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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060223.seq
         (631 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g20280.1 68417.m02962 transcription initiation factor IID (TF...    32   0.27 
At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro...    31   0.48 
At4g34400.1 68417.m04886 transcriptional factor B3 family protei...    30   1.1  
At1g19990.1 68414.m02504 expressed protein ; expression supporte...    30   1.1  
At4g21660.1 68417.m03138 proline-rich spliceosome-associated (PS...    30   1.5  
At1g77840.1 68414.m09070 eukaryotic translation initiation facto...    29   2.5  
At3g18610.1 68416.m02365 nucleolin, putative contains Pfam profi...    29   3.4  
At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-...    28   5.9  
At1g03320.1 68414.m00311 hypothetical protein                          27   7.8  

>At4g20280.1 68417.m02962 transcription initiation factor IID
           (TFIID) 28 kDa subunit (TAFII-28) family protein similar
           to SP|Q15544 Transcription initiation factor TFIID 28
           kDa subunit (TAFII-28) (TAFII28) (TFIID subunit
           p30-beta) {Homo sapiens}; contains Pfam profile PF04719:
           hTAFII28-like protein conserved region
          Length = 210

 Score = 32.3 bits (70), Expect = 0.27
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = +3

Query: 300 PKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPAKLA 440
           P   AK S    ++  K  D  D+EE++N  +       SS PAK+A
Sbjct: 57  PMKKAKTSVVVTEAKNKDKDEDDEEEEENMDVELTKYPTSSDPAKMA 103


>At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1
           protein GI:1279562 from [Medicago sativa]
          Length = 557

 Score = 31.5 bits (68), Expect = 0.48
 Identities = 15/50 (30%), Positives = 23/50 (46%)
 Frame = +3

Query: 282 SDEEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPA 431
           S++E   KPAAK +    K      D SD++ +D K         ++K A
Sbjct: 164 SEDEPAKKPAAKIAKPAAKDSSSSDDDSDEDSEDEKPATKKAAPAAAKAA 213


>At4g34400.1 68417.m04886 transcriptional factor B3 family protein
           contains Pfam profile PF02362: B3 DNA binding domain
          Length = 389

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 14/34 (41%), Positives = 19/34 (55%)
 Frame = +3

Query: 288 EEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNK 389
           E  V KP   KS   GK  ++  + SDDEED+ +
Sbjct: 188 EFKVAKPTIPKSQKKGKKKEQVVESSDDEEDEEE 221


>At1g19990.1 68414.m02504 expressed protein ; expression supported
           by MPSS
          Length = 251

 Score = 30.3 bits (65), Expect = 1.1
 Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 7/66 (10%)
 Frame = +3

Query: 258 ENIKKVPVSDEEDVPKPA----AKKSXXXGK---SXKKGADRSDDEEDDNKSIINNTXNV 416
           E++K + + +E +    +    AKK    G    S K   + +DD++DDNK I ++    
Sbjct: 5   EDVKAMKMKEEAEEDNKSLSSFAKKKPTNGNNAGSKKLKKEENDDDDDDNKPIKSSVSGS 64

Query: 417 SSKPAK 434
            +KP K
Sbjct: 65  RAKPVK 70


>At4g21660.1 68417.m03138 proline-rich spliceosome-associated (PSP)
           family protein similar to SP|Q13435 Splicing factor 3B
           subunit 2 (Spliceosome associated protein 145) (SAP 145)
           (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa
           subunit) {Homo sapiens}; contains Pfam profiles PF04046:
           PSP, PF04037: Domain of unknown function (DUF382)
          Length = 584

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 16/54 (29%), Positives = 26/54 (48%)
 Frame = +3

Query: 276 PVSDEEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPAKL 437
           P++ EED  K  +++     K     +D  DDE+D+     N    +S+K  KL
Sbjct: 110 PLASEEDGTKDESEEKEDVKKKVNSDSDSDDDEQDNQ----NKEKGISNKKKKL 159


>At1g77840.1 68414.m09070 eukaryotic translation initiation factor
           5, putative / eIF-5, putative similar to SP|P55876
           Eukaryotic translation initiation factor 5 (eIF-5) {Zea
           mays}; contains Pfam profiles PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
           IF2B/IF5
          Length = 437

 Score = 29.1 bits (62), Expect = 2.5
 Identities = 14/40 (35%), Positives = 21/40 (52%)
 Frame = +3

Query: 258 ENIKKVPVSDEEDVPKPAAKKSXXXGKSXKKGADRSDDEE 377
           E +K+  ++DEE     A KK+   GK  K   + S DE+
Sbjct: 165 ERLKEGELADEEQRKLKAKKKALSNGKDSKTSKNHSSDED 204


>At3g18610.1 68416.m02365 nucleolin, putative contains Pfam profile:
           PF00076 RNA recognition motif
          Length = 636

 Score = 28.7 bits (61), Expect = 3.4
 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 3/54 (5%)
 Frame = +3

Query: 282 SDEEDVP--KPAA-KKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPAK 434
           SD+E  P  KP   K +    K      + SD+EE D++        VSSK +K
Sbjct: 274 SDDEPTPAKKPTVVKNAKPAAKDSSSSEEDSDEEESDDEKPPTKKAKVSSKTSK 327


>At1g72440.1 68414.m08377 CCAAT-box-binding transcription
           factor-related similar to CCAAT-box-binding
           transcription factor (CCAAT-binding factor) (CBF)
           (Swiss-Prot:Q03701) [Homo sapiens], GB:P53569 [Mus
           musculus]
          Length = 1056

 Score = 27.9 bits (59), Expect = 5.9
 Identities = 14/40 (35%), Positives = 20/40 (50%)
 Frame = +3

Query: 285 DEEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINN 404
           ++ D  KPA  K+    K  K    + +DEE + KS I N
Sbjct: 40  NDTDFRKPAKSKTQKRKKPKKDQQHKDEDEEGEPKSNIGN 79


>At1g03320.1 68414.m00311 hypothetical protein
          Length = 220

 Score = 27.5 bits (58), Expect = 7.8
 Identities = 14/50 (28%), Positives = 23/50 (46%)
 Frame = +3

Query: 285 DEEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPAK 434
           DEEDV +          K    G   SD+++  NK+++    + S+K  K
Sbjct: 27  DEEDVKQKQKVSKNENPKKDSNGEKGSDEKDKKNKNVLRWPQDSSTKKEK 76


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,241,750
Number of Sequences: 28952
Number of extensions: 139955
Number of successful extensions: 655
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 549
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 643
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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