Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060233.seq
         (669 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam...    29   0.61 
SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyc...    27   2.4  
SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase Pik3|Schizo...    27   3.2  
SPBC119.17 ||SPBC577.01|metallopeptidase|Schizosaccharomyces pom...    26   4.3  
SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    26   5.6  
SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces...    26   5.6  
SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces...    26   5.6  
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual    25   7.5  
SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr 1...    25   7.5  
SPBC1711.04 |||methylenetetrahydrofolate reductase |Schizosaccha...    25   9.9  
SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom...    25   9.9  
SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom...    25   9.9  

>SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 543

 Score = 29.1 bits (62), Expect = 0.61
 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 6/51 (11%)
 Frame = -2

Query: 437 FKFI-YNLFVPVSGFIFNDNQDH----VVF-SFNISFVHFYCVKFEHVLNL 303
           F FI + LF  +S   F   + H    +VF S +I F+H YC K    +NL
Sbjct: 375 FAFIGFTLFATLSSLFFKVLKIHRRPIIVFGSLSIIFIHIYCFKITSWVNL 425


>SPAC23D3.14c |aah2||alpha-amylase homolog Aah2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 581

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 16/55 (29%), Positives = 28/55 (50%)
 Frame = +3

Query: 366 DDMVLVIVEDEPRDGYEEIVNKFEKNIEEECDVENILETAAKHGHFIELKLNLRK 530
           D+ +L+   D   +    ++ K+ KN+ +E D++ I   A KH   IE  L + K
Sbjct: 195 DENLLLADVDTENEVVLSVLEKWIKNVVQEYDIDGIRFDAIKHAP-IEFWLRMSK 248


>SPAC458.05 |pik3|vps34|phosphatidylinositol 3-kinase
           Pik3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 801

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 14/53 (26%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
 Frame = +3

Query: 375 VLVIVEDEPRDG-YEEIVNKFEKNIEEECDVENILETAAKHGHFIELKLNLRK 530
           ++V +EDEP    +  ++  F+K + +  +   I ET +    F+E  L + K
Sbjct: 416 LVVEIEDEPVSKLFSSVMFLFQKELSKSVEGRLIRETLSAQAKFVEKLLRISK 468


>SPBC119.17 ||SPBC577.01|metallopeptidase|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 992

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 13/34 (38%), Positives = 15/34 (44%)
 Frame = +3

Query: 168 SKFRNGLLGFDTFASLEIATKFKQFYCSGEPQKH 269
           SK RNG+    T+  L   T F    C G P  H
Sbjct: 797 SKERNGINSIKTYHELPFQTYFAAKSCLGVPYTH 830


>SPAC17A2.10c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 230

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 20/81 (24%), Positives = 34/81 (41%)
 Frame = -2

Query: 503 EMTVFRCSF*NILNITLLFYIFFKFIYNLFVPVSGFIFNDNQDHVVFSFNISFVHFYCVK 324
           E+++FRC    +    LLF++ F F ++    +S           +  F   F    CV 
Sbjct: 109 ELSLFRCLL--LFFFFLLFFLSFSFSFSFLFFLSQIFIVYFSSFPILHFLFFFFLCVCVF 166

Query: 323 FEHVLNLAFGHSNFVLLLMLL 261
              + +L+   S  +L L LL
Sbjct: 167 LSFLFSLSHLLSLAILFLPLL 187


>SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 609

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 19/69 (27%), Positives = 27/69 (39%)
 Frame = -1

Query: 228 SLQFPKMQRYQSLTTHYEILIITLVADTVQKGYVNHK*TIGFRAQNILQKISIVSFKNEE 49
           SL  P     Q L  H E     L+    Q GY + + T+  R  +   K+      NE 
Sbjct: 523 SLTIPMATEPQILEYHQE-WPQPLIQSIQQHGYSDPRQTLPTRLDSCAAKLREAVISNEN 581

Query: 48  IN*FHNFAL 22
           +N  H F +
Sbjct: 582 LNPSHKFGI 590


>SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 466

 Score = 25.8 bits (54), Expect = 5.6
 Identities = 11/25 (44%), Positives = 15/25 (60%)
 Frame = +3

Query: 396 EPRDGYEEIVNKFEKNIEEECDVEN 470
           E  + Y+EIV   +KN+  E DV N
Sbjct: 101 EAAEAYKEIVYDLQKNLASEMDVIN 125


>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 978

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 14/27 (51%), Positives = 17/27 (62%), Gaps = 2/27 (7%)
 Frame = +1

Query: 94  LSPEA--DCSFVIYITFLNSIRYKSDN 168
           LSPE   +CSF +Y  F   IRY SD+
Sbjct: 316 LSPELKENCSFQLYNLFPYLIRYLSDD 342


>SPAC521.04c |||calcium permease |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 881

 Score = 25.4 bits (53), Expect = 7.5
 Identities = 10/35 (28%), Positives = 20/35 (57%)
 Frame = -1

Query: 279 FVINAFGVHQNSKIV*ISLQFPKMQRYQSLTTHYE 175
           +++  FG H   K+  +S++ P    YQ+  +HY+
Sbjct: 244 YLLYPFGQHVRHKVKRLSVRSPAHPIYQTQHSHYD 278


>SPBC1711.04 |||methylenetetrahydrofolate reductase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 320

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 19/76 (25%), Positives = 35/76 (46%), Gaps = 3/76 (3%)
 Frame = +1

Query: 40  LIYFLVFKRHN*DFLQYILSPEADCSFVIYITFLN---SIRYKSDNQNFVMGC*ALIPLH 210
           +IYF VF      +LQ ++SP+ D   + +   +N   +IR+  D +          PL 
Sbjct: 98  MIYFPVFNDGQDQYLQQVVSPDKDVEGLCHKYVMNMYHNIRH-LDPEKTKKSILPCTPLA 156

Query: 211 LWKLQRNLNNFTVLVN 258
           + K+   L  +  ++N
Sbjct: 157 IVKILEYLGVYNKIIN 172


>SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces
           pombe|chr 1||Partial|Manual
          Length = 1887

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
 Frame = +3

Query: 336 KMDEADIKTEDDMVLVIVEDEP---RDGYEEIVNKFEKNI 446
           + D+ D  T+++   ++ +DE     +  EEIVN  EKN+
Sbjct: 344 RQDDVDQATDNNTNTILEDDEKDNDEEEEEEIVNAREKNL 383


>SPBCPT2R1.08c |tlh2||RecQ type DNA helicase
           Tlh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1919

 Score = 25.0 bits (52), Expect = 9.9
 Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
 Frame = +3

Query: 336 KMDEADIKTEDDMVLVIVEDEP---RDGYEEIVNKFEKNI 446
           + D+ D  T+++   ++ +DE     +  EEIVN  EKN+
Sbjct: 344 RQDDVDQATDNNTNTILEDDEKDNDEEEEEEIVNAREKNL 383


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,446,056
Number of Sequences: 5004
Number of extensions: 48860
Number of successful extensions: 161
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)