITSRLNWVYRHVGTQLKLDVFSSP-YVKRDSD 436 >At5g15430.1 68418.m01806 calmodulin-binding protein-related has weak similarity to calmodulin-binding proteins Length = 478 Score = 27.9 bits (59), Expect = 4.5 Identities = 13/27 (48%), Positives = 18/27 (66%) Frame = -2 Query: 240 CLDCLSITSESDITESLSLQKTRKHIG 160 C CL ES+ + S+ L+KTRKH+G Sbjct: 227 CSRCLKTKKESN-SLSVPLKKTRKHVG 252 >At3g57130.1 68416.m06360 ankyrin repeat family protein / BTB/POZ domain-containing protein contains Pfam domain, PF00023: Ankyrin repeat and Pfam domain, PF00651: BTB/POZ domain Length = 467 Score = 27.9 bits (59), Expect = 4.5 Identities = 13/44 (29%), Positives = 21/44 (47%) Frame = +1 Query: 163 DVLASLLQREALCDVTLACDGETVKAHQTIFQHAHHILKAYSCK 294 D L L+ +A DVT + +G V AH+ I + + C+ Sbjct: 15 DYLNLLINGQAFSDVTFSVEGRLVHAHRCILAARSLFFRKFFCE 58 >At3g16650.1 68416.m02128 PP1/PP2A phosphatases pleiotropic regulator 2 (PRL2) identical to SP|Q39190 PP1/PP2A phosphatases pleiotropic regulator PRL2 {Arabidopsis thaliana}, GB:Q39190 from [Arabidopsis thaliana]; contains Pfam PF00400: WD domain, G-beta repeat (7 copies, 1 weak) Length = 479 Score = 27.9 bits (59), Expect = 4.5 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = -1 Query: 355 NDFISANRTSLRNIMGCGCEFCRNMLS 275 NDF+SA+ +++ EFC NMLS Sbjct: 350 NDFVSASADNIKKFSLPKGEFCHNMLS 376 >At1g21660.1 68414.m02711 expressed protein low similarity to SP|O14976 Cyclin G-associated kinase (EC 2.7.1.-) {Homo sapiens}; supporting cDNA gi|20466222|gb|AY099577.1| Length = 523 Score = 27.9 bits (59), Expect = 4.5 Identities = 13/40 (32%), Positives = 22/40 (55%) Frame = +2 Query: 410 VPKNCRKFTSXRFDREQYIESEVGRAIDASSGRENLSRAE 529 +P + R+DREQ +S V +A+ + R++ SR E Sbjct: 378 IPGESEERRKARWDREQRTKSRVAQAVADMNNRDHQSRIE 417 >At5g13060.1 68418.m01497 armadillo/beta-catenin repeat family protein / BTB/POZ domain-containing protein contains armadillo/beta-catenin-like repeats, Pfam:PF00514 and a BTB/POZ domain, Pfam:PF00651 Length = 709 Score = 27.5 bits (58), Expect = 6.0 Identities = 14/66 (21%), Positives = 32/66 (48%), Gaps = 2/66 (3%) Frame = +3 Query: 258 ACSPYFESIF--LQNSHPHPIIFLKDVRFAEMKSLLDFMYKGEVNVGQNMLPMFLKTAES 431 A S F ++F L + + ++R+ + ++ F+Y G +N+ +++ L A+ Sbjct: 560 ASSDIFRAMFDGLYKERNAQNVEIPNIRWEVFELMMKFIYSGRINIAKHLAKDLLVAADQ 619 Query: 432 LQVRGL 449 + GL Sbjct: 620 YLLEGL 625 >At3g46800.1 68416.m05080 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 682 Score = 27.5 bits (58), Expect = 6.0 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = +3 Query: 261 CSPYFESIFLQNSHPHPIIF 320 CS +E FL SHPHP+ F Sbjct: 499 CSSIYEP-FLHESHPHPLYF 517 >At3g29810.1 68416.m03794 phytochelatin synthetase family protein / COBRA cell expansion protein COBL2 similar to phytochelatin synthetase [Hordeum vulgare subsp. vulgare] GI:29570314; identified in Roudier, et al, Plant Phys. (2002) 130:538-548 (PMID:12376623); contains Pfam profile PF04833: Phytochelatin synthetase-like conserved region Length = 441 Score = 27.5 bits (58), Expect = 6.0 Identities = 9/22 (40%), Positives = 15/22 (68%) Frame = -3 Query: 152 LGWLFQRKQNCWSIVGANVSER 87 LGW + +K+ WS+VG +E+ Sbjct: 74 LGWSWMKKEVIWSMVGGQATEQ 95 >At2g41370.1 68415.m05106 ankyrin repeat family protein / BTB/POZ domain-containing protein contains Pfam domain, PF00023: Ankyrin repeat and Pfam domain, PF00651: BTB/POZ domain Length = 491 Score = 27.5 bits (58), Expect = 6.0 Identities = 13/43 (30%), Positives = 20/43 (46%) Frame = +1 Query: 163 DVLASLLQREALCDVTLACDGETVKAHQTIFQHAHHILKAYSC 291 D L L+ +A DVT + +G V AH+ I + + C Sbjct: 14 DFLNLLINGQAFSDVTFSVEGRLVHAHRCILAARSLFFRKFFC 56 >At1g23160.1 68414.m02894 auxin-responsive GH3 family protein similar to auxin-responsive GH3 product [Glycine max] GI:18591, auxin-responsive GH3 homologue [Arabidopsis thaliana] GI:11041726; contains Pfam profile PF03321: GH3 auxin-responsive promoter Length = 578 Score = 27.5 bits (58), Expect = 6.0 Identities = 15/46 (32%), Positives = 25/46 (54%) Frame = +3 Query: 321 LKDVRFAEMKSLLDFMYKGEVNVGQNMLPMFLKTAESLQVRGLTEN 458 +K VR SL+D+ ++GQ P +K+ ++L+V L EN Sbjct: 527 IKVVRQGTFDSLMDYFISQGASIGQYKTPRCIKSGKALEV--LEEN 570 >At5g13320.1 68418.m01531 auxin-responsive GH3 family protein similar to auxin-responsive GH3 product [Glycine max] GI:18591; contains Pfam profile PF03321: GH3 auxin-responsive promoter Length = 575 Score = 27.1 bits (57), Expect = 7.9 Identities = 13/40 (32%), Positives = 21/40 (52%) Frame = +3 Query: 321 LKDVRFAEMKSLLDFMYKGEVNVGQNMLPMFLKTAESLQV 440 ++ VR SL+DF + GQ P +K+ ++LQV Sbjct: 524 IRVVRQGTFDSLMDFFISQGASTGQYKTPRCIKSGKALQV 563 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,629,025 Number of Sequences: 28952 Number of extensions: 269531 Number of successful extensions: 876 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 849 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 872 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 993966856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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