BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060223.seq (631 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g20280.1 68417.m02962 transcription initiation factor IID (TF... 32 0.27 At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 pro... 31 0.48 At4g34400.1 68417.m04886 transcriptional factor B3 family protei... 30 1.1 At1g19990.1 68414.m02504 expressed protein ; expression supporte... 30 1.1 At4g21660.1 68417.m03138 proline-rich spliceosome-associated (PS... 30 1.5 At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 29 2.5 At3g18610.1 68416.m02365 nucleolin, putative contains Pfam profi... 29 3.4 At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-... 28 5.9 At1g03320.1 68414.m00311 hypothetical protein 27 7.8 >At4g20280.1 68417.m02962 transcription initiation factor IID (TFIID) 28 kDa subunit (TAFII-28) family protein similar to SP|Q15544 Transcription initiation factor TFIID 28 kDa subunit (TAFII-28) (TAFII28) (TFIID subunit p30-beta) {Homo sapiens}; contains Pfam profile PF04719: hTAFII28-like protein conserved region Length = 210 Score = 32.3 bits (70), Expect = 0.27 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = +3 Query: 300 PKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPAKLA 440 P AK S ++ K D D+EE++N + SS PAK+A Sbjct: 57 PMKKAKTSVVVTEAKNKDKDEDDEEEEENMDVELTKYPTSSDPAKMA 103 >At1g48920.1 68414.m05480 nucleolin, putative similar to nuM1 protein GI:1279562 from [Medicago sativa] Length = 557 Score = 31.5 bits (68), Expect = 0.48 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = +3 Query: 282 SDEEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPA 431 S++E KPAAK + K D SD++ +D K ++K A Sbjct: 164 SEDEPAKKPAAKIAKPAAKDSSSSDDDSDEDSEDEKPATKKAAPAAAKAA 213 >At4g34400.1 68417.m04886 transcriptional factor B3 family protein contains Pfam profile PF02362: B3 DNA binding domain Length = 389 Score = 30.3 bits (65), Expect = 1.1 Identities = 14/34 (41%), Positives = 19/34 (55%) Frame = +3 Query: 288 EEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNK 389 E V KP KS GK ++ + SDDEED+ + Sbjct: 188 EFKVAKPTIPKSQKKGKKKEQVVESSDDEEDEEE 221 >At1g19990.1 68414.m02504 expressed protein ; expression supported by MPSS Length = 251 Score = 30.3 bits (65), Expect = 1.1 Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 7/66 (10%) Frame = +3 Query: 258 ENIKKVPVSDEEDVPKPA----AKKSXXXGK---SXKKGADRSDDEEDDNKSIINNTXNV 416 E++K + + +E + + AKK G S K + +DD++DDNK I ++ Sbjct: 5 EDVKAMKMKEEAEEDNKSLSSFAKKKPTNGNNAGSKKLKKEENDDDDDDNKPIKSSVSGS 64 Query: 417 SSKPAK 434 +KP K Sbjct: 65 RAKPVK 70 >At4g21660.1 68417.m03138 proline-rich spliceosome-associated (PSP) family protein similar to SP|Q13435 Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (SAP 145) (SF3b150) (Pre-mRNA splicing factor SF3b 145 kDa subunit) {Homo sapiens}; contains Pfam profiles PF04046: PSP, PF04037: Domain of unknown function (DUF382) Length = 584 Score = 29.9 bits (64), Expect = 1.5 Identities = 16/54 (29%), Positives = 26/54 (48%) Frame = +3 Query: 276 PVSDEEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPAKL 437 P++ EED K +++ K +D DDE+D+ N +S+K KL Sbjct: 110 PLASEEDGTKDESEEKEDVKKKVNSDSDSDDDEQDNQ----NKEKGISNKKKKL 159 >At1g77840.1 68414.m09070 eukaryotic translation initiation factor 5, putative / eIF-5, putative similar to SP|P55876 Eukaryotic translation initiation factor 5 (eIF-5) {Zea mays}; contains Pfam profiles PF02020: eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in IF2B/IF5 Length = 437 Score = 29.1 bits (62), Expect = 2.5 Identities = 14/40 (35%), Positives = 21/40 (52%) Frame = +3 Query: 258 ENIKKVPVSDEEDVPKPAAKKSXXXGKSXKKGADRSDDEE 377 E +K+ ++DEE A KK+ GK K + S DE+ Sbjct: 165 ERLKEGELADEEQRKLKAKKKALSNGKDSKTSKNHSSDED 204 >At3g18610.1 68416.m02365 nucleolin, putative contains Pfam profile: PF00076 RNA recognition motif Length = 636 Score = 28.7 bits (61), Expect = 3.4 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 3/54 (5%) Frame = +3 Query: 282 SDEEDVP--KPAA-KKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPAK 434 SD+E P KP K + K + SD+EE D++ VSSK +K Sbjct: 274 SDDEPTPAKKPTVVKNAKPAAKDSSSSEEDSDEEESDDEKPPTKKAKVSSKTSK 327 >At1g72440.1 68414.m08377 CCAAT-box-binding transcription factor-related similar to CCAAT-box-binding transcription factor (CCAAT-binding factor) (CBF) (Swiss-Prot:Q03701) [Homo sapiens], GB:P53569 [Mus musculus] Length = 1056 Score = 27.9 bits (59), Expect = 5.9 Identities = 14/40 (35%), Positives = 20/40 (50%) Frame = +3 Query: 285 DEEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINN 404 ++ D KPA K+ K K + +DEE + KS I N Sbjct: 40 NDTDFRKPAKSKTQKRKKPKKDQQHKDEDEEGEPKSNIGN 79 >At1g03320.1 68414.m00311 hypothetical protein Length = 220 Score = 27.5 bits (58), Expect = 7.8 Identities = 14/50 (28%), Positives = 23/50 (46%) Frame = +3 Query: 285 DEEDVPKPAAKKSXXXGKSXKKGADRSDDEEDDNKSIINNTXNVSSKPAK 434 DEEDV + K G SD+++ NK+++ + S+K K Sbjct: 27 DEEDVKQKQKVSKNENPKKDSNGEKGSDEKDKKNKNVLRWPQDSSTKKEK 76 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,241,750 Number of Sequences: 28952 Number of extensions: 139955 Number of successful extensions: 655 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 549 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 643 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1285411824 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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