Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060231.seq
(670 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizo... 63 3e-11
SPAC17C9.03 |tif471||translation initiation factor eIF4G |Schizo... 30 0.35
SPBC14F5.12c |cbh2||centromere binding protein Cbh2|Schizosaccha... 28 1.4
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 2.4
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos... 27 3.2
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 4.3
SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 25 7.5
SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 25 9.9
SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|... 25 9.9
>SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 395
Score = 63.3 bits (147), Expect = 3e-11
Identities = 33/81 (40%), Positives = 49/81 (60%)
Frame = +1
Query: 256 VGQVISSSQTDGIYTPRHAALKAGIPQEKPVLGINRLCGSGFQSIVNSAQDILTGAAKIS 435
+G V+S++ G R AL AG+P+ +N++C SG ++ + AQ I+TG A+I
Sbjct: 55 MGNVVSANL--GQNPARQCALGAGLPRSIVCTTVNKVCASGMKATILGAQTIMTGNAEIV 112
Query: 436 VAGGVENMSQAPFAVRNVRFG 498
VAGG E+MS AP+ RFG
Sbjct: 113 VAGGTESMSNAPYYAPKNRFG 133
Score = 50.4 bits (115), Expect = 2e-07
Identities = 22/48 (45%), Positives = 32/48 (66%)
Frame = +2
Query: 110 IFIVGAKRTPFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSM 253
++IV A RTP G+FGG F + AT+L +IA GAL+ + P+ VD +
Sbjct: 6 VYIVSAVRTPMGSFGGSFASLPATKLGSIAIKGALERVNIKPSDVDEV 53
Score = 30.3 bits (65), Expect = 0.26
Identities = 14/26 (53%), Positives = 16/26 (61%)
Frame = +3
Query: 543 GLSDSYCGLPMGMTAEKLGAQFGITR 620
GLSD+Y GLPMG AE + I R
Sbjct: 149 GLSDAYDGLPMGNAAELCAEEHSIDR 174
>SPAC17C9.03 |tif471||translation initiation factor eIF4G
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1403
Score = 29.9 bits (64), Expect = 0.35
Identities = 22/61 (36%), Positives = 26/61 (42%)
Frame = +3
Query: 423 SQDLSSGGSREHVASALRGQKREVRHGSGTNYAFEDTLWAGLSDSYCGLPMGMTAEKLGA 602
SQ SSG R H RG R G G+N F + W+ D Y L G+ K G
Sbjct: 1275 SQRPSSG--RMHGRDMNRGDSR--MGGRGSNPPFSSSDWSNNKDGYARLGQGIRGLKSGT 1330
Query: 603 Q 605
Q
Sbjct: 1331 Q 1331
>SPBC14F5.12c |cbh2||centromere binding protein
Cbh2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 514
Score = 27.9 bits (59), Expect = 1.4
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +2
Query: 218 EAGVSPAQVDSMAWAKSSLHHKLTESTRHVTRRSKLVFL 334
E+ P+Q + ++W + H KL +ST SK FL
Sbjct: 23 ESATKPSQQELISWFEEHFHKKLAQSTVSSILSSKNEFL 61
>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 949
Score = 27.1 bits (57), Expect = 2.4
Identities = 11/52 (21%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
Frame = -2
Query: 432 DLGCAGQNVLSAVHDRLE-TRAA*SVDAEHRLLLRNTSFERRVTWRVDSVSL 280
D + V + ++ T+ + + ++ LLL N+ + +++W +D+V L
Sbjct: 177 DFNLDDPQIFDVVSEHIDITQTSDAPNSNRNLLLNNSMLQEKISWYLDTVEL 228
>SPAC694.06c |mrc1||mediator of replication checkpoint 1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1019
Score = 26.6 bits (56), Expect = 3.2
Identities = 16/59 (27%), Positives = 28/59 (47%)
Frame = +2
Query: 167 NTSATELQTIATVGALKEAGVSPAQVDSMAWAKSSLHHKLTESTRHVTRRSKLVFLKRS 343
NTS+T+ + ++ + P Q+DS+ K ++ E R +RR FL R+
Sbjct: 630 NTSSTQPSQVDSLVPTQLDSTIPTQIDSVQRNKDQDDEEILEERRE-SRRDSKTFLSRT 687
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 4.3
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +2
Query: 137 PFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSM 253
P G G + + T+ Q I + L V PAQ+DS+
Sbjct: 270 PSGPMGMKTGSVNDTQSQQITLMNVLASQNVPPAQIDSI 308
>SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 7.5
Identities = 12/46 (26%), Positives = 23/46 (50%)
Frame = -2
Query: 183 SVADVLRKTPPKVPNGVLLAPTMKIPLTEADMIQNYTYPVNVELKY 46
SV+ +R +PN P P +A+ + N ++PV+ +L +
Sbjct: 755 SVSSNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKLAF 800
>SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 743
Score = 25.0 bits (52), Expect = 9.9
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = +1
Query: 223 RSVPGSGRQHGVGQVISSSQTDGIYTPRHAALKAG-IPQEKPVLGINRLCGSGFQSIVNS 399
RS+P S + G S++Q + P A G +PQ P+ L G + + NS
Sbjct: 313 RSIPSSPKTEGAP---SNAQ----FRPSLPATPNGSVPQSNPLYDTTGLNGGQYPVVQNS 365
Query: 400 AQDIL 414
AQ +L
Sbjct: 366 AQPLL 370
>SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|chr
3|||Manual
Length = 521
Score = 25.0 bits (52), Expect = 9.9
Identities = 6/13 (46%), Positives = 12/13 (92%)
Frame = +1
Query: 61 VNWIGIILNHVCF 99
+NWI I+L+++C+
Sbjct: 421 INWINILLSYICY 433
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,710,106
Number of Sequences: 5004
Number of extensions: 53977
Number of successful extensions: 139
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 132
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 305854096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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